Uncoated iron oxide nanoparticles as a tool for human mesenchymal stromal cells labelling in vivo

Enukashvily, N. I.; Levchuk, Ksenia; Bagaeva, V V; Bogolyubov, D. S.; Bogolyubova, I. O.; Shumeev, A.N.; Zolina, T.; Kotova, A.; Maslennikova, I. I.; Artamonov, A. V.; Marchenko, N. V.; Mindukshev, Igor; Kotkas, I. E.

doi:10.1016/j.jcyt.2018.02.089

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“…The powder samples were dried at 100°C under vacuum for 24 h prior to fabrication of the KBr pellet. Spectra were recorded with a resolution of 2 cm - 1 .…”

Section: Methodsmentioning

confidence: 99%

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In vitro inhibition of tumor growth by low-dose iron oxide nanoparticles activating macrophages

et al. 2019

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Macrophages as immunocyte are attracting more and more attention in cancer therapy. Our previous study observed that dimercaptosuccinic acid (DMSA)-coated Fe3O4 magnetic nanoparticles triggered comprehensive immune responses of mouse macrophages (RAW264.7 cells) and induced production of many kinds of cytokines. This study investigated the effects of Fe3O4 magnetic nanoparticles on RAW264.7 cells proliferation, migration, and inhibition of tumor growth in vitro. Fe3O4 magnetic nanoparticles had an average size of about 11 nm with good dispersibility and uniformity. Fe3O4 magnetic nanoparticles internalized efficiently into RAW264.7 cells. Through Cell Counting Kit-8 (CCK-8) detection, the proliferation of RAW264.7 cells significantly increased by the low-dose Fe3O4 magnetic nanoparticles (50 µg/mL) treatment. The results of wound-healing and Transwell assays both displayed a significant promotion of the RAW264.7 cells migratory capability compared with control group ( P<0.01). It is interesting to find that a large number of proliferated RAW264.7 cells were activated to surround quickly and attack mouse liver cancer cell (Hepa1-6) cells by Fe3O4 magnetic nanoparticles. The growth of Hepa1-6 cells was effectively inhibited according to microscope imaging and flow cytometry analysis. The inhibition may be cooperative effects of RAW264.7 cells proliferation, migration, and immune activation. The results suggest potential clinical value of low-dose iron oxide nanomaterials in cancer therapy.

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“…The powder samples were dried at 100°C under vacuum for 24 h prior to fabrication of the KBr pellet. Spectra were recorded with a resolution of 2 cm - 1 .…”

Section: Methodsmentioning

confidence: 99%

“…Iron oxide nanoparticles are widely applied in cell labeling, 1,2 drug delivery with tissue specificity, 3–5 and magnetic resonance imaging (MRI) contrast enhancement. 6–8 Many studies focus on fabrication methods to prepared functionalized nanomaterials based on iron oxide nanoparticles for their better application.…”

Section: Introductionmentioning

confidence: 99%