Understanding Genetic Diversity of the Liver Fluke Fasciola hepatica

Dosay-Akbulut, Mine; Trudgett, Alan; Stanhope, Michael J.

doi:10.1515/znc-2005-9-1018

Cited by 10 publications

(9 citation statements)

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“…This is comparable to the 1 to 7% reported elsewhere (Dosay-Akbulut et al 2005) for both the ribosomal ITS 1 and mitochondrial ND1 genes of the fluke. These values are approximately twice greater than those reported by Blouin et al (1999) working with the parasitic nematode Heterorhabitis marelatus, using values derived from an analysis of a 600-bp fragment contained within the approximately 7,000-bp sequence used in this study.…”

Section: Discussionsupporting

confidence: 89%

“…The recent development of microsatellite markers (Hurtrez-Bousses et al 2004) and their application to Bolivian samples of F. hepatica from cattle, sheep, and pig hosts have similarly indicated the existence of genetic polymorphism between flukes from distinct definitive hosts, but again, little detail of the source of these flukes was given. Dosay-Akbulut et al (2005) used the DNA sequencing of part of the mitochondrial ND1 gene to show that, although flukes from sheep and cattle were not distinct, evolutionary lineage differences between individual flukes may be detected using mitochondrial DNA. However, this study was again limited in the number of samples analysed and lacked detail with regards to their provenance.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for multiple mitochondrial lineages of Fasciola hepatica (liver fluke) within infrapopulations from cattle and sheep

Walker¹,

Prodöhl²,

Fletcher³

et al. 2007

Parasitol Res

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The economic, veterinary, and medical impact of the parasite Fasciola hepatica, liver fluke, is difficult to alleviate due to increasing incidences of resistance to the principal anthelmintic drugs. These have occurred in widely separated regions. The rate of response to selection imposed by such drugs will be dependent on the genetic variation present in the F. hepatica gene pool, but this is at present unknown. We have assessed the genetic diversity of mitochondrial haplotypes found in the infrapopulation of flukes recovered from a calf of known provenance and from six other cattle and sheep hosts located in Ireland and four from elsewhere. Our results revealed that at least ten different mitochondrial composite PCR-restriction fragment length polymorphism haplotypes had been acquired by a single animal in 1 year, and there was comparable diversity in six other definitive hosts carrying field-acquired infections. The extent of divergence between these fluke lineages suggests that they predate the last ice age and, thus, cannot have developed in Northern Europe. A consequence of this high level of diversity is that there will be frequent selection for anthelmintic resistance and rapid responses to climatic changes.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Evidence for multiple mitochondrial lineages of Fasciola hepatica (liver fluke) within infrapopulations from cattle and sheep

Walker¹,

Prodöhl²,

Fletcher³

et al. 2007

Parasitol Res

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“…Previous studies have characterized Fasciola from different countries using genetic approaches (Itagaki and Tsutsumi 1998;Agatsuma et al 2000;Huang et al 2004;Alasaad et al 2007;Lin et al 2007;Vara-Del Río et al 2007;Alasaad et al 2008;Ali et al 2008;Le et al 2008;Lotfy et al 2008); however, little is known about genetic identification of F. hepatica from Turkey (Dosay-Akbulut et al 2005;Kuk et al 2005Kuk et al , 2006Semyenova et al 2006). Therefore, the objective of the present study was to describe the molecular identification of the F. hepatica samples from different geographical locations in Turkey (Elazig, Malatya, Samsun) by sequences of the ITS-2 rDNA.…”

Section: Introductionmentioning

confidence: 95%

Genetic identification of Fasciola hepatica by ITS-2 sequence of nuclear ribosomal DNA in Turkey

