Ultrastructure study of Vanda Kasem's Delight orchid's protocorm-like body

Gnasekaran, Pavallekoodi; Mahmood, Maziah; Subramaniam, Sreeramanan

doi:10.1590/s0102-05362016003005

Cited by 10 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ornamental plant market, which includes orchids, covered 8% of the world floriculture trade, considered an international business (GNASEKARAN et al, 2016). This growth may be associated with the inexistence of specific criteria, regardless of academic level or gender specificity to cultivate these plants.…”

Section: Resultsmentioning

confidence: 99%

Orchidists’ profile, management and perception of the main pests in orchid cultivation

et al. 2022

View full text Add to dashboard Cite

This study investigated the profile of orchidists, their cultivation practices, and their knowledge about pests in orchid cultivation in the state of Bahia. There were applied 74 questionnaires to producers (amateurs and commercials) in 23 municipalities. The data were submitted to univariate statistics using the chi-square test, the Mann-Whitney U-test and the Kruskal-Wallis H-test. The survey shows that orchid cultivation is mostly practiced by women (67.6%) and those with higher education (48.7%). Most respondents were amateurs (87.8%). Insects (39.9%) and diseases (32.5%) were the main organisms associated with phytosanitary problems. The presence of insects was reported to occur mainly in the leaves (47%), mainly mealybugs (46.3%). Correspondence was significant between commercial and amateur groups according to the pairwise Mann-Whitney U-test (1) = 370.04 and p < 0.01. The Kruskal-Wallis H-test (27) = 924.78 and p < 0.01 discriminated categories between the groups. Despite the growing interest in orchid cultivation in the state of Bahia, most orchidists are amateurs. The study warns about the risk of pest dispersion between crops since exchanging plants and purchasing specimens in open markets are common practices.

show abstract

Section: Resultsmentioning

confidence: 99%

Orchidists’ profile, management and perception of the main pests in orchid cultivation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Histological steps established by Gnasekaran et al (2016) were adapted to study anatomical features of primary and sub-cultured callus. Induced and sub-cultured (4-weeks-old) callus were fixed in formalin-acetic acid-ethanol (FAA) fixative for 4 days and rinsed with fresh tap water at 1 hour interval for 6 hours on day 5.…”

Section: Histological Analysis Of Primary and Subcultured Callusmentioning

confidence: 99%

Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

Gnasekaran

Rahman

Chew

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Plant cells are driven by types and concentrations of plant growth regulators to produce callus mass containing bioactive compounds. This study aimed to induce callus and to observe the histological, phytochemicals, and antioxidant basis of the callus. An efficient callus induction protocol was developed using picloram for Malaysian red ginger, Zingiber officinale var. rubrum. The effect of auxinic picloram herbicide was studied using six different concentrations (0, 0.5, 1, 2, 4, and 8 mg/L) on various explants (leaf sheath, leaf, root) to optimise the callus induction. The induced callus was studied for growth kinetics, anatomical features, antioxidant capacity, and phytochemical content. The highest callogenesis frequency (93.75%) and biomass accumulation (3.68 g) were observed on leaf sheath explant cultured on ½ strength Murashige and Skoog (MS) medium supplemented with 8 mg/L which also requires earlier subculture duration (45 days post-inoculation) in comparison to lower concentrations. Morphological investigation through histological procedure demonstrated friable and non-embryogenic characteristics of the primary and subcultured callus. Cultivated leaf sheath (CLS) methanolic extract showed the highest total phenolic (191.26 mg GAE/g dry extract) and flavonoid (4.54 mg QE/g dry extract) contents contributing to antioxidant activity with an estimated EC50 of 0.208 mg/mL. Although comparatively lower than CLS extract, callus extracts showed higher antioxidant activity and significantly lower EC50 values than in vitro leaf sheath extract. 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, phenol, and phenolic glucoside were only present in callus cultures while methyl esters, fatty acids, and phytosterols could be obtained from leaf sheath and callus extracts. In conclusion, the callus culture of Z. officinale var. rubrum is a potential renewable source of bioactive phytochemical compounds and can be employed for biotechnological practices such as elicitor-induced accumulation of secondary metabolites and genetic modification.

show abstract

“…For histology and SEM analyses, protocols developed by Gnasekara et al (2016) was applied for this study.…”

Section: Histology and Scanning Electron Microscopy Analyses (Sem)mentioning

confidence: 99%

Thidiazuron (TDZ) induced organogenesis and clonal fidelity studies in Haloxylon persicum (Bunge ex Boiss & Buhse): an endangered desert tree species

Kurup

Purayil

Alkhaili

et al. 2018

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

(Bunge ex Boiss & Buhse), is one of the hardy woody desert shrubs, which is now endangered and/or nearing extinction. Urban landscape development and overgrazing are the major threats for the erosion of this important plant species. For conserving the species, it is critical to develop an efficient in vitro regeneration protocol for rapid multiplication of large number of regenerants. Leaf explants, cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) (0.5, 1, 2 µM), showed significant difference in bud sprouting and adventitious shoot induction. The highest shoot bud formation was recorded on MS medium supplemented with 0.5 µM TDZ. Shoot tip necrosis (STN), observed after first subculture of shoot buds in same medium, increased in severity with subculture time. Application of calcium (4 mM) and boron (0.1 mM) in combination with kinetin (10 µM) in the subculture medium significantly reduced the intensity of STN. On an average eight shoots/explant were produced by alleviating this problem. ISSR marker analysis revealed monomorphic banding pattern between progenies and parents, indicating the true to type nature of the clones and its parents.

show abstract

Ultrastructure study of Vanda Kasem's Delight orchid's protocorm-like body

Cited by 10 publications

References 11 publications

Orchidists’ profile, management and perception of the main pests in orchid cultivation

Orchidists’ profile, management and perception of the main pests in orchid cultivation

Picloram enhanced the callus induction, growth kinetics, antioxidant potentials, and secondary metabolites production of Zingiber officinale var. rubrum callus cultures

Thidiazuron (TDZ) induced organogenesis and clonal fidelity studies in Haloxylon persicum (Bunge ex Boiss & Buhse): an endangered desert tree species

Contact Info

Product

Resources

About