Understanding stenosis-induced platelet aggregation on a chip by high-speed optical imaging

Studying the efficacy of antiplatelet drugs on atherosclerosis by optofluidic imaging on a chip

“…Since platelet activation and aggregation can be induced not only by stenosis but also by agonists, we further applied our method to evaluate the efficacy of antiplatelet drugs by combining both stenosis (biomechanical factor) and agonists (biochemical factor) for inducing platelet activation. Previously, we have reported that there is a synergistic effect of stenosis and agonists on platelet activation and aggregation, 43 which seemingly pose further challenges to the efficacy of antiplatelet drugs for patients with atherosclerosis. Here, we used ADP and collagen, which are two commonly used agonists in clinical experiments, to activate platelets prior to perfusing through the stenosis chip.…”

Valve-Adjustable Optofluidic Bio-Imaging Platform for Progressive Stenosis Investigation

“…Microfluidic bionic stenosis platforms have gained great concern in virtue of their on-demand design structure, excellent visualization, high integration properties, and contribution to a series of investigation ranging from mechanism investigation, pharmaceutical development, and therapeutics of valvular stenosis-related diseases. − The developed microfluidic platforms are mainly based on in situ microfluidic valve design, ,− photocurable hydrogel printing, etc. − For example, Chen et al. developed a microfluidic model with in situ designed stenosis for biomechanical platelet aggregation investigation .…”

“…In addition, the model is required to make accurate recognition and quantitative analysis with the interferences of multiple scattering in blood cells, motion blur, etc., in complex blood flow imaging. , Nevertheless, the current paradigm of sensing by simple optical microscopes with limited resolution and performance could not meet these requirements. In response, a number of good systems with a delicate optical system − have been proposed, but the complexity of necessary devices limits their widespread popularity. The investigation necessitates the development of methodology and bioengineering technologies that ensures the valvular tunability of progressive valvular stenosis state bio-simulation and good compatible optical imaging analysis.…”