Unconditioned commercial embryo culture media contain a large variety of non-declared proteins: a comprehensive proteomics analysis

Dyrlund, Thomas F.; Kirkegaard, Kirstine; Poulsen, Ebbe Toftgaard; Sanggaard, Kristian W.; Hindkjær, Johnny; Kjems, Jørgen; Enghild, Jan J.; Ingerslev, Hans Jakob

doi:10.1093/humrep/deu220

Cited by 65 publications

(45 citation statements)

References 43 publications

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“…Nonetheless, the use of serum albumin in place of serum has not contributed much to the development of chemically defined media. First, most, if not all, commercial serum albumin versions contain >100 serum proteins, although these admixtures have very low concentrations 142, 143. Second, albumin can bind to potential toxins, like phthalates144 (common plasticizers) or endotoxins.…”

Section: Current Issues With Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

396

278

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BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

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Section: Current Issues With Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

396

278

View full text Add to dashboard Cite

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“…Tha proteomic analysis of human purified serum albumin that is added in all culture media suggested that a number of proteins, mainly involved in immune response and inflammation, are concomitantly introduced to the media during addition. This observation complicates the interpretation of the results from proteomic studies on spent culture media, but also raises questions regarding the impact of the identified proteins on embryo development (107).…”

Section: Proteomics and Embryo Qualitymentioning

confidence: 99%

The Use of Proteomics in Assisted Reproduction

Kosteria

Anagnostopoulos

Kanaka‐Gantenbein

et al. 2017

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“…In Dyrlund et al's recent study [13], 110 proteins other than albumin were identiied in commercially available unconditioned culture media supplemented with puriied human serum albumin products. Probably it is not an issue in clinical practice since these products have been proven themselves for decades.…”

Section: Analysis Of the Embryo Culture Mediummentioning

confidence: 99%

Non‐Invasive Assessment of the Embryo Viability via the Analysis of the Culture Media

Montskó¹,

Zrínyi²,

Várnagy³

et al. 2017

Embryo Cleavage

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Infertility in recent years is a growing public health issue throughout the developed world. Assisted reproductive techniques, especially in vitro fertilization, have the potential to partially overcome the low natural reproductive ratio. Nowadays, single embryo transfer gains grounds in clinical practice, urging the development of more reliable methods for selecting the best embryo. In the traditional clinical practice, embryos are selected for transfer based on morphological evaluation. In vitro culturing of embryos also provides a very important material for further non-invasive evaluation by means of examining a biomarker in the spent culture medium (SEC). Current measure methods concentrate on the metabolomic activity of the developing embryos none compounds. These studies are mainly utilizing the tools of modern analytics and proteomics. In a paper published by Montskó et al. in 2015, the alpha-1 chain of the human haptoglobin molecule was described as a quantitative biomarker of embryo viability. In a series of retrospective, blind experiments achieved more than 50% success rate. This chapter summarizes the currently available metabolomic and proteomic approaches as the non-invasive molecular assessment of embryo viability.

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Unconditioned commercial embryo culture media contain a large variety of non-declared proteins: a comprehensive proteomics analysis

Abstract: The study was supported by the Danish Agency for Science, Technology and Innovation. Research at the Fertility Clinic, Aarhus University Hospital is supported by an unrestricted grant from Merck Sharp & Dohme Corp and Ferring. The authors declare no conflicts of interest.

Cited by 65 publications

References 43 publications

Animal‐cell culture media: History, characteristics, and current issues

Animal‐cell culture media: History, characteristics, and current issues

The Use of Proteomics in Assisted Reproduction

Non‐Invasive Assessment of the Embryo Viability via the Analysis of the Culture Media

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