Ultrastructure of the Primary Immune Response

Neher, Galen H.; Siegel, B

doi:10.1159/000230342

Cited by 10 publications

(1 citation statement)

References 22 publications

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“…In ultrastructural observations of plaque-forming cells (PFC) Harris et al [6], Neher and Siege1 [7] and Mazie [8] demonstrated that cells from both lymphocptic and plasmacytic series are active units in the antibody-secreting process. Using immunocytological techniques, a similar heterogeneity was observed in AFC [9-121. The purpose of this work was t o identify the antibody-secreting cells and the ultrastructural localization of antibody molecules by combining appropriate techniques.…”

Section: Introductionmentioning

confidence: 99%

The relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti‐peroxidase anti bodies in plaque‐forming cells of the rabbit popliteal lymph node

et al. 1976

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The ultrastructure of antibody-forming cells (AFC) has been studied in the lymph node cell population from rabbits locally immunized with horseradish peroxydase (PO) incorporated in complete Freund's adjuvant, and the kinetics of AFC development followed from day 7 to day 18 after one injection of PO. Identification of the AFC was done by local hemolysis assay, using carboxymethyl cellulose solidifying medium and PO-coated sheep erythrocytes. AFC were thereafter transferred by micromanipulation into a Beem capsule, fixed, treated by PO for fine ultrastructure detection of anti-PO antibodies, included, sectioned and studied by electron microscopy. It was found that the AFC were essentially of three categories: lymphocytes, proplasmacytes and plasmacytes, with (+), or without (-), intracellular antibody. The proportion of these categories varies with the time elapsed since the injection of antigen and with the plaque-forming activity of the population: lymphocytes (-) are relatively more numerous (over 30%) at the early stages of immunization (day 7). The number of plasmacytes increases with immunization. If most of them contain intracellular antibody at the early stages (up to day 9), the proportion of plasmacytes (+) decreases markedly afterwards. The cell type distribution is compatible with the idea that the lymphocytes are the precursors of plasmacytes, proplasmacytes being transitional forms, but no direct filiation scheme can actually be deduced from these experiments.

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Section: Introductionmentioning

confidence: 99%

The relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti‐peroxidase anti bodies in plaque‐forming cells of the rabbit popliteal lymph node

et al. 1976

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Ultrastructural analysis of normal and immunized spleen of the snapping turtle, Chelydra serpentine

Borysenko

1976

Journal of Morphology

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In the ultrastructural comparison of normal, unimmunized spleens with immunized spleens at key intervals after antigenic stimulation with keyhole limpet hemocyanin (KLH), we noted cellular and cytological features which reflect the cellular kinetics of the primary immune response, particularly with respect to plasma cell production. Although lymphoblasts and mature plasma cells are present in the white and red pulp, respectively, intermediate stages of the plasma cell line are rarely found in normal spleen. Following antigenic challenge, we found a marked increase in lymphoblasts in the white pulp, most of them containing short segments of rough endoplasmic reticulum suggesting initial differentiation toward plasma cells. Following an apparent migration of cells from the white to the red pulp, we found plasma cells in various stages of maturation in the red pulp cords and sinuses. The ultrastructural features of these cells reflect the differentiation of lymphoblasts into mature plasma cells. Both immature and mature plasma cells usually possess dilated cisternae of rough endoplasmic reticulum, suggesting that they are capable of producing and storing a secretory product, presumably antibody. We also noted a large number of immature macrophages and monocytes in immunized spleens. These cellular events and their cytological characteristics are compared to those described in other vertebrate classes.

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Cellular Aspects of Humoral Immune Responsiveness in Chelydra

Borysenko

1975

Advances in Experimental Medicine and Biology

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Ultrastructure of the Primary Immune Response

Cited by 10 publications

References 22 publications

The relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti‐peroxidase anti bodies in plaque‐forming cells of the rabbit popliteal lymph node

The relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti‐peroxidase anti bodies in plaque‐forming cells of the rabbit popliteal lymph node

Ultrastructural analysis of normal and immunized spleen of the snapping turtle, Chelydra serpentine

Cellular Aspects of Humoral Immune Responsiveness in Chelydra

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