Un programme social pour la lutte physique contre la leishmaniose cutanée zoonotique dans la wilaya de M'Sila en Algérie

Cherif, K.; Boudrissa, A.; Cherif, Mokhtar Hamdi; Harrat, Zoubir

doi:10.3917/spub.126.0511

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…However, in the field, distinct phlebotomine species could live in sympatry, some species could be vectors and others, non-vectors of Leishmania spp. To confirm that MALDI-TOF MS could be useful for species identification of field-caught specimens, phlebotomine sand flies caught inside animal shelters from four locations in Algeria, where human and canine leishmaniasis cases have been reported [ 31 , 42 ] were tested in the present study.…”

Section: Discussionmentioning

confidence: 99%

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Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

et al. 2016

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BackgroundPhlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. Precise sand fly identification is essential to prevent phlebotomine-borne diseases. Over the past two decades, progress in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an accurate tool for arthropod identification. The objective of the present study was to investigate the usefulness of MALDI-TOF MS as a tool for identifying field-caught phlebotomine.Methodology/Principal FindingsSand flies were captured in four sites in north Algeria. A subset was morphologically and genetically identified. Six species were found in these areas and a total of 28 stored frozen specimens were used for the creation of the reference spectrum database. The relevance of this original method for sand fly identification was validated by two successive blind tests including the morphological identification of 80 new specimens which were stored at -80°C, and 292 unknown specimens, including engorged specimens, which were preserved under different conditions. Intra-species reproducibility and inter-species specificity of the protein profiles were obtained, allowing us to distinguish specimens at the gender level. Querying of the sand fly database using the MS spectra from the blind test groups revealed concordant results between morphological and MALDI-TOF MS identification. However, MS identification results were less efficient for specimens which were engorged or stored in alcohol. Identification of 362 phlebotomine sand flies, captured at four Algerian sites, by MALDI-TOF MS, revealed that the subgenus Larroussius was predominant at all the study sites, except for in M’sila where P. (Phlebotomus) papatasi was the only sand fly species detected.ConclusionThe present study highlights the application of MALDI-TOF MS for monitoring sand fly fauna captured in the field. The low cost, reliability and rapidity of MALDI-TOF MS analyses opens up new ways in the management of phlebotomine sand fly-borne diseases.

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Section: Discussionmentioning

confidence: 99%

“…P . papatasi was also the dominant species captured during an entomological survey of phlebotomine sand flies and control of CL in M’sila [ 42 ]. This species is known as a vector of L .…”

Section: Discussionmentioning

confidence: 99%

Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

et al. 2016

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“…The main reservoir of the disease in Algeria being P. obesus , a pilot project aiming to remove Chenopodiaceae around houses within a radius of 300 m before the transmission season was undertaken in the Msila region in 2003. Over 3,600 ha were treated and the number of cases decreased from 1391 in 2003 to 965 in 2004 [27]. On the other hand, insecticides (Deltamethrin) were used in Algeria on a large scale from 2006 in several governorates, which has led to a significant decline in the incidence rate since 2007 [44].…”

Section: Epidemiological Situation and Control Measures In Na Countriesmentioning

confidence: 99%

Cutaneous Leishmaniasis in North Africa: a review

2014

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In North African countries, cutaneous leishmaniasis transmission has been increasing since the 1980s, with a significant increase in the incidence of cases and a spread of the geographical distribution. The disease currently represents a major public health problem with a productivity gap and an impediment for development, which results in dramatic socioeconomic and psycho-sanitary impacts. The incidence is more than thousands of cases every year in Algeria, Libya, Morocco, and Tunisia. In Egypt, only a few dozen cases per year are reported, mainly in the Sinai Peninsula. Three Leishmania species, associated with distinct eco-epidemiological and clinical patterns, are involved, namely Leishmania infantum, L. major, and L. tropica. However, L. major is by far the most frequent in Algeria, Libya, and Tunisia, with more than 90% of the registered cases. It is mainly encountered in rural areas under semi-arid, arid and Saharan climates. Leishmania tropica is more prevalent in Morocco, reaching 30–40% of isolates in some districts. Much data is still missing concerning the risk factors of the infection and the lesion development, as well as vector and reservoir ecology and behavior. The knowledge of such parameters, following multidisciplinary and integrated approaches, is crucial for better management and control of the disease, that also faces a lack of resources and efficient control measures.

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“…Some of these species are responsible for health problems for humans and domestic animals because they act as reservoirs of various pathogens, which they transmit directly or indirectly to humans such as cutaneous leishmaniasis in several regions in Algeria Cherif et al 2012). Bitam et al (2008) detected the species Bartonella spp in different rodents, this bacterium is the causative agent of Bartonellosis disease.…”

Section: Introductionmentioning

confidence: 99%

Inventory and distribution of the rodents in Aurès Mountains and Ziban oasis (Northeast of Algeria)

Drouai¹,

Belhamra²,

Mimeche³

2018

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Nuestro estudio muestra un inventario de roedores del noroeste de Argelia. Tas especies se capturaron en dos regiones: Taouziant (Montañas Aurès ) y Bouchagroune (oasis de Ziban). El periodo de muestreo duró diez mees en cada lugar: en Taouziant de febrero a nobiembre de 2014 y en Bouchagroune de septiembre de 2013 a junio de 2014. El método de trampeo en línea fue llevado a cabo mediante 40 jaulas-trampas instaladas en las regiones de estudio. Se capturaron ocho especies, que pertenecen a tres subfamilias y seis géneros. Tres especies se distribuyen en las dos regiones Rattus rattus, Mus musculus y Gerbillus amoenus. Tres especies se capturaron en los campos de cereal de Taouziant ( Rattus norvegicus, Meriones shawii y Jaculus jaculus) y dos especies en el palmeral de Bouchagroune (Psammomys obesus y Gerbillus gerbillus) Our study presents an inventory of rodents in Northeast of Algeria. The species were captured at two regions: Taouziant (Aurès Montain) and Bouchagroune (Ziban oasis). The sampling period takes ten months at each site: in Taouziant between February to November 2014 and in Bouchagroune, from September 2013 to June 2014. The method of trapping online was performed using 40 wire traps installed at the studied regions. Eight species were captured. They belong to three subfamilies and six genera. Three species occur in the two regions: Rattus rattus, Mus musculus and Gerbillus amoenus. Three species were captured in Taouziant cereal fields ( Rattus norvegicus, Meriones shawii and Jaculus jaculus) and two species were found in Bouchagroune palm grove (Psammomys obesus and Gerbillus gerbillus)

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Un programme social pour la lutte physique contre la leishmaniose cutanée zoonotique dans la wilaya de M'Sila en Algérie

Cited by 10 publications

References 14 publications

Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

Cutaneous Leishmaniasis in North Africa: a review

Inventory and distribution of the rodents in Aurès Mountains and Ziban oasis (Northeast of Algeria)

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