Ultrastructural study of lamellar and nucleolus‐like bodies in the harderian gland during postnatal development of the hamster (<i>Mesocricetus auratus</i>)

López, José M.; Tolivia, Jorge; Diaz, C.; Alvarez-Uría, M

doi:10.1002/ar.1092280303

Cited by 14 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…21,25). Like the LBs, the NLBs have been described in a previous work (Lopez et al, 1990). The cells contained abundant lipid vacuoles located chiefly in the apical half of the cytoplasm.…”

Section: Stage 1: Days 1-5mentioning

confidence: 61%

See 1 more Smart Citation

Postnatal development of the harderian gland in the Syrian golden hamster (Mesocricetus auratus): A light and electron microscopic study

1992

View full text Add to dashboard Cite

The main objective of the present investigation was to study the morphological and chronological aspects of the development of the Harderian gland in the Syrian golden hamster. Tissues were obtained from male and female hamsters at days 1,3,5,7,10,12,15,17,20,27,37,46, and 90 after birth and processed for light and transmission electron microscopy. The present observations indicate that a well-defined temporal sequence in microscopic and ultrastructural modification is recognizable in the development of the hamster Harderian gland. Four stages of development were proposed. Between days 1-5 (first stage), the gland shows characteristics of an immature structure. The glandular cells contain many free ribosomes, few and small organelles, and large irregular-shape nuclei. Between days 7-17 (second stage), there is a marked increase of organelles involved in synthesis and secretion. The gland begins the secretion of lipids and porphyrins, but no morphological differences between male and female glands are observed. Between days 20-36 (third stage), the morphological differences between the two sexes appear and progressively develop. In 45-day-old hamsters, the Harderian gland possesses the structural characteristics of adult glands, and further developmental changes are essentially quantitative in nature (fourth stage). At all stages of development, the population of secretory cells has a uniform appearance. The morphological results are discussed as well as the possible relationship of this temporal sequence with hormonal changes.

show abstract

“…21,25). Like the LBs, the NLBs have been described in a previous work (Lopez et al, 1990). The cells contained abundant lipid vacuoles located chiefly in the apical half of the cytoplasm.…”

Section: Stage 1: Days 1-5mentioning

confidence: 61%

“…21, 24). These structures, identified as lamellar bodies (LBs), have been described in an earlier work (Lopez et al, 1990). Briefly, they were formed by two different types of cisterns: One was often continuous with a surrounding RER cistern and presented a n intraluminal electronlucent material.…”

Section: Stage 1: Days 1-5mentioning

confidence: 99%

Postnatal development of the harderian gland in the Syrian golden hamster (Mesocricetus auratus): A light and electron microscopic study

1992

View full text Add to dashboard Cite

show abstract

“…In addition, the Harderian glands from female hamsters contain an average of 40 times more mast cells within the connective tissue than the male gland (Payne et al, 1982). Also, there are distinct ultrastructural differences between the sexes (Bucana and Nadakavukaren, 1972;Lopez et al, 1990).It is clear that androgens alone can induce and maintain the male-type gland in both male and female ham-sters (Hoffman, 1971;Payne et al, 1977;Sun and Nadakavukaren, 1980). The fact that ovariectomy has little effect in changing the female-associated characteristics (Spike et al, 1986) described above suggests that ovarian steroids might not be important in the genesis and maintenance of the female-type characteristics.…”

mentioning

confidence: 85%

mentioning

confidence: 99%

Female syrian hamster harderian gland: Development and effects of high environmental temperature and melatonin injections on histology and porphyrin deposits

