Ultrastructure of Shell Gland Tissue from Hens Producing Good and Poor Eggshells

Huntley, D. M.; Holder, D. P.

doi:10.3382/ps.0571365

Cited by 7 publications

(5 citation statements)

References 2 publications

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“…Eggshell constituent ions that participate in eggshell mineralization are transported through uterine fluid. Many studies have reported that the lack of uterine fluid secretion has a negative effect on eggshell formation ( Abe, 1996 ; Huntley and Holder, 1978 ). Hen aging promoted the damage and atrophy of the uterine tissue, which resulted in the lack of secretion of uterine fluid.…”

Section: Discussionmentioning

confidence: 99%

“…The uterus or shell gland histologically consists of pseudo-stratified ciliated columnar epithelial cells, which include ciliated and non-ciliated cells, basal cells, and goblet cells ( Mohammadpour et al, 2012 ). Structural and functional changes of the shell gland directly affect the quality of the eggshell ( Huntley and Holder, 1978 ). The eggshell consists of the eggshell membrane, mammillary layer, palisade layer and cuticle ( Hincke et al, 2012 ; Nys et al, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

Park

Sohn

2018

Korean J Food Sci Anim Resour

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The eggshell, which is a complex and highly ordered structure, is very important factor for food safety and egg marketing. This study investigated the changes in eggshell structure and shell components in relationship to hen age. For this study, we examined the histological change of the endometrium of the 30-, 60-, and 72-wk-old commercial layers, and analyzed the ultrastructure and ionic composition of their eggshells. The results showed that histological deformation, fibrosis, atrophy and elimination of micro-villi in the uterus endometrium were found through microscopic observation that was associated with increasing hen age. Concentration of blood-ion components such as Ca2+, Na+, K+, and Cl– ions did not change with age. Along with the results from the ultrastructure analysis of the eggshell, the palisade layer ratio and the density of mammillary knobs were significantly decreased in older hens. In addition, the type B mammillary knobs were frequently observed with increasing hen age. In the mineral element assay from the eggshell, Ca2+, S2–, and Co2+ significantly decreased with increasing hen age, whereas Na+, K+, and V2+ significantly increased. Therefore, the damages of endometrial tissue inhibit the processes of ion transmission and the crystallization of eggshell formation, resulting in a large and non-uniform mammillary knob formation. This means the conditions of endometrial cells affect the formation of the eggshell structure. In conclusion, hen aging causes the weakness of the eggshell and degrades the eggshell quality.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

Park

Sohn

2018

Korean J Food Sci Anim Resour

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“…In brief, the formation of PE is associated with changes in the uterine glands. Another study reported that the morphological structure of the uterus determines the eggshell quality ( Huntley and Holder, 1978 ). However, the molecular mechanism regulating the formation of pimpled eggshells has rarely been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…The uterine gland cells transfer CO 2 and Ca 2+ to the UF. Endometrium atrophy, which can be a result of an increase in uterus cilia length or decreased secretion of gonadal hormones, can reduce the regenerative capacity of the endometrial cells, declining the function of different ion channels in the uterine gland cells ( Huntley and Holder, 1978 ; Woudstra and Thomson, 2002 ). This may particularly cause abnormal transport of calcium ions, resulting in disordered CaCO 3 deposition and eggshell defects such as speckles, cracks, and pimples ( Zhu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

TMT-based quantitative proteomic analysis unveils uterine fluid difference in hens producing normal and pimpled eggs

Song,

Weng,

Bao

et al. 2023

Poultry Science

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“…특히 난각을 형성하는 자궁의 조직학적 구조는 주름진 위 중층 원주상피 세포로 기저세포와 배상세포로 구성된다 (Mohammadpour et al, 2012). 이러한 자궁 표면의 세포구성은 난각질과 밀접한 관련을 가지며, 환우 때에 산란이 중지되면 자궁내막의 상 피세포가 탈락되고, 점막 고유층의 밀도가 감소되어 점막세 포의 소실이 일어난다 (Huntley and Holder, 1978;Ariyadi et al, 2012). 난각의 구성은 95%의 calcium carbonate와 5%의 유기물질로 된 얇은 보호막으로, 복잡하고 고도화 된 다층 석회화 구조이다 (Romanoff, 1949;Tyler, 1969).…”

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Histological Change of Uterus Endometrium and Expression of the Eggshell-related Genes according to Hen Age

Park¹,

Cho²,

Park³

et al. 2017

Korean Journal of Poultry Science

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The eggshell is an intricate and highly ordered structure composed of multiple layers and a calcified matrix. The eggshell is formed at the uterine segment of the chicken oviduct. In this study, histological changes in the uterine endometrium and the expression of the eggshell-related genes were investigated according to hen age. We analyzed the expression of eggshell protein-related genes, such as OCX-32, OCX-36, OC-17, OC-116, and eggshell-ion-related genes, such as CABL-1, SPP1, SCNN1G, ATP2A2, CA2, and CALM1. In chicken uterine endometrium, histological deformation, fibrosis, atrophy and elimination of micro-villi were found with increasing hen age. The concentration of blood-ion components did not significantly change with age. The amount of telomeric DNA in uterine endometrial cells decreased with increasing hen age. The expression of most of the eggshell-related genes changed significantly with increasing hen age. The expression of some ovo-proteins, which play a role in eggshell formation, increased with increasing hen age; however, there were no significant correlations among eggshell protein genes. Eggshell ion-related genes, such as ATP2A2, SCNN1G, CA2, and CALM1, were closely related to each other. The OCX-32 and OCX-36 genes were closely related to some of the eggshell ion genes. Eggshell protein-related genes, such as the OCX-32, OCX-36 genes and ion-related genes such as CALB-1, ATP2A2, SCNN1G, CA2, CALM1, affected eggshell formation, mutually or independently. This study shows that, uterine although endometrial cell damage occurs with increasing hen age, normal eggshells can be formed in old hens. This suggests that eggshell protein-and eggshell ion-related genes also control the homeostasis of eggshell formation.

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Ultrastructure of Shell Gland Tissue from Hens Producing Good and Poor Eggshells

Cited by 7 publications

References 2 publications

The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

The Influence of Hen Aging on Eggshell Ultrastructure and Shell Mineral Components

TMT-based quantitative proteomic analysis unveils uterine fluid difference in hens producing normal and pimpled eggs

Histological Change of Uterus Endometrium and Expression of the Eggshell-related Genes according to Hen Age

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