Ultrastructural study of Kupffer cells in teleost liver under normal and experimental conditions

Ferri, S; Sesso, Antônio

doi:10.1007/bf00210516

Cited by 14 publications

(8 citation statements)

References 14 publications

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“…Apparently absent in certain salmonids (McCuskey et al, 1986), resident sinusoidal macrophages appear to be present in ictalurid (Hinton and Pool, 1976; the present study), and perhaps other teleost species (Fujita et al, 1980;Ferri and Sesso, 1981;Hinton et al, 1984). It appears from the results of the present study that resident sinusoidal macrophages of the bullhead catfish liver share many of the ultrastructural and function characteristics of mammalian Kupffer cells.…”

Section: Discussionmentioning

confidence: 56%

“…In the present study, sinusoidal macrophages were only observed to be anchored to endothelial cells by numerous desmosomes or desmosome-like densities. Similar junctions between macrophages and sinusoidal endothelial cells have been reported in Zctalurus punctatus (Hinton and Pool, 1976) and Pimelodus maculatus (Ferri and Sesso, 1981). In other teleost species, junctions between macrophages and supporting cells seen in the atrium of the heart, spleen, and kidney have not been described.…”

Section: Discussionmentioning

confidence: 66%

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Resident sinusoidal macrophages in the liver of the brown bullhead (Ictalurus nebulosus): An ultrastructural, functional and cytochemical study

1987

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Ultrastructural, functional, and cytochemical characteristics of resident sinusoidal macrophages (RSM) in brown bullhead (Ictalurus nebulosus) liver were examined. Following perfusion fixation of the hepatic vascular bed, light micrographs revealed RSM that possessed multiple elongate cytoplasmic processes and frequently contained erythrocytes in various stages of degradation. Following brief perfusion fixation, light microscope examination of vibratome sections of bullhead liver reacted for peroxidase revealed intensely positive RSM. By transmission electron microscopy, peroxidase activity was localized to the nuclear envelope and cytoplasmic granules of RSM and in endothelial and perisinusoidal fat-storing cells. In cryostat sections of fresh-frozen liver, glucose-6-phosphate dehydrogenase (G-6-PDH) was uniformly distributed over hepatocytes, whereas intensely positive punctate staining for G-6-PDH was localized over RSM. To test for phagocytosis by RSM, latex beads (0.81 micron) were injected into a tributary of the hepatic portal vein 2 min prior to perfusion fixation. Latex beads appeared either singly or in dense aggregates within RSM. Ultrastructurally, RSM were characterized by an irregularly shaped, eccentrically located nucleus, electron-dense vacuoles, small patches of granular endoplasmic reticulum, a well-developed Golgi apparatus, elongated mitochondria, desmosomes or desmosome-like densities that served as a source of attachment to endothelial cells, and a centriole with radiating microtubules. Invaginations of the plasma membrane (vermiform processes) characteristic of mammalian Kupffer cells were not observed in bullhead RSM. The results indicated a resident cell population of sinusoidal macrophages in the bullhead liver with properties that partially resembled mammalian Kupffer cells. These results are important for the identification of the normal resident cells in the bullhead liver.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 66%

Resident sinusoidal macrophages in the liver of the brown bullhead (Ictalurus nebulosus): An ultrastructural, functional and cytochemical study

1987

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“…The endothelial cells in the liver of the teleost fish Pimelodus maculatus Lacepède are able to take up India ink particles (Ferri & Sesso, 1981). The present result revealed that the liver endothelial cells in platies cannot accumulate foreign ferritin.…”

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confidence: 46%

The uptake of foreign ferritin by macrophages in the spleen, trunk kidney and liver of platy

Leknes¹

2001

Journal of Fish Biology

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“…The Kupffer and the sinusoidal endothelial cells are linked by the spot desmosomes to provide a strong attachment and prevent the Kupffer cells from being washed away by the blood. Ferri and Sesso (1981) also reported Kupffer cells in the liver of Pimelodus maculatus, but Tanuma et al (1982) and Rocha et al (1994) stated that no classical Kupffer cells are present in the liver of Kareius bicoloratus and Salmo trutta fario, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…They also added that the livers of Pagothenia borchgrevinki, Trematomus bernacchii and Gymnodraco acuticeps, have a few number of the perisinusoidal cells that contain very few lipid droplets. Ferri and Sesso (1981) reported that the liver of Pimelodus maculates has polymorphic Kupffer cells, which contain vacuoles varying widely in diameter, density and shape. They also suggested that these Kupffer cells and the endothelial cells may represent two different functional states of the same cell type.…”

Section: Introductionmentioning

confidence: 99%

Comparative histological and ultrastructural studies on the liver and pancreas of Schilbe mystus and Labeo niloticus

Naguib

Ghafar

2009

Egypt. J. of Aquatic Biolo. and Fish.

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ABSTRACThe present investigation aims to illustrate the histological and ultrastructural differences of the liver and pancreas of a carnivorous fish, Schilbe mystus, and a herbivorous fish, Labeo niloticus. The histological examination of the liver of both studied species revealed that the liver is mainly composed of a continuous compact field of the hepatocytes. The hepatocyte-sinusoidal structure of the liver of Schilbe mystus consists of the solid form and tubular one, while that of Labeo niloticus consists of the tubular form and cord-like one.The ultrastructural examination of the liver of both studied species revealed that it is composed mainly of hepatocytes, Kupffer (macrophages) and to (fat-storing) cells. The hepatocytes of Schilbe mystus are characterized by the presence of numerous glycogen rosettes, numerous lipid droplets and a rough endoplasmic reticulum, while those of Labeo niloticus are characterized by the presence of numerous glycogen rosettes, a few lipid droplets and a rough endoplasmic reticulum. The hepatocytes of both studied species possess numerous microvilli, which extend into the space of Disse or into the bile canaliculi lumina.In both studied species, there is a prominent perisinusoidal space between the hepatocytes and blood sinusoids, the space of Disse. This space usually contains the Ito cells, which are stellate in shape and are characterized by the presence of lipid droplets.The bile canaliculi of Schilbe mystus are of the intercellular type, while those of Labeo niloticus are of both the intercellular and intracellular types.The histological investigation of the pancreas of both studied species revealed that there are three types: the compact, disseminated and intrahepatic types. This pancreas showed that it consists of a highly lobulated gland of the compound acinar type. The acinar (pancreatic) cells are pyramidal in shape; they possess a basophilic cytoplasm and eosinophilic zymogenous secretory granules.The ultrastructural investigation of the acinar cells of both studied species revealed two cell types: the dark and light acinar cells. These cells are characterized by the presence of numerous zymogenous secretory granules and a well-developed rough endoplasmic reticulum.

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Ultrastructural study of Kupffer cells in teleost liver under normal and experimental conditions

Cited by 14 publications

References 14 publications

Resident sinusoidal macrophages in the liver of the brown bullhead (Ictalurus nebulosus): An ultrastructural, functional and cytochemical study

Resident sinusoidal macrophages in the liver of the brown bullhead (Ictalurus nebulosus): An ultrastructural, functional and cytochemical study

The uptake of foreign ferritin by macrophages in the spleen, trunk kidney and liver of platy

Comparative histological and ultrastructural studies on the liver and pancreas of Schilbe mystus and Labeo niloticus

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