et al. 1992

View full text Add to dashboard Cite

We have investigated the development of the Harderian glands of female Syrian hamsters from birth to 8 months of age. The effects of melatonin injections on Harderian gland histology and porphyrin deposits of female hamsters exposed to two different temperatures were also studied. The morphology of the Harderian glands from 30-day-old female hamsters resembled those of sexually mature adult animals. The intraluminal area occupied by porphyrins increased significantly between 20 to 30 days of age. However, the relative number of mast cells per mm2 rose between 30 to 90 days. Both porphyrins and mast cell numbers appeared clearly reduced in the 8-month-old group. Secretory cells characterized by large lipid droplets (type II cells) were not observed. Daily afternoon injections of 25 micrograms of melatonin to female hamsters exposed to 22 degrees C for 14 weeks resulted in the discontinuity of estrous cyclicity, a marked decrease in the intraluminal area occupied by porphyrins, a reduction of the number of mast cells per mm2, and in a marked augmentation of the number of type II cells per mm2. Although the administration of similar dosages of melatonin to hamsters exposed to an environmental temperature of 32 degrees C did not interrupt estrous cycles, a clear reduction of the area occupied by porphyrins was observed. However, the number of mast cells and type II cells per mm2 was unaffected in these animals. Our results suggest that hormones other than ovarian steroids are involved in the regulation of the female hamster Harderian glands. The possible role of melatonin on Harderian gland metabolism is discussed.

show abstract

“…In most tissues, protoporphyrin IX is converted to heme by ferrochelatase. However, in the harderian glands the activity of this enzyme is very low [4] and thus, porphyrin concentrations may be elevated [5, 6] leading to an increase in oxidative stress [7]. Also, in the hamster harderian glands, mitochondrial damage and cell death have been demonstrated as a consequence of porphyrin accumulation [8, 9].…”

Section: Introductionmentioning

confidence: 99%

Characterization of melatonin high‐affinity binding sites in purified cell nuclei of the hamster (Mesocricetus auratus) harderian gland

Coto‐Montes

Tomás-Zapico

Escames

et al. 2003

Journal of Pineal Research

View full text Add to dashboard Cite

: In the present paper, binding of melatonin to purified cell nuclei from harderian glands of male and female hamsters was assessed. Binding of 125I‐melatonin to cell nuclei fulfills the criteria for binding to a receptor site. Binding kinetics exhibit properties such as dependence on time and temperature as well as reversibility, saturability, high affinity and specificity. The dissociation constants (KD) and the number of binding sites (Bmax) for the binding of 125I‐melatonin to harderian gland nuclei were 260 ± 56 pm and 12.2 ± 0.8 fmol/mg protein in male glands, and 280 ± 43 pm and 9.8 ± 0.6 fmol/mg protein in female glands, respectively. Competition experiments showed IC50 values for melatonin of 250 ± 45 pm and 290 ± 68 pm in male and female glands, respectively. Other indoleamines such as N‐acetylserotonine and 5‐metoxytryptamine showed IC50 values in the micromolar range, suggesting that the binding sites are specific for melatonin. Hill analyses of the data show nH values of 0.96–0.98, suggesting the existence of a single class of binding sites. These data indicate that specific 125I‐melatonin binding sites exist in the cell nuclei of Harderian glands in male as well as in female hamsters, without significant differences between them. The KD and Bmax values obtained from the binding in both sexes correlates well with the concentration of melatonin described in these respective Harderian glands. It is hypothesized that the nuclear binding sites of melatonin here described could be a physiological melatonin receptor, which may be involved in the genomic‐dependent antioxidant effects of melatonin on hamster Harderian glands elsewhere reported.

show abstract

Ultrastructural study of lamellar and nucleolus‐like bodies in the harderian gland during postnatal development of the hamster (Mesocricetus auratus)

Cited by 14 publications

References 37 publications

Postnatal development of the harderian gland in the Syrian golden hamster (Mesocricetus auratus): A light and electron microscopic study

Postnatal development of the harderian gland in the Syrian golden hamster (Mesocricetus auratus): A light and electron microscopic study

Female syrian hamster harderian gland: Development and effects of high environmental temperature and melatonin injections on histology and porphyrin deposits

Characterization of melatonin high‐affinity binding sites in purified cell nuclei of the hamster (Mesocricetus auratus) harderian gland

Contact Info

Product

Resources

About