Umbilical cord blood collection before placental delivery during cesarean delivery increases cord blood volume and nucleated cell number available for transplantation

Surbek, Daniel; Visca, E.; Steinmann, Christian; Thewis, André; Schatt, Stefan; Hahn, Sinuhe; Gratwohl, Aloïs; Holzgreve, Wolfgang

doi:10.1067/mob.2000.105744

Cited by 40 publications

(52 citation statements)

References 16 publications

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“…While in the first case collection is performed immediately after cord blood clamping, in the second case it is necessary to wait till placental expulsion. Other authors have confirmed better results in the samples collected before placental expulsion for caesarean deliveries too [25]. …”

Section: Discussionsupporting

confidence: 60%

Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units

Solves¹,

Perales

Moraga

et al. 2005

Acta Haematol

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Background and Objectives: Umbilical cord blood (UCB) contains haematopoietic stem cells and can be used as an alternative to bone marrow transplantation in certain cases. Engraftment was dependent upon the haematopoietic progenitor cell content of the cord blood units. This study was designed to investigate the influence of obstetric, neonatal and collection factors on the volume and haematopoietic content of UCB donations. Material and Methods: A retrospective analysis of obstetric and neonatal factors was performed from 300 cord blood donations in Valencia Cord Blood Bank. Maternal, neonatal and collection factors influencing cord blood quality measured as volume, total nucleated cell count, CD34+ cells and colony-forming units (CFU) were analysed. Results: Bigger babies produced cord blood units with larger volume, higher cells counts, CFU and CD34 cell counts. In the multivariate analysis, we found that both placental weight and mode of collection were predictor variables for total nucleated cell count, CD34 cells and CFU. Conclusion: Our study concludes that cord blood units must be collected before placental delivery and that birth weight, as an estimation of the placental weight, could be added to standard cord blood donors criteria in order to improve the bank efficiency.

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Section: Discussionsupporting

confidence: 60%

Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units

Solves¹,

Perales

Moraga

et al. 2005

Acta Haematol

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“…A similar finding was also reported for in utero versus ex utero CB collection following cesarean section delivery. 9 Our comparison of CB units collected following vaginal delivery with those collected following cesarean section delivery revealed significant differences in the WBC concentration and volume collected. Yamada et al 10 also reported a higher CB volume following cesarean section delivery (with ex utero collection) compared with vaginal delivery (with in utero collection).…”

Section: Discussionmentioning

confidence: 79%

Influence of mode of birth and collection on WBC yields of umbilical cord blood units

et al. 2002

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“…This result is 39%-75% higher than UCB units collected by standard methods at several operative banks [24-26, 30-35, 58]. Other studies have tried to identify the optimal UCB collection techniques assessing the influence of mode of delivery (vaginal vs. cesarean) and harvest timing (before vs. after placental delivery) [31,[59][60][61][62][63]. Despite improved cell recovery claimed for certain procedures, the NC yields did not exceed the numbers reported by UCB banks [24-26, 30-35, 58].…”

Section: Discussionmentioning

confidence: 99%

A Modified Cord Blood Collection Method Achieves Sufficient Cell Levels for Transplantation in Most Adult Patients

et al. 2005

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Umbilical cord blood transplantation (UCBT) has been used increasingly in both pediatric and adult patients. The total nucleated cell (NC) dose infused is the most critical factor in determining speed of engraftment and survival. Using standard collection techniques, the mean NC content of UCB units is about 10 × 10 8, and only 25% of these units reach the target cell dose of 2 × 10 7 /kg in UCBT patients weighing 50-70 kg. We have designed a modified placental/umbilical two-step collection method in which a standard blood fraction obtained by umbilical venipuncture is combined with a second fraction harvested after placental perfusion with 50 ml heparinized 0.9% saline. This second fraction contributed 32% volume and 15% NCs to the whole UCB unit (123.7 ± 50.1 ml and 1.26 ± 0.52 × 10 9 NC). The proportion of progenitor cells in both fractions was not significantly different, indicating that the hematopoietic potential of these larger units is 20% (range, 2%-100%) higher than UCB units collected by standard methods. In addition, the bacterial contamination rate associated with this novel collection method (2.78%) compares favorably. Since 1998 we have further enriched our units by processing only UCB units over 0.8 × 10 9 NCs, resulting in a 36% cell increment (1.46 ± 0.52 × 10 9 NCs). Thus, 84% and 54% of the Madrid UCB Bank inventory would fulfill the target cell dose of 2 × 10 7 /kg in patients weighing 50 and 65 kg, respectively. This significant UCB banking improvement gives larger pediatric and adult patients a greater chance of finding adequate grafts in order to achieve better clinical outcomes after UCBT. Stem Cells 2005;23:324-334

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Umbilical cord blood collection before placental delivery during cesarean delivery increases cord blood volume and nucleated cell number available for transplantation

Cited by 40 publications

References 16 publications

Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units

Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units

Influence of mode of birth and collection on WBC yields of umbilical cord blood units

A Modified Cord Blood Collection Method Achieves Sufficient Cell Levels for Transplantation in Most Adult Patients

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