Ultraviolet and visible spectroscopies for tissue diagnostics: fluorescence spectroscopy and elastic-scattering spectroscopy

Bigio, Irving J.; Mourant, Judith R.

doi:10.1088/0031-9155/42/5/005

Cited by 337 publications

(215 citation statements)

References 78 publications

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“…Many types of probes have been used within the research community and cannot all be summarized here; however, comprehensive reviews of DRS, LIFS, and RS probe technology are available. 34 Briefly, combination LIFS-DRS probes have been demonstrated by past studies, 35,36 as well as a probe combining all three techniques. 32 There are several important, and challenging, design and functional considerations for a probe that combines reflectance, fluorescence, and Raman signals.…”

Section: Fiber-optic Probesmentioning

confidence: 98%

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Sharma

Marple²,

Reichenberg

et al. 2014

Review of Scientific Instruments

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The design and characterization of an instrument combining Raman, fluorescence, and reflectance spectroscopic modalities is presented. Instrument development has targeted skin cancer applications as a novel fiber-optic probe has been specially designed to interrogate cutaneous lesions. The instrument is modular and both its software and hardware components are described in depth. Characterization of the fiber-optic probe is also presented, which details the probe's ability to measure diagnostically important parameters such as intrinsic fluorescence and absorption and reduced scattering coefficients along with critical performance metrics such as high Raman signal-to-noise ratios at clinically practical exposure times. Validation results using liquid phantoms show that the probe and system can extract absorption and scattering coefficients with less than 10% error. As the goal is to use the instrument for the clinical early detection of skin cancer, preliminary clinical data are also presented, which indicates our system's ability to measure physiological quantities such as relative collagen and nicotinamide adenine dinucleotide concentration, oxygen saturation, blood volume fraction, and mean vessel diameter.

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Section: Fiber-optic Probesmentioning

confidence: 98%

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Sharma

Marple²,

Reichenberg

et al. 2014

Review of Scientific Instruments

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show abstract

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] This technology is cost-effective, and can be deployed through fiber optic probes to quickly, nondestructively, and quantitatively detect the structural, physiological, and biochemical changes that are associated with the progression of disease. Several studies have demonstrated that there are significant differences in the fluorescence [1][2][3]5,7,9,11 and diffuse reflectance 4,13-17 spectra of normal, benign, and malignant breast tissues, which could be used for the diagnosis of breast malignancy.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of breast cancer using fluorescence and diffuse reflectance spectroscopy: a Monte-Carlo-model-based approach

et al. 2008

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We explore the use of Monte-Carlo-model-based approaches for the analysis of fluorescence and diffuse reflectance spectra measured ex vivo from breast tissues. These models are used to extract the absorption, scattering, and fluorescence properties of malignant and nonmalignant tissues and to diagnose breast cancer based on these intrinsic tissue properties. Absorption and scattering properties, including β-carotene concentration, total hemoglobin concentration, hemoglobin saturation, and the mean reduced scattering coefficient are derived from diffuse reflectance spectra using a previously developed Monte Carlo model of diffuse reflectance. A Monte Carlo model of fluorescence described in an earlier manuscript was employed to retrieve the intrinsic fluorescence spectra. The intrinsic fluorescence spectra were decomposed into several contributing components, which we attribute to endogenous fluorophores that may present in breast tissues including collagen, NADH, and retinol/ vitamin A. The model-based approaches removes any dependency on the instrument and probe geometry. The relative fluorescence contributions of individual fluorescing components, as well as β-carotene concentration, hemoglobin saturation, and the mean reduced scattering coefficient display statistically significant differences between malignant and adipose breast tissues. The hemoglobin saturation and the reduced scattering coefficient display statistically significant differences between malignant and fibrous/benign breast tissues. A linear support vector machine classification using (1) fluorescence properties alone, (2) absorption and scattering properties alone, and (3) the combination of all tissue properties achieves comparable classification accuracies of 81 to 84% in sensitivity and 75 to 89% in specificity for discriminating malignant from nonmalignant breast tissues, suggesting each set of tissue properties are diagnostically useful for the discrimination of breast malignancy.

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“…Elastic scattering spectroscopy (ESS) is an emerging technique for generating a wavelength spectrum that reflects structural and morphological changes within tissues (Bigio and Mourant 1997;Green et al 2014). ESS has been shown to be sensitive to nuclear size, chromatin content, and nuclear/cytoplasmic ratio-all of which characteristics are of interest to the histopathologist who is screening for malignancy within tissue (Wallace and Van Dam 2000).…”

Section: Elastic Scattering Spectroscopymentioning

confidence: 99%

Non-Invasive Techniques for Detection and Diagnosis of Oral Potentially Malignant Disorders

Liu

Zhao

Zeng

et al. 2016

Tohoku J. Exp. Med.

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Oral squamous cell carcinoma (OSCC) is the most common oral and maxillofacial malignancy, and its morbidity and mortality rates are still high in most countries. Oral potentially malignant disorders (PMDs) are used to refer to a heterogeneous group of conditions that are characterized by increased risk for malignant transformation to OSCC. Currently identified oral PMDs include leukoplakia, erythroplakia, palatal lesions associated with reverse smoking, oral lichen planus, oral submucous fibrosis, actinic keratosis, and discoid lupus erythematosus. The early detection and diagnosis of these lesions are important for cancer prevention and disease management. In recent years, there has been a growing and persistent demand for new non-invasive, practical diagnostic techniques that might facilitate the early detection of oral PMDs. The non-invasive detection techniques evaluated in this review are divided into four categories: vital staining with a solution that can be used as a mouth rinse or applied onto a suspected area of the mouth, light-based detection systems, optical diagnostic technologies that employ returned optical signals to reflect structural and morphological changes within tissues, and salivary biomarkers. Most of these techniques have shown great potential for screening and monitoring oral PMDs. In this review article, the authors critically assess these non-invasive detection techniques for oral PMDs. We also provide a summary of the sensitivity and specificity of each technique in detecting oral PMDs and oral cancer, as well as their advantages, disadvantages, clinical applications, and indications.

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Ultraviolet and visible spectroscopies for tissue diagnostics: fluorescence spectroscopy and elastic-scattering spectroscopy

Abstract: We review the application of fluorescence spectroscopy and elastic-scattering spectroscopy, over the ultraviolet-to-visible wavelength range, to minimally invasive medical diagnostics. The promises and hopes, as well as the difficulties, of these developing techniques are discussed.

Cited by 337 publications

References 78 publications

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Design and characterization of a novel multimodal fiber-optic probe and spectroscopy system for skin cancer applications

Diagnosis of breast cancer using fluorescence and diffuse reflectance spectroscopy: a Monte-Carlo-model-based approach

Non-Invasive Techniques for Detection and Diagnosis of Oral Potentially Malignant Disorders

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