Ultrastructure of the fresh water cyanobacterium Anabaena variabilis SPU 003 and its application for oxygen-free hydrogen production

Shah, V

doi:10.1016/s0378-1097(00)00508-5

Cited by 7 publications

(11 citation statements)

References 13 publications

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“…The results of this study are different from that obtained by Shah et al (2001), who showed a decline in hydrogen production by cyanobacteria in the presence of dyes. Variable results also have been reported on growth and photosynthesis of green algae and cyanobacteria with respect to heavy metals (Whitton 1970;Takamura et al 1989;Horcsik et al 2006).…”

Section: Tolerance Studiescontrasting

confidence: 99%

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Screening metal-dye-tolerant photoautotrophic microbes from textile wastewaters for biohydrogen production

Mona

Kaushik

2014

J Appl Phycol

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In the present study, tolerance of ten microalgal strains isolated from wastewaters of different textile mills in relation to two metals and dyes was studied based on cell growth estimated spectrophotometrically. Three cyanobacterial strains that were found to tolerate both Cr(VI) and Co(II) along with the dyes reactive red 198 (RR 198) and crystal violet (CV) were investigated further for the concentration of various photosynthetic pigments and exopolymer production in the presence of the dyes and metals. All three tolerant species-Nostoc linckia HA-46, Myxosarcina spectabilis HP-43 and Gloeocapsa calcarea HP-45-showed a significantly higher concentration (P<0.05) of various pigments when the medium was spiked with metals or dyes. Production of extracellular proteins and particularly extracellular polysaccharides by the tolerant strains increased significantly (P<0.05) in the presence of metals. The effect of dyes was, however, not always statistically significant (P>0.05). Production of hydrogen by these photoautotrophic microbes was moderate (19-28 nmol h −1 mg −1 dry wt). The best performing strain, N. linckia, when examined further for its hydrogen production potential in the presence of the two dyes and metals, showed significantly higher rates of hydrogen production in the presence of Cr, Co and RR 198. Its hydrogenase activity also followed the same trend. Immobilization of the microbe into alginate beads almost doubled the hydrogen production by the organism in the control as well as in the presence of suitable concentrations of the two metals (10 mg L −1 ) and dyes (50 mg L −1 ).

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Section: Tolerance Studiescontrasting

confidence: 99%

“…Some earlier studies on cyanobacteria, however, reported reduced hydrogen production in the presence of dyes (e.g. Shah et al 2001). …”

Section: Tolerance Studiesmentioning

confidence: 93%

Screening metal-dye-tolerant photoautotrophic microbes from textile wastewaters for biohydrogen production

Mona

Kaushik

2014

J Appl Phycol

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Section: Rates Of Cyanobacterial Hydrogen Productionmentioning

confidence: 99%

“…Interestingly, nitrogen-fixing cells of A. variabilis SPU 003 have the capacity to produce hydrogen mainly in darkness (181). Addition of various sugars stimulated the production of hydrogen, with mannose giving the highest rate, 5.58 nmol of hydrogen produced per mg dry weight per h (181). Since the hydrogen production is the result of hydrogen evolution catalyzed by nitrogenase and a hydrogen consumption catalyzed mainly by an uptake hydrogenase, the obvious improvements are to increase the hydrogen production by using alternative nitrogenases and by inhibiting the activity of the uptake hydrogenase.…”

Section: Rates Of Cyanobacterial Hydrogen Productionmentioning

confidence: 99%

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Tamagnini

Axelsson

Lindberg

et al. 2002

Microbiol Mol Biol Rev

466

372

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Cyanobacteria may possess several enzymes that are directly involved in dihydrogen metabolism: nitrogenase(s) catalyzing the production of hydrogen concomitantly with the reduction of dinitrogen to ammonia, an uptake hydrogenase (encoded by hupSL) catalyzing the consumption of hydrogen produced by the nitrogenase, and a bidirectional hydrogenase (encoded by hoxFUYH) which has the capacity to both take up and produce hydrogen. This review summarizes our knowledge about cyanobacterial hydrogenases, focusing on recent progress since the first molecular information was published in 1995. It presents the molecular knowledge about cyanobacterial hupSL and hoxFUYH, their corresponding gene products, and their accessory genes before finishing with an applied aspect—the use of cyanobacteria in a biological, renewable production of the future energy carrier molecular hydrogen. In addition to scientific publications, information from three cyanobacterial genomes, the unicellular Synechocystis strain PCC 6803 and the filamentous heterocystous Anabaena strain PCC 7120 and Nostoc punctiforme (PCC 73102/ATCC 29133) is included

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“…The cyanobacterium Anabaena variabilis has been studied extensively for its high potential of H 2 production and N 2 fixation and is regarded as potential candidate for future development of biologically produced clean energy, H 2 (Borodin et al 2000;Vyas and Kumar 1995;Shah et al 2001). It is also important in global carbon sequestration via photosynthetic CO 2 fixation (Nyhus et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Studies on the factors affecting the growth and hemolytic activity of Anabaena variabilis

Wang

Xiu-kai

2007

J Appl Phycol

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The hemolytic activity of the cell-free culture supernatant of Anabaena variabilis OL S1 was investigated using the hemolysis of rabbit erythrocytes as an assay. The culture medium of A. variabilis started to exhibit hemolytic activity at the late exponential growth phase, and maximized at the stationary phase. The hemolytic toxin is heat-stable and can be extracted in dichloromethane. The hemolytic activities under different temperature, light intensity and pH showed a high correlation with the cell densities (r = 0.965, 0.951, 0.865, respectively), and the optimum condition is 28~30°C, pH 7.5~8.0, light intensity 120 μmol photons m −2 s −1 . The addition of 10~20 μg mL −1 chloramphenicol, an inhibitor of protein synthesis, exhibited no marked suppression on the hemolytic activity. The supplement of 1~20 μg mL −1 glycerol increased the hemolytic activity significantly, suggesting that synthesis of hemolysin was dependent on carbohydrate and lipid metabolism. The spectrum of erythrocyte sensitivity to the hemolysin indicated that rabbit erythrocytes were more sensitive to the hemolysin than were rat and human erythrocytes. Goldfish and cat erythrocytes were, however, insensitive to the hemolytic toxin of A. variabilis.

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Ultrastructure of the fresh water cyanobacterium Anabaena variabilis SPU 003 and its application for oxygen-free hydrogen production

Cited by 7 publications

References 13 publications

Screening metal-dye-tolerant photoautotrophic microbes from textile wastewaters for biohydrogen production

Screening metal-dye-tolerant photoautotrophic microbes from textile wastewaters for biohydrogen production

Hydrogenases and Hydrogen Metabolism of Cyanobacteria

Studies on the factors affecting the growth and hemolytic activity of Anabaena variabilis

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