Uncoupling protein 1 affects the yeast mitoproteome and oxygen free radical production

Douette, Pierre; Gerkens, Pascal; Navet, Rachel; Leprince, Pierre; Pauw, Edwin De; Sluse, Francis

doi:10.1016/j.freeradbiomed.2005.08.024

Cited by 24 publications

(41 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A concentration of ∼ 1 μg of UCP1 per mg of mitochondrial protein was detected, confirming previous results using similar methods [21]. A concentration of ∼ 1 μg of UCP1 per mg of mitochondrial protein was detected, confirming previous results using similar methods [21].…”

Section: Expression Of Ucp1 In Saccharomyces Cerevisiaesupporting

confidence: 89%

“…cerevisiae (INVSc1 strain) cells were transformed with either a rat UCP1 low-expressing construct or a plasmid containing an empty vector (pYES2.1) (Invitrogen) [21]. cerevisiae (INVSc1 strain) cells were transformed with either a rat UCP1 low-expressing construct or a plasmid containing an empty vector (pYES2.1) (Invitrogen) [21].…”

Section: Expression Of Ucp1 In Saccharomyces Cerevisiaementioning

confidence: 99%

See 1 more Smart Citation

Uncoupling protein 1 inhibition by purine nucleotides is under the control of the endogenous ubiquinone redox state

Świda-Barteczka

Woyda-Płoszczyca

Sluse

et al. 2009

Biochemical Journal

View full text Add to dashboard Cite

We studied non-esterified fatty acid-induced uncoupling of heterologously expressed rat UCP1 (uncoupling protein 1) in yeast mitochondria, as well as UCP1 in rat BAT (brown adipose tissue) mitochondria. The proton-conductance curves and the relationship between the ubiquinone reduction level and membrane potential were determined in non-phosphorylating BAT and yeast mitochondria. The ADP/O method was applied to determine the ADP phosphorylation rate and the relationship between the ubiquinone reduction level and respiration rate in yeast mitochondria. Our studies of the membranous ubiquinone reduction level in mitochondria demonstrate that activation of UCP1 leads to a purine nucleotide-sensitive decrease in the ubiquinone redox state. Results obtained for non-phosphorylating and phosphorylating mitochondria, as the endogenous ubiquinone redox state was gradually varied by a lowering rate of the ubiquinone-reducing or ubiquinol-oxidizing pathways, indicate that the endogenous ubiquinone redox state has no effect on non-esterified fatty acid-induced UCP1 activity in the absence of GTP, and can only regulate this activity through sensitivity to inhibition by the purine nucleotide. At a given oleic acid concentration, inhibition by GTP diminishes when ubiquinone is reduced sufficiently. The ubiquinone redox state-dependent alleviation of UCP1 inhibition by the purine nucleotide was observed at a high ubiquinone reduction level, when it exceeded 85-88%.

show abstract

Section: Expression Of Ucp1 In Saccharomyces Cerevisiaesupporting

confidence: 89%

Section: Expression Of Ucp1 In Saccharomyces Cerevisiaementioning

confidence: 99%

Uncoupling protein 1 inhibition by purine nucleotides is under the control of the endogenous ubiquinone redox state

Świda-Barteczka

Woyda-Płoszczyca

Sluse

et al. 2009

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…Here we tested the uncoupling activity of HDMCP in a yeast expression system for two reasons: Firstly, so far no uncoupling proteins have been found on yeast mitochondrial inner membrane and none of the 35 mitochondrial carrier proteins have uncoupling activity [28], which could reduce the potential bias. Secondly, yeast was proved to be a good expression system to evaluate the uncoupling activities of UCP1-3 [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

HDMCP uncouples yeast mitochondrial respiration and alleviates steatosis in L02 and hepG2 cells by decreasing ATP and H2O2 levels: A novel mechanism for NAFLD

Jin¹,

Yang²,

Chen³

et al. 2009

Journal of Hepatology

View full text Add to dashboard Cite

“…[26][27][28] When NNt was induced (lactate and galactose), NNt-expressing cells exhibited slower log phase growth rates than Wt cells (Fig. 2A).…”

Section: Growth Rates In Yeast Clonesmentioning

confidence: 98%

A High-Throughput Assay for Modulators of NNT Activity in Permeabilized Yeast Cells

et al. 2011

View full text Add to dashboard Cite

Uncoupling protein 1 affects the yeast mitoproteome and oxygen free radical production

Cited by 24 publications

References 46 publications

Uncoupling protein 1 inhibition by purine nucleotides is under the control of the endogenous ubiquinone redox state

Uncoupling protein 1 inhibition by purine nucleotides is under the control of the endogenous ubiquinone redox state

HDMCP uncouples yeast mitochondrial respiration and alleviates steatosis in L02 and hepG2 cells by decreasing ATP and H2O2 levels: A novel mechanism for NAFLD

A High-Throughput Assay for Modulators of NNT Activity in Permeabilized Yeast Cells

Contact Info

Product

Resources

About