Understanding bacterial colonisation of built cultural heritage

Skipper, Philip J. A.

doi:10.14575/gl/rehab2014/101

Cited by 1 publication

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“…Despite bacteria not being an object of this study, the frequent isolation of a filamentous Actinobacteria was an interesting result. Some organisms from this phylum (e.g., Streptomyces) have been associated with damaged limestone, frequently promoting a decrease in pH, therefore working as biodegradation phenomena indicators [24,126].…”

Section: Metabarcoding Vs Culture-dependent Diversity and Biodeterior...mentioning

confidence: 99%

Uncovering the Fungal Diversity Colonizing Limestone Walls of a Forgotten Monument in the Central Region of Portugal by High-Throughput Sequencing and Culture-Based Methods

et al. 2022

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Fungal organisms are considered one of the most relevant stone colonizers, and biodeteriogens. They are ubiquitous heterotrophs, metabolically versatile, ranging from generalist to extremophiles. Limestone, a sedimentary rock characterized by high levels of calcium carbonate, has low compressive strength and hardness and high porosity. These features make it highly susceptible to fungal colonization and an exceptional target for biodeterioration. Understanding the mycobiome composition associated with different biodeterioration scenarios is key for the development of effective guidelines and strategies for preventive conservation and viable maintenance of our cultural heritage. In this work, a thorough analysis of the fungal community composition on the Lemos Pantheon, a limestone-built artwork located in Portugal, was performed using high-throughput sequencing complemented with culture-based methods. The combined results allowed a detailed characterization of the fungal communities of each analyzed spot, revealing highly diverse and dissimilar communities according to the type of biodeterioration observed. In addition, we verified that both cultivation and metagenomics methodologies should be employed synergistically to tackle inherent limitations.

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Section: Metabarcoding Vs Culture-dependent Diversity and Biodeterior...mentioning

confidence: 99%