2009

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The trematodes of the genus Fasciola are the common liver flukes of a range species of animals and have a global geographical distribution. They can generally be distinguished on the basic of their morphology. ITS-2 ribosomal DNA sequences have been used to characterize the liver flukes as a specific marker from different geographical regions which include F. hepatica, F. gigantica, and an intermediate Fasciola. To determine the phylogenetic location of F. hepatica of Turkey origin based on ITS-2 rDNA molecular data, adult F. hepatica trematodes were collected from the liver naturally infected sheep from different geographical locations in Turkey (Elazig, Malatya, Samsun). ITS-2 rDNA were cloned, sequenced, and compared with published sequences ITS-2 rDNA of other species of trematodes in the family Fasciolidae using the GenBank Blast program. The only one ITS-2 sequence had defined for the examined Turkish F. hepatica samples. The phylogenetic trees constructed based upon the ITS-2 sequences from Turkey by multiple tree-building methods in MEGA revealed a close relationship with isolates of F. hepatica, F. gigantica, and Fasciola sp. The present study is the first demonstration of the existence of F. hepatica in sheep in Turkey by the genetic approach using ITS-2 rDNA as genetic marker.

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“…The other also came from eastern Europe and central Asia. A more limited study, developed in Turkey, showed the diversity of F. hepatica strains by sequencing the mitochondrial gene nad1 and the ribosomal part ITS1 (Dosay-Akbulut et al, 2005). Other previous studies (Marcilla et al, 2002;Ramadan & Saber, 2004) were developed to distinguish F. hepatica from F. gigantica, particularly in countries where both species are sympatric.…”

Section: Diversity Of Parasites In the Paragonimus Genusmentioning

confidence: 99%

Biodiversity of flukes

Dreyfuss

Rondelaud

2008

Parasite

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Summary :As many others parasites, speciation of flukes depends on the genetic characteristics and on ploidia. Ploidia of flukes can be different in a same species. In Asia, diploid, triploid and hybrid (2n/3n) populations are encountered. The comparison of morphological parameters between diploid and triploid flukes showed that they were morphologically different. Nevertheless, a genetic relationship between parthenogenetic organisms would exist regardless of their ploidia. In the Fasciola genus, the main consequence of the high level of diversity is the frequent probability of development of resistance to anthelmintics and fast adaptation to climatic changes. In the Paragonimus genus, diversity can enhance different forms of pathogenicity, can also be related to the species of intermediate hosts, and to the definitive host. The strain of flukes plays a part in the visceral localization of P. westermani adults. DIFFICULTY OF SPECIATION IN MEDICAL PARASITOLOGYPLOIDIA P arasitology is a biological field in which taxonomy often still uses morphological and Linnean criteria. The limits of the Linnean descriptive methods were reached many decades ago: the dilemma Entamoeba histolytica/E. dispar is a demonstrative example. Similarly, the infra-specific relationships between biogeography of parasites and their biological characteristics (rhythm, pathology, reservoirs) were used to organize the taxonomy of genetically different parasitic populations (Wuchereria bancrofti, for instance). Genetic diversity cannot yet be evaluated by easy and low cost methods. The results of the biological diagnosis of parasitic diseases tend to be less precise, and decrease at the genus level (E. histolytica/E. dispar), or even the family (Opisthorchiidae, Ancylostomatidae, Paragonimidae). Protozoa are parasites for which genetic diversity can be evaluated quite easily. Helminths, particularly Digenea, cannot be analyzed easily, because many species have a variable ploidia, which modifies their morphology and DNA size. A comparative morphometric study, by light microscopy, of eggs and adults from three populations with different sizes of Fasciola gigantica, and the morphology of adults, under scanning electron microscopy, showed variations in the sizes of eggs and adults, whereas the surface was similar using electronic morphology (Srimuzipo et al., 2000). The observations on mitotic metaphase chromosomes revealed a diploid type (2n = 20) in all three size-races. The speciation of Digenea can no longer be evaluated by simple morpholo-

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Understanding Genetic Diversity of the Liver Fluke Fasciola hepatica

Cited by 10 publications

References 0 publications

Evidence for multiple mitochondrial lineages of Fasciola hepatica (liver fluke) within infrapopulations from cattle and sheep

Evidence for multiple mitochondrial lineages of Fasciola hepatica (liver fluke) within infrapopulations from cattle and sheep

Genetic identification of Fasciola hepatica by ITS-2 sequence of nuclear ribosomal DNA in Turkey

Biodiversity of flukes

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