Recent efforts have been made to monitor the seasonal metrics of plant canopy variations globally from space, using optical remote sensing. However, phenological estimations based on vegetation indices (VIs) in high-latitude regions such as the pan-Arctic remain challenging and are rarely validated. Nevertheless, pan-Arctic ecosystems are vulnerable and also crucial in the context of climate change. We reported the limitations and challenges of using MODerate-resolution Imaging Spectroradiometer (MODIS) measurements, a widely exploited set of satellite measurements, to estimate phenological transition dates in pan-Arctic regions. Four indices including normalized vegetation difference index (NDVI), enhanced vegetation index (EVI), phenology index (PI), plant phenological index (PPI) and a MODIS Land Cover Dynamics Product MCD12Q2, were evaluated and compared against eddy covariance (EC) estimates at 11 flux sites of 102 site-years during the period from 2000 to 2014. All the indices were influenced by snow cover and soil moisture during the transition dates. While relationships existed between VI-based and EC-estimated phenological transition dates, the R 2 values were generally low (0.01-0.68). Among the VIs, PPI-estimated metrics showed an inter-annual pattern that was mostly closely related to the EC-based estimations. Thus, further studies are needed to develop region-specific indices to provide more reliable estimates of phenological transition dates. sensors [5], is measurable by remote sensing methods [6][7][8]. However, the scale disparity between the spatial extent of the organisms and the spatial resolution of the sensor results in an ambiguous mixture of target and background or signal and noise [9,10].LSP metrics are often compared with direct observations of physical changes in the canopy structure. Eddy covariance (EC) measurements of carbon exchange offer a spatially and temporally perspective for extracting key phenological dates by assessing seasonal cycles of gross primary productivity (GPP) [8,11]. The long-term durability of CO 2 flux measurements in EC tower sites worldwide allows comparisons with remote sensing-based estimates at spatial footprints similar to coarse and medium resolution satellite pixels [4,12,13]. Productive efforts have been made to improve the estimates of phenological transitions based on remote sensing observations [12,[14][15][16], using new methods such as digital cameras [8,17], developing improved vegetation indices, and applying remotely sensed solar-induced fluorescence (SIF) from the Global Ozone Monitoring Experiment-2 (GOME-2) and the Orbiting Carbon Observatory-2 (OCO-2) [18][19][20].Optical remote sensing observations are widely applied to estimate key phenological dates, often using vegetation indices (VIs) based on a combination of reflectances in different spectral bands. The most commonly applied VI is the normalized difference vegetation index (NDVI), which involves red and near-infrared reflectances. The NDVI is sufficiently stable to permit meaningful ...