Unauthorised antibiotic treatments in beekeeping

Benetti, C.; Dainese, Nicoletta; Biancotto, Giancarlo; Piro, Roberto

doi:10.1016/j.aca.2004.04.070

Cited by 24 publications

(5 citation statements)

References 13 publications

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“…Nevertheless, American foulbrood also presents resistance against macrolides. The best methodology for detecting macrolides in honey samples is solid-phase extraction followed by liquid chromatography tandem mass spectrophotometry [62]. Another type of antibiotic used against American and European foulbrood is streptomycin.…”

Section: Antibiotic Treatments and The Analytical Methods For Detectimentioning

confidence: 99%

American Foulbrood and the Risk in the Use of Antibiotics as a Treatment

Mejías¹

2020

Modern Beekeeping - Bases for Sustainable Production

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Honeybees (Apis mellifera) crucially pollinate agricultural crops and endemic species, in addition to producing various apiculture products. The most economically relevant and abundant beehive product is honey, a sweet substance made from the secretions of melliferous plants. Honey is a natural food rich in nutrients, including certain bioactive compounds inherited from floral nectar and pollen. Among the most dangerous diseases for bees is American foulbrood. Spores of the causative microorganism, Paenibacillus larvae, can contaminate larvae food or the operculum wax in which larval stages of honeybees are kept. Infection is further promoted by common apiculture practices, such as reusing inert material contaminated with spores, even after months of storage. American foulbrood is untreatable, and management implicates completely incinerating the infected hive and all material that could have come into contact with pathogenic spores. The purpose of such drastic measures is to decrease propagation risk for other beehives. While evidence indicates that antibiotics could effectively control and combat this disease; antibiotic use is prohibited in most honey-producing countries due to increased risks to microbial resistance. Antibiotic residues in honey can affect consumer health, since the natural biological attributes of honey can be altered.

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Section: Antibiotic Treatments and The Analytical Methods For Detectimentioning

confidence: 99%

American Foulbrood and the Risk in the Use of Antibiotics as a Treatment

Mejías¹

2020

Modern Beekeeping - Bases for Sustainable Production

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“…There are mainly ternary mobile phases consisting of mixtures of ACN-formic acid-water [10]; methanol-01% v/v TFA in ACN or 0.1% v/v TFA in water [22]; methanol-ACN-oxalic acid [24]; ACN-heptafluorobutiric acid-ethyl acetate [23]; water-potassium phosphate buffer-THF [26]. Also, some binary mobile phases have been proposed [21,27].…”

Section: Lc Conditions 321 Mobile-phase Compositionmentioning

confidence: 99%

“…Limited information is available related to the analysis of tylosin residues in honey and other sugar matrices [21,22]. Microbiological assays were firstly used, but due to their lack of specificity chromatographic techniques were preferred.…”

Section: Introductionmentioning

confidence: 99%

“…Microbiological assays were firstly used, but due to their lack of specificity chromatographic techniques were preferred. LC with UV detection at 280 nm [11] was used, although the coupling LC-MS results were more suitable, because this technique allows better identification, providing good detection limits for TA and other potentially present antibiotics [10,21,23]. With regard to the sample preparation, the most common procedures include a dilution of sample at alkaline pH and an isolation step based on the use of SPE on C18 or polymeric cartridges [10,[21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Trace analysis of antibacterial tylosin A, B, C and D in honey by liquid chromatography-electrospray ionization-mass spectrometry

et al. 2006

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A new LC-ESI-MS method was developed for the determination of residues of the antibacterial tylosins A, B, C and D in honey. The procedure employed an SPE on polymeric cartridges for the isolation of tylosins from diluted honey. Chromatographic separation of the tylosins was performed on a C18 column (150 x 4.60 mm2 ID, 5 microm) using a ternary gradient made of formic acid 1% in water (solvent A), methanol (solvent B) and ACN (solvent C) as mobile phase, at 30 degrees C and at a flow rate of 0.8 mL/min. Average analyte recoveries for the studied compounds ranged from 89 to 106% in replica sets of fortified honey samples. The detection limits for the four drugs studied were between 2 and 3 microg/kg. The developed method has been applied to the analysis of tylosin residues in honey from veterinarian treated beehives fed with the technical product, which contains the four compounds and is a new candidate antibiotic to treat American foulbrood disease of honey bee colonies.

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“…The detection limits with LC/MS/MS were all less than 0.1 μg/kg and a limit of confirmation of 0.4 μg/kg was reported for tylosin. Benetti et al 18 also recently reported the development of an LC/MS/MS method specifically for tylosin in honey with a detection capability of 4.42 μg/kg. The methods reported by Wang and Benetti et al .…”

mentioning

confidence: 99%

Determination of lincomycin and tylosin residues in honey by liquid chromatography/tandem mass spectrometry

Thompson

Noot

Calvert

et al. 2005

Rapid Comm Mass Spectrometry

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A simple and rapid analytical method was developed for the determination of lincomycin and tylosin residues in honey as part of field studies examining the efficacy and target animal safety of these antibiotics to control American foulbrood disease in honey bees. Residues of the antibiotics were determined using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Honey samples were diluted and injected directly into the LC/MS/MS system without additional cleanup by solid-phase extraction or liquid-liquid partitioning. A six-port valve system was utilized to selectively route eluant from the LC column into the mass spectrometer only during a relatively short portion of the chromatographic run corresponding to the elution of the analytes of interest. Minimal contamination of the MS source chamber was observed despite the analysis of large numbers of samples. Using internal standard quantitation, excellent accuracy and precision were obtained with no apparent matrix-to-matrix variation. Based on the analysis of fortified replicates, the mean percent deviation from the theoretical concentration and the percent relative standard deviation were both less than 10% for tylosin over an analytical range of 10-1000 microg/kg. Slightly higher mean percent deviations and relative standard deviations were observed for the analysis of lincomycin in fortified replicate samples. The method detection limits were determined to be 5 and 2 microg/kg for lincomycin and tylosin, respectively.

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Unauthorised antibiotic treatments in beekeeping

Cited by 24 publications

References 13 publications

American Foulbrood and the Risk in the Use of Antibiotics as a Treatment

American Foulbrood and the Risk in the Use of Antibiotics as a Treatment

Trace analysis of antibacterial tylosin A, B, C and D in honey by liquid chromatography-electrospray ionization-mass spectrometry

Determination of lincomycin and tylosin residues in honey by liquid chromatography/tandem mass spectrometry

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