Objective: Burnout among emergency medical technicians is a serious problem affecting delivery of quality emergency medical services. Although the repetitive nature of the job and lower education level requirements for technicians have been reported as risk factors, little is known about the influence of burden of responsibility, degree of supervisor support, and home environment on burnout among emergency medical technicians. This study aimed to test the hypothesis that burden of responsibility, degree of supervisor support, and home environment increase burnout probability. Methods: A web-based survey was conducted among emergency medical technicians in Hokkaido, Japan from July 26, 2021 to September 13, 2021. A total of 21 facilities were randomly selected from 42 fire stations. Prevalence of burnout was measured using the Maslach Burnout-Human Services Survey Inventory.Burden of responsibility was measured using a visual analog scale. Occupational background was also measured. Supervisor support was measured using the Brief Job Stress Questionnaire. Family-work negative spillover was measured using the Survey Work-Home Interaction-NijmeGen-Japanese. The cutoff value for burnout syndrome was defined as emotional exhaustion ≥ 27 and/or depersonalization ≥ 10. Results: A total of 700 survey respondents were included, and 27 surveys with missing data were excluded. The suspected burnout frequency was 25.6%. Covariates were adjusted using multilevel logistic regression model analysis, and low supervisor support (OR,1.421; 95% CI:1.136-1.406, p < .001) and high family-work negative spillover (OR:1.264, 95% CI:1.285-1.571, p < .001) were independent factors that predicted higher probability of burnout. Conclusion: This study indicated that focusing on improvement of supervisor support for emergency medical technicians and creating supportive home environments may assist in reducing burnout frequency.