Understanding resistance acquisition by Pseudomonas aeruginosa and possible pharmacological approaches in palliating its pathogenesis

Thakur, Mony; Khushboo, .; Kumar, Yogesh; Yadav, Vinod Kumar; Pramanik, Avijit; Dubey, Kashyap Kumar

doi:10.1016/j.bcp.2023.115689

Cited by 6 publications

(2 citation statements)

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“…The overuse of antibiotics and their imprudent disposal into the environment have led to the emergence of antibiotic resistance in organisms. 1 The antimicrobial resistance (AMR) acquired by pathogenic microbes is projected to reach 10 million by 2050. 2 Based on the urgency of new antibiotics, the WHO has listed P. aeruginosa, E. coli, and S. aureus as critical antibiotic-resistant priority pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity against Antibiotic-resistant Pathogens and Antioxidant Activity and LCMS/MS Phytochemical Content Analysis of Selected Medicinal Plants

Thakur,

Khushboo,

Yadav

et al. 2024

J. Pure Appl. Microbiol.

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Medicinal plants are a major source of numerous therapeutic agents, and the emergence of pathogenic bacteria has rekindled interest in traditional medicine systems as an alternative approach to overcoming resistance. The dried plant material of four medicinal plants, namely Terminalia arjuna (bark), Terminalia bellirica (fruit), Aegle marmelos (leaves), and Bacopa monnieri (leaves), was powdered, and aqueous extracts were prepared. The antimicrobial activity of the extracts was evaluated against three clinically important strains: Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli 385. As E. coli 385 was resistant to the broadest spectrum of antibiotics tested, it was classified as (MDR multi-drug resistant). E. coli, Bacillus subtilis, Mycobacterium smegmatis (MTTC), and Vibrio alginolyticus (ATCC) were also assessed using the agar well diffusion method for zones of inhibition and minimum inhibitory/bactericidal concentration (MIC/MBC). Clinically important strains were found to be sensitive to the aqueous extract of T. bellerica (19.51 ± 0.61 mm) with MICs ranging from 0.31 to 0.62 mg/ml. The MDR strain was also sensitive to Bellirica monnieri (16.10 ± 0.31 mm) aqueous extract. To determine the potential for a wide range of applications, the antioxidant activities of the extracts were evaluated using DPPH, ABTS, and FRAP assays. The T. arjuna plant extract exhibited the highest radical scavenging activity with the lowest EC50 values for DPPH (1.15 ± 0.061 mg/ml) and ABTS (1.02 ± 0.07 mg/ml). The plant extracts were characterized by UV-visible spectroscopy, Fourier-transform infrared spectroscopy, and LC-MS/MS.

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Section: Introductionmentioning

confidence: 99%

“…3 Numerous pharmaceuticals originate from plant extracts and have been the subject of extensive pharmacological screening. 1 Plants contain an array of secondary metabolites that play crucial roles in their survival and adaptation to the environment. These compounds belong to three major classes, namely, alkaloids, phenols, and terpenoids.…”

Section: Introductionmentioning

confidence: 99%