Unamplified and Real‐Time Label‐Free miRNA‐21 Detection Using Solution‐Gated Graphene Transistors in Prostate Cancer Diagnosis

Abstract

The incidence of prostate cancer (PCa) in men globally increases as the standard of living improves. Blood serum biomarker prostate-specific antigen (PSA) detection is the gold standard assay that do not meet the requirements of early detection. Herein, a solution-gated graphene transistor (SGGT) biosensor for the ultrasensitive and rapid quantification detection of the early prostate cancer-relevant biomarker, miRNA-21 is reported. The designed single-stranded DNA (ssDNA) probes immobilized on the Au gate can hybridize effectively with the miRNA-21 molecules targets and induce the Dirac voltage shifts of SGGT transfer curves. The limit of detection (LOD) of the sensor can reach 10 −20 M without amplification and any chemical or biological labeling. The detection linear range is from 10 −20 to 10 −12 M. The sensor can realize real-time detection of the miRNA-21 molecules in less than 5 min and can well distinguish one-mismatched miRNA-21 molecule. The blood serum samples from the patients without RNA extraction and amplification are measured.The results demonstrated that the biosensor can well distinguish the cancer patients from the control group and has higher sensitivity (100%) than PSA detection (58.3%). Contrastingly, it can be found that the PSA level is not directly related to PCa.

Unamplified and Label‐Free Detection of HPV16 DNA Using CRISPR‐Cas12a‐Functionalized Solution‐Gated Graphene Transistors

“…The Fabrication of the SGGT Sensor: The preparation process of SGGT could be referred to the previous report. [23] The electrodes (Cr, 3 nm; Au, 30 nm) of SGGT were deposited on the glass substrate. The graphene was transferred to the channel between the source and drain by wet chemical method.…”

Advancements in Airborne Viral Nucleic Acid Detection with Wearable Devices

“…Deng et al [85] miRNA-21 A solution-gated graphene transistor that produces a shift in the Dirac point voltage upon hybridization with immobilized DNA probes on the Au gate electrode.…”

“…Another example of FETs adaptable for wearables is the work of Deng et al [85] in which they recently developed a solution-gated graphene transistor (SGGT) (Figure 5C) to detect miRNA-21 (a biomarker for the early diagnosis of prostate cancer) in clinical samples with minimal sample preparation. The presence of the target miRNA was determined by analyzing the position shift of the Dirac point voltage upon hybridization with immobilized DNA probes on the gate electrode.…”

Carboxyl graphene modified PEDOT:PSS organic electrochemical transistor for in situ detection of cancer cell morphology

“…Recently, organic electrochemical transistors (OECTs) have been generally used in biosensor research for the detection of several analytes, such as ions, 12,13 glucose, 14 dopamine, 15 proteins, 16 DNA, 17,18 cells, 19 bacteria, 20 alkaline phosphatase, 21 miRNA-21, 22 SARS-CoV-2, 23 and COVID-19. 24,25 Flexible OECT device arrays can be used as wearable on-skin detectors to record electrocardiogram signals for monitoring heart conditions in real-time.…”