Uncovering the diversity and contents of gene cassettes in class 1 integrons from the endophytes of raw vegetables

Zhao, Chen; Su, Xiaoxuan; Xu, Meirong; An, Xin-Li; Su, Jian‐Qiang

doi:10.1016/j.ecoenv.2022.114282

Cited by 8 publications

(2 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antimicrobial resistance is still a major public concern, topping the World Health Organization's (WHO) list of health concerns [59,60]. Recent studies have demonstrated that antimicrobial resistance genes (ARGs) are frequently acquired via horizontal gene transfer (HGT) using mobile genetic elements, resulting in the establishment and evolution of antimicrobial resistance bacteria [61,62].…”

Section: Antibiotic Resistance Genesmentioning

confidence: 99%

Genome Sequence and Characterisation of Peribacillus sp. Strain AS_2, a Bacterial Endophyte Isolated from Alectra sessiliflora

Maela,

Serepa-Dlamini

2023

Microbiology Research

View full text Add to dashboard Cite

Peribacillus sp. AS_2, a leaf endophytic bacterium isolated from the medicinal plant Alectra sessiliflora, was previously identified using the 16S rRNA gene sequence. The draft genome of AS_2 had a 5,482,853 bp draft circular chromosome, 43 contigs, N50 of 360,633 bp and an average G + C% content of 40.5%. Whole genome sequencing and phenotypic analysis showed that AS_2 was Gram-positive, endospore-forming, motile, and rod-shaped and it showed a high sequence similarity with P. frigoritolerans DSM 8801T. Genomic digital DNA–DNA hybridisation (dDDH) between strain AS_2 and Peribacillus frigoritolerans DSM 8801T and P. castrilensis N3T was 84.8% and 79.2%, respectively, and the average nucleotide identity (ANI) of strain AS_2 with P. frigoritolerans DSM 8801T and P. castrilensis N3T was 97.0% and 96.7%, respectively. The antiSMASH software predicted a total of eight secondary metabolite gene clusters comprising non-ribosomal peptide synthetase (NRPS) type koranimine, terpenes, and siderophore clusters. Strain AS_2 also displayed genes involved in endophytic lifestyle and antibiotic resistance gene clusters such as small multidrug resistance antibiotic efflux pumps (qacJs). Using the multilocus sequence analysis (MLSA), together with the phenotypic data and genomic analysis, we demonstrated that strain AS_2 is a subspecies of P. frigoritolerans DSM 8801T. Genome sequencing of Peribacillus sp. AS_2 from medicinal plants provides valuable genomic information and allows us to further explore its biotechnological applications.

show abstract

Section: Antibiotic Resistance Genesmentioning

confidence: 99%

Genome Sequence and Characterisation of Peribacillus sp. Strain AS_2, a Bacterial Endophyte Isolated from Alectra sessiliflora

Maela,

Serepa-Dlamini

2023

Microbiology Research

View full text Add to dashboard Cite

show abstract

“…More importantly, many scholars have reported that the application of manure not only leads to the enrichment of ARGs in soil, but also signi cantly increases the enrichment of ARGs in plants (SHIHAI L et al,2024).Planting vegetables in manure-amended soils may lead to an increase in the abundance and diversity of some ARGs in vegetables ready for retail on the market after harvest (XIAOTING F et al,2023; CAIXIA Z et al,2022).A variety of tetracycline resistance genes and MGEs with high relative abundance were detected in the endophytic bacteria of three kinds of vegetable leaves harvested in the vegetable eld with manure application. MGEs have a certain risk of promoting the migration and diffusion of ARGs in vegetable endophytic bacteria, which means that eating these vegetables raw may pose a threat to human health (Wang.,2021).Yin et aldetected 205 ARGs from the phyllosphere of six vegetables, among which multidrug resistance genes were the most abundant (YUE Y et al,2022).Chen et alshowed that long-term application of chicken manure as an organic fertilizer to irrigate farmland soil would signi cantly increase the abundance of ARGs in the phyllosphere, with the highest enrichment factor of 2638 times(CHEN Q-L et al.,2018).Therefore, raw vegetables may be a potential way for soil ARGs to enter the human body, and its migration and driving mechanism to the lower end of the food chain through horizontal transfer need to be further explored.…”

Section: Introductionmentioning

confidence: 99%

Fecal antibiotic resistance genes were transferred through the distribution of soil-lettuce-snail food chain

Zhang

Zhao

Chen

et al. 2023

Environ Sci Pollut Res

View full text Add to dashboard Cite

Massive antibiotic resistance genes (ARG) were detected in the soil modi ed by manure, which may affect human life safety through the food chain. However, the transmission of ARGs through the soil-plant-animal food chain is still unclear. Therefore, this study used high-throughput quantitative PCR technology to explore the effects of pig manure application on ARGs and bacterial communities in soil, lettuce phyllosphere and snail excrement.The results showed that a total of 384 ARGs and 48 MEGs were detected in all samples after 75 days of incubation. The diversity of ARGs and MGEs in soil components increased signi cantly by 87.04%and 40%with the addition of pig manure. The absolute abundance of ARGs in the phyllosphere of lettuce was signi cantly higher than that of the control group, with a growth rate of 212.5%.Six common ARGs were detected between the three components of the fertilization group, indicating that there was internal transmission of fecal ARGs between the trophic levels of the food chain.Firmicutes and Proteobacteria were identi ed as the dominant host bacteria in the food chain system, which were more likely to be used as carriers of ARGs to promote the spread of resistance in the food chain.The results were used to evaluate the potential ecological risks of livestock and poultry manure and provide theoretical basis and scienti c support for the formulation of ARGs prevention and control policies.

show abstract

Impact of biochar on the antibiotic resistome and associated microbial functions in rhizosphere and bulk soil in water-saving and flooding irrigated paddy fields

Zhang,

Xu,

Wang

et al. 2024

Environmental Pollution

View full text Add to dashboard Cite

Uncovering the diversity and contents of gene cassettes in class 1 integrons from the endophytes of raw vegetables

Cited by 8 publications

References 66 publications

Genome Sequence and Characterisation of Peribacillus sp. Strain AS_2, a Bacterial Endophyte Isolated from Alectra sessiliflora

Genome Sequence and Characterisation of Peribacillus sp. Strain AS_2, a Bacterial Endophyte Isolated from Alectra sessiliflora

Fecal antibiotic resistance genes were transferred through the distribution of soil-lettuce-snail food chain

Impact of biochar on the antibiotic resistome and associated microbial functions in rhizosphere and bulk soil in water-saving and flooding irrigated paddy fields

Contact Info

Product

Resources

About