Understanding mild cell disintegration of microalgae in bead mills for the release of biomolecules

García, Edgar Suárez; Lo, Calvin; Eppink, M.H.M.; Wijffels, René H.; Berg, C. van den

doi:10.1016/j.ces.2019.04.008

Cited by 30 publications

(8 citation statements)

References 48 publications

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“…Contrary to what is usual in diatoms, the P. tricornutum cell wall is mainly composed of sulphated glucuronomannan and is poor in silica, especially in its triradiate and fusiform morphotypes, which makes them softer [ 23 , 26 ]. On the other hand, the T. chuii cell wall is rich in a pectin-like polysaccharide, possibly conferring a relatively higher resistance to the cells [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

Improved Extraction Efficiency of Antioxidant Bioactive Compounds from Tetraselmis chuii and Phaedoactylum tricornutum Using Pulsed Electric Fields

et al. 2020

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Pulsed electric fields (PEF) is a promising technology that allows the selective extraction of high-added value compounds by electroporation. Thus, PEF provides numerous opportunities for the energy efficient isolation of valuable microalgal bioactive substances (i.e., pigments and polyphenols). The efficiency of PEF-assisted extraction combined with aqueous or dimethyl sulfoxide (DMSO) solvents in recovering pigments and polyphenols from microalgae Tetraselmis chuii (T. chuii) and Phaeodactylum tricornutum (P. tricornutum) was evaluated. Two PEF treatments were applied: (1 kV/cm/400 pulses, 3 kV/cm/45 pulses), with a specific energy input of 100 kJ/kg. The total antioxidant capacity (TAC) was positively influenced by the use of DMSO. The highest TAC in the T. chuii culture was achieved at a lower extraction time and electric field than for P. tricornutum. The use of DMSO only improved the polyphenols′ extraction for P. tricornutum, whereas the PEF and extraction time were more important for T. chuii. Carotenoids and chlorophyll a were more efficiently extracted using DMSO, while chlorophyll b levels were higher following aqueous extraction for both microalgae. In P. tricornutum, the TAC and pigment extraction efficiency were in general higher at lower extraction times. It can be concluded that PEF may be a promising alternative for the enhancement of the selective extraction of antioxidant bioactive compounds from microalgae.

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Section: Resultsmentioning

confidence: 99%

Improved Extraction Efficiency of Antioxidant Bioactive Compounds from Tetraselmis chuii and Phaedoactylum tricornutum Using Pulsed Electric Fields

et al. 2020

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“…During this procedure, a given amount of energy is applied to the cell wall, causing the release of intracellular products. The results of pretreatment depend on bead size and type, as well as agitation speed, bead filling, chamber size and geometry, biomass concentration, and suspension flow rate [ 141 , 142 ].…”

Section: Pretreatment Methodsmentioning

confidence: 99%

Recent Advances in Supercritical CO2 Extraction of Pigments, Lipids and Bioactive Compounds from Microalgae

et al. 2023

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Supercritical CO2 extraction is a green method that combines economic and environmental benefits. Microalgae, on the other hand, is a biomass in abundance, capable of providing a vast variety of valuable compounds, finding applications in the food industry, cosmetics, pharmaceuticals and biofuels. An extensive study on the existing literature concerning supercritical fluid extraction (SFE) of microalgae has been carried out focusing on carotenoids, chlorophylls, lipids and fatty acids recovery, as well as the bioactivity of the extracts. Moreover, kinetic models used to describe SFE process and experimental design are included. Finally, biomass pretreatment processes applied prior to SFE are mentioned, and other extraction methods used as benchmarks are also presented.

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“…4), destroying the cell wall [98]. Suarez Garcia et al [99] indicated that approximately 95% of the total energy released from collisions in the mill occurs in the vicinity of the agitators, where the beads are moving at the maximum speed. By contrast, the beads are almost static close to the shaft or chamber wall, where they have lower potential to collide with adjacent cells.…”

Section: Mechanisms Of Cell Disruption By Bead Millingmentioning

confidence: 99%

“…However, an optimal agitation speed is necessary from a practical point of view, since the total energy demand could become significant at higher agitation speeds compared to lower speeds. Suarez Garcia et al [99] assessed the power consumption of a bead mill under several scenarios including running the mill empty, or with beads (0.5 mm, 65% v/v loading rate) and/or microalgae cells (concentration ranging 30-155 g/L). The power consumption over a period of 20 min showed an exponential increase, from < 50 W in all scenarios to 100-350 W as the agitation speed increased from 1500 to 5500 rpm, respectively.…”

Section: Factors Influencing the Efficiency Of Enzyme Extraction By Mmentioning

confidence: 99%

“…Similarly, the heat dissipation (mainly due to friction) also increased from 17.8 to 60.8% of the total power consumed. This was probably because the movement of the beads is more chaotic at higher agitation speeds, which forces the agitator to demand more power to keep a constant stirring speed in the chamber [99].…”

Section: Factors Influencing the Efficiency Of Enzyme Extraction By Mmentioning

confidence: 99%

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Enzyme Recovery from Biological Wastewater Treatment

Liu¹,

Smith²

2020

Waste Biomass Valor

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Enzymes are high value industrial bio-catalysts with extensive applications in a wide range of manufacturing and processing sectors, including the agricultural, food and household care industries. The catalytic efficiency of enzymes can be several orders higher compared to inorganic chemical catalysts under mild conditions. However, the nutrient medium necessary for biomass culture represents a significant cost to industrial enzyme production. Activated sludge (AS) is a waste product of biological wastewater treatment and consists of microbial biomass that degrades organic matter by producing substantial quantities of hydrolytic enzymes. Therefore, enzyme recovery from AS offers an alternative, potentially viable approach to industrial enzyme production. Enzyme extraction from disrupted AS flocs is technically feasible and has been demonstrated at experimental-scale. A critical review of disruption techniques identified sonication as potentially the most effective and suitable method for enzyme extraction, which can be scaled up and is a familiar technology to the water industry. The yields of different enzymes are influenced by wastewater treatment conditions, and particularly the composition, and can also be controlled by feeding sludge with specific target substrates. Nevertheless, hydrolytic enzymes can be effectively extracted directly from waste AS without specific modifications to standard wastewater treatment processes. Purification, concentration and stabilisation/immobilisation techniques can also greatly expand the industrial application and increase the economic value and marketability of enzyme products extracted from AS. Concentrated and purified AS enzymes could readily substitute inorganic and/or commercial bioenzyme catalysts in many industrial applications including, for example, leather processing, and in detergent and animal feed formulation. Enzyme extraction from AS therefore offers significant economic benefits to the Water Industry by recovering valuable resources from wastewater. They can also optimise important waste treatment processes, such as the anaerobic digestion (AD) of sewage sludge, increasing biogas and renewable energy production. The enzyme-extracted sludge exhibits improved treatment properties, such as increased settleability, dewaterability, and anaerobic digestibility for biogas production, assisting sludge management by wastewater treatment plants (WWTPs) and enabling the further utilisation of the residual sludge. Graphic Abstract

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Understanding mild cell disintegration of microalgae in bead mills for the release of biomolecules

Cited by 30 publications

References 48 publications

Improved Extraction Efficiency of Antioxidant Bioactive Compounds from Tetraselmis chuii and Phaedoactylum tricornutum Using Pulsed Electric Fields

Improved Extraction Efficiency of Antioxidant Bioactive Compounds from Tetraselmis chuii and Phaedoactylum tricornutum Using Pulsed Electric Fields

Recent Advances in Supercritical CO2 Extraction of Pigments, Lipids and Bioactive Compounds from Microalgae

Enzyme Recovery from Biological Wastewater Treatment

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