Understanding the mechanism of replacement of citrate from the surface of gold nanoparticles by amino acids: a theoretical and experimental investigation and their biological application

Rani, Monika; Moudgil, Lovika; Singh, Baljinder; Kaushal, Akshey; Mittal, Anu; Saini, G. S. S.; Tripathi, S. K.; Singh, Gurinder; Kaura, Aman

doi:10.1039/c5ra26502a

Cited by 36 publications

(22 citation statements)

References 48 publications

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“…For example, nanoparticles prepared by the approach described by Lee and Meisel are stabilized by citrate, and such stabilization is based on the electrostatic forces. Adsorption of analyte molecules can occur on the surface of citrate‐stabilized nanoparticles by substitution of citrate molecules on the silver surface or by the interaction of the analyte molecules with the citrate molecules . Another method for stabilization of nanoparticles based on steric polymer repulsion is also possible.…”

Section: Methodsmentioning

confidