Understanding the immune system architecture and transcriptome responses to southern rice black-streaked dwarf virus in Sogatella furcifera

Wang, Lin; Tang, Nan; Gao, Xinlei; Guo, Dongyang; Chang, Zhaoxia; Fu, Yating; Akinyemi, Ibukun A.; Wu, Qingfa

doi:10.1038/srep36254

Cited by 31 publications

(48 citation statements)

References 57 publications

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“…Furthermore, at the whole transcriptome level, virus infection can also alter the small RNA profiles of the host. Some small RNAs could be induced or exploited by viruses to favor the viral infection and persistence by attenuating the immune response, ceasing cell apoptosis, and promoting cell growth [52][53][54]. Interestingly, this strategy, as employed by a virus, was observed in HSV-1 and its host interactions [55][56][57].…”

Section: Discussionmentioning

confidence: 99%

Deep RNA Sequencing Reveals a Repertoire of Human Fibroblast Circular RNAs Associated with Cellular Responses to Herpes Simplex Virus 1 Infection

Shi¹,

Hu²,

Mo³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs), a new class of regulators of gene expression, are involved in diverse physiological and pathogenic processes. However, their role in cellular responses to virus infection is yet unclear. Methods: A human lung fibroblast cell line was infected or mock infected by herpes simplex virus 1 (HSV-1). Deep RNA sequencing was used to profile the global changes in circRNAs, genes, and miRNAs following HSV-1 infection. Altered circRNAs, genes, and miRNAs were validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR). An integration analysis of circRNAs, genes, and miRNAs was applied to investigate the putative function of the dysregulated circRNAs. Results: A total of 536 circRNAs, 3,885 genes, and 207 miRNAs were significantly dysregulated after HSV-1 infection. An integration analysis of circRNAs, genes, and miRNAs revealed the alleged involvement of dysregulated circRNAs in cellular responses to HSV-1 infection via the circRNA-miRNA-gene regulatory axis. These genes regulated by circRNAs were enriched to NOD-like receptor/JAK-STAT signaling pathway, and pathways of apoptosis, cell cycle progression, and cell death, all of which may be implicated in the viral pathogenesis and cellular immunity. Conclusions: These data present a comprehensive view for circRNAs induced by HSV-1 and their interplay with miRNAs and genes during HSV-1 infection, thus offering new insights into the mechanisms of interactions between HSV-1 and the host.

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Section: Discussionmentioning

confidence: 99%

Deep RNA Sequencing Reveals a Repertoire of Human Fibroblast Circular RNAs Associated with Cellular Responses to Herpes Simplex Virus 1 Infection

Shi¹,

Hu²,

Mo³

et al. 2018

Cell Physiol Biochem

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“…The sequencing data were retrieved from the work of Wang et al . (2016a). The extended 3′‐terminal sequences in RNA segments 4–8 are marked in red.…”

Section: Resultsmentioning

confidence: 99%

“…SRBSDV is a double‐stranded, multiple‐segment RNA virus that infects a variety of plants, such as rice and maize (Zhou et al ., 2013). The genome of SRBSDV contains 10 segments that are named S1–S10 (Wang et al ., 2016a). We de novo assembled the SRBSDV genome using the transcriptomic sequencing data that were generated from cDNA libraries constructed using random primers (Wang et al ., 2016a).…”

Section: Resultsmentioning

confidence: 99%

“…The genome of SRBSDV contains 10 segments that are named S1–S10 (Wang et al ., 2016a). We de novo assembled the SRBSDV genome using the transcriptomic sequencing data that were generated from cDNA libraries constructed using random primers (Wang et al ., 2016a). Compared with the published reference genome (from NC_014708.1 to NC_014717.1 in GenBank), the de novo assembled SRBSDV genome contained 3′‐terminal extension sequences of 13 to 17 nt in length on RNA segments 4–8, and each extension sequence was identical or nearly identical to a fragment in the upstream region of the same RNA segment (Figs 3b, S4).…”

Section: Resultsmentioning

confidence: 99%

“…The SRBSDV genome sequencing data were retrieved from the published work of Wang et al (2016a). Two versions of the genome were assembled with SRBSDV reads from the viruliferous strains with high viral titres (HVT) and medium viral titres (MVT), and the resulting genome assemblies were compared with the published reference genome from GenBank (from NC_ 014708.1 to NC_014717.1) using the same methods as those used in the RSV genome assembly and comparison.…”

Section: Researchmentioning

confidence: 99%

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Genomic variations in the 3′‐termini of Rice stripe virus in the rotation between vector insect and host plant

Zhao

Zhang

et al. 2018

New Phytologist

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Summary A large number of plant RNA viruses circulate between plants and insects. For RNA viruses, host alternations may impose a differential selective pressure on viral populations and induce variations in viral genomes. Here, we report the variations in the 3′‐terminal regions of the multiple‐segment RNA virus Rice stripe virus (RSV) that were discovered through de novo assembly of the genome using RNA sequencing data from infected host plants and vector insects.The newly assembled RSV genome contained 16‐ and 15‐nt extensions at the 3′‐termini of two genome segments compared with the published reference RSV genome. Our study demonstrated that these extensional sequences were consistently observed in two RSV isolates belonging to distinct genetic subtypes in RSV‐infected rice, wheat and tobacco. Moreover, the de novo assembled genome of Southern rice black‐streaked dwarf virus also contained 3′‐terminal extensions in five RNA segments compared with the reference genome.Time course experiments confirmed that the 3′‐terminal extensions of RSV were enriched in the vector insects, were gradually eliminated in the host plant and potentially affected viral replication.These findings indicate that variations in the 3′‐termini of viral genomes may be different adaptive strategies for plant RNA viruses in insects and plants.

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Crucial role of a takeout protein in white‐backed planthopper Sogatella furcifera (Horváth) orientation towards its host rice plants

He,

Long,

Feng

et al. 2024

Pest Management Science

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The takeout (TO) gene family impacts diverse physiological and behavioral functions in insects, yet specific olfactory‐associated roles for the family have yet to be fully elucidated. To provide insights into TO function in rice planthoppers, the genomes of three rice planthoppers (white‐backed planthopper, brown planthopper and small brown planthopper) were searched for TO homologs and their degree of conservation assessed via chromosomal localization, exon–intron boundaries, phylogenetic relationships and protein domains/motifs. We performed a tissue‐specific expression analysis of the 20 TO genes in the white‐backed planthopper (WBPH, Sogatella furcifera) and found that SfTO17 is enriched in adult antennae. RNAi‐mediated knockdown of SfTO17 impaired WBPH olfaction and reduced host‐seeking responses following exposure to rice plants. The binding profile of β‐ionone, hexyl benzoate and benzyl benzoate with recombinant SfTO17 was evaluated via competitive fluorescence binding assays. Conformational prediction of SfTO17 coupled with molecular docking analyses revealed several amino acid residues potentially critical for odorant binding. This study demonstrates the olfactory function of SfTO17 in WBPH and highlights its potential as a target for controlling this rice pest. © 2024 Society of Chemical Industry.

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Understanding the immune system architecture and transcriptome responses to southern rice black-streaked dwarf virus in Sogatella furcifera

Cited by 31 publications

References 57 publications

Deep RNA Sequencing Reveals a Repertoire of Human Fibroblast Circular RNAs Associated with Cellular Responses to Herpes Simplex Virus 1 Infection

Deep RNA Sequencing Reveals a Repertoire of Human Fibroblast Circular RNAs Associated with Cellular Responses to Herpes Simplex Virus 1 Infection

Genomic variations in the 3′‐termini of Rice stripe virus in the rotation between vector insect and host plant

Crucial role of a takeout protein in white‐backed planthopper Sogatella furcifera (Horváth) orientation towards its host rice plants

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