Ultrastructure of Kidney Preservation: Varying the Amount of an Effective Osmotic Agent in Isotonic and Hypertonic Preservation Solutions

Coffey, Aline K.; Andrews, Peter M.

doi:10.1016/s0022-5347(17)51362-2

Cited by 7 publications

(11 citation statements)

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“…5E). Consistent with reports that swelling and ultrastructural damage of renal tubule cells are blocked if approximately 50% of the total buffer osmolarity is due to sucrose (Coffey and Andrews, 1983), RGC beading in PBS or 300 mM HEPES is prevented if the sucrose concentration is doubled to 400 mM (results not illustrated). We did not use these formulations routinely because the RGC axons appeared to be compressed axially into accordion-like ripples after immersion of some preparations in these highly hypertonic solutions, perhaps due to linear shrinkage (results not illustrated).…”

Section: Bead Formation In Other Central Neuronssupporting

confidence: 90%

Moniliform deformation of retinal ganglion cells by formaldehyde‐based fixatives

Stradleigh

Greenberg

Partida

et al. 2015

J of Comparative Neurology

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Protocols for characterizing cellular phenotypes commonly use chemical fixatives to preserve anatomical features, mechanically stabilize tissue, and stop physiological responses. Formaldehyde, diluted in either phosphate-buffered saline or phosphate buffer, has been widely used in studies of neurons, especially in conjunction with dyes and antibodies. However, previous studies have reported that these fixatives induce the formation of bead-like varicosities in the dendrites and axons of brain and spinal cord neurons. We report here that these formaldehyde formulations can induce bead formation in the dendrites and axons of adult rat and rabbit retinal ganglion cells, and that retinal ganglion cells differ from hippocampal, cortical, cerebellar, and spinal cord neurons in that bead formation is not blocked by glutamate receptor antagonists, a voltage-gated Na + channel toxin, extracellular Ca 2+ ion exclusion, or temperature shifts. Moreover, we describe a modification of formaldehyde-based fixatives that prevents bead formation in retinal ganglion cells visualized by green fluorescent protein expression and by immunohistochemistry.

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Section: Bead Formation In Other Central Neuronssupporting

confidence: 90%

Moniliform deformation of retinal ganglion cells by formaldehyde‐based fixatives

Stradleigh

Greenberg

Partida

et al. 2015

J of Comparative Neurology

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“…Another simple solution is phosphate-buffered sucrose, developed by Coffey and Andrews in the early 1980s. Phosphate-buffered sucrose was shown to be effective in kidney preservation confirming the hypothesis that high concentrations of impermeant saccharides suppress hypothermic cell swelling (27,78). When UW-CSS became available, a randomized clinical trial comparing EuroCollins with UW-CSS in kidney preservation showed that DGF was significantly lower in the UW-CSS group (23% vs. 33%).…”

Section: Current Cold Storage Solutionsmentioning

confidence: 71%

“…In addition, mannitol has a beneficial effect as a scavenger of reactive oxygen species, and was therefore added in Marshalls, Bretschneider's histidine-tryptophanketoglutarate (HTK), and Celsior solutions. Sucrose (MW 342) is a disaccharide and is used in the renal preservation solution phosphate-buffered sucrose (27,28). Raffinose (MW 504) is the largest one and a trisaccharide.…”

Section: Cell Swellingmentioning

confidence: 99%

Perspectives in Organ Preservation

2007

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Maintaining organ viability after donation until transplantation is critically important for optimal graft function and survival. To date, static cold storage is the most widely used form of preservation in every day clinical practice. Although simple and effective, it is questionable whether this method is able to prevent deterioration of organ quality in the present era with increasing numbers of organs retrieved from older, more marginal, and even non-heart-beating donors. This review describes principles involved in effective preservation and focuses on some basic components and methods of abdominal organ preservation in clinical and experimental transplantation. Concepts and developments to reduce ischemia related injury are discussed, including hypothermic machine perfusion. Despite the fact that hypothermic machine perfusion might be superior to static cold storage preservation, organs are still exposed to hypothermia induced damage. Therefore, recently some groups have pointed at the beneficial effects of normothermic machine perfusion as a new perspective in organ preservation and transplantation.

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“…Thus, it is likely antioxidants, particularly albumin, in allogenic serum improve chondrocyte survival during cold storage. Additionally, as osmotic agents, such as albumin, sucrose, dextrans, and hydroxyethyl starch, improve cold preservation of organs and tissues [3,5,13,19], osmolality changes induced by albumin in allogenic serum might improve the viability of OCT samples.…”

Section: Discussionmentioning

confidence: 99%

Allogenic Serum Improves Cold Preservation of Osteochondral Allografts

Onuma

Urabe

Naruse

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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Background Although several types of culture medium have been used for preservation of osteochondral allografts, the viability of chondrocytes decreases with increasing storage duration. We previously showed the University of Wisconsin solution is more suitable for graft preservation than culture medium. Questions/purposes We determined whether the addition of allogenic serum to University of Wisconsin solution increases chondrocyte survival during prolonged storage of osteochondral allografts. Methods Osteochondral tissue samples harvested from the distal femora of rats were preserved in University of Wisconsin solution supplemented with 0%, 1%, 10%, and 50% allogenic serum at 4°C for 14 days. Cell viability and chondrocyte degenerative changes of the samples then were assessed using a tetrazolium assay and histologic methods. We also evaluated time-dependent changes in cell viability and histologic findings of samples preserved for 7, 14, and 21 days in University of Wisconsin solution supplemented with or without 10% allogenic serum. Results After 14 days of preservation, osteochondral tissue samples maintained in University of Wisconsin solution containing 10% or greater allogenic serum exhibited the highest cell viability and lowest degenerative changes in chondrocytes. In the evaluation of time-dependent changes, we found the chondrocyte degenerative changes were greater in cartilage preserved in University of Wisconsin solution alone than in University of Wisconsin solution containing 10% allogenic serum after Day 7 or later. Conclusions Our results suggest the addition of 10% allogenic serum to University of Wisconsin solution enhances viability of osteochondral tissue samples. Clinical Relevance The use of allogenic serum-supplemented University of Wisconsin solution is expected to prolong the duration of osteochondral allograft storage and result in higher-quality grafts.

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Ultrastructure of Kidney Preservation: Varying the Amount of an Effective Osmotic Agent in Isotonic and Hypertonic Preservation Solutions

Cited by 7 publications

References 0 publications

Moniliform deformation of retinal ganglion cells by formaldehyde‐based fixatives

Moniliform deformation of retinal ganglion cells by formaldehyde‐based fixatives

Perspectives in Organ Preservation

Allogenic Serum Improves Cold Preservation of Osteochondral Allografts

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