Ultrastructural Studies on the Fusion Product between Protoplasts of Mung Bean and Adzuki Bean.

Iriawati,; Miyake, Hiroshi; Taniguchi, Takeshi; Takeoka, Yoji

doi:10.1626/jcs.65.330

Cited by 5 publications

(1 citation statement)

References 24 publications

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“…Al-Yasiri and Coyne (1966) found that crosses between V. angularis and V. mungo resulted in pod set, but the pods collapsed at an early stage of development. Iriawati et al (1996) electrofused mesophyll protoplasts of V. radiata with V. angularis and discovered that the fusion product was distinguishable from the parental protoplasts and divided after 5 days in culture.…”

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confidence: 99%

Partly fertile interspecific hybrid between a black gram × green gram derivative and an adzuki bean

2002

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A true‐breeding black gram (Vigna mungo) × green gram (Vigna radiata) derivative was reciprocally crossed with adzuki bean, Vigna angularis. Pollinated pistils were treated with gibberellic acid (GA3) (70 p.p.m.) 24 and 48 h after pollination. One fertile pod containing two hybrid seeds was obtained, when V. angularis was used as the male parent. One of these seeds germinated and a partly fertile interspecific hybrid was obtained. Its hybridity was confirmed at the seedling stage based on the presence of the green epicotyl colour of the adzuki bean. Pubescence of epicotyl, stem, leaf margins and pod, and the colour of the mature pod, characteristics of the black gram × green gram derivative, were also expressed in the hybrid. In addition, the hybrid exhibited two novel traits — the presence of racemose inflorescence and plant regeneration ability — not present in the parents. The study of this hybrid and four offspring revealed that green epicotyl colour of adzuki bean, and the pubescence of stem and leaf margins of the black gram × green gram derivatives were dominantly inherited traits. Colours of epicotyl and stem bases were found to be linked.

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confidence: 99%

Partly fertile interspecific hybrid between a black gram × green gram derivative and an adzuki bean

2002

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Protoplasts of Grain and Forage Legumes: Their Exploitation in Genetic Manipulation, Physiological Investigations and Plant-Pathogen Interactions

Davey

Marchant

Power

2003

Focus on Biotechnology

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Aspects of fusion combining ability of dihaploidSolanum tuberosum L.: influence of the cytoplasm

Frei

Stattmann²,

Lössl

et al. 1998

Potato Res

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SummaryCreation from 4x hybrid clones from protoplast fusion of 2x clones of potato was evaluated. Besides combined nuclear genomes, composition of the cytoplasm significantly influenced the phenotypic traits of hybrid clones. To ascertain the influence of parental cytoplasm on the success of protoplast fusion and regeneration of hybrid plants, data from 74 fusion combinations of 50 dihaploid clones were analyzed. The majority of dihaploid breeding clones belonged to the cytoplasm types Wet, TI3 and W~/.When the closely related mt types et, 13 and "/were used, fusion combinations had a better combining ability compared with more distantly related cytoplasms 8 and e. Fusions containing the same mitochondrial type (homofusions) were not superior to closely related mitochondrial types. However, homofusions of cytoplasm type Wet yielded significantly more hybrids than homofusions of type TI]. In general, parental cytoplasm types had little impact on the fusion combining behaviour. Thus the cytoplasm type of the fusion parents is not a suitable marker for predicting the combining ability in protoplast fusion experiments.

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Ultrastructural Studies on the Fusion Product between Protoplasts of Mung Bean and Adzuki Bean.

Cited by 5 publications

References 24 publications

Partly fertile interspecific hybrid between a black gram × green gram derivative and an adzuki bean

Partly fertile interspecific hybrid between a black gram × green gram derivative and an adzuki bean

Protoplasts of Grain and Forage Legumes: Their Exploitation in Genetic Manipulation, Physiological Investigations and Plant-Pathogen Interactions

Aspects of fusion combining ability of dihaploidSolanum tuberosum L.: influence of the cytoplasm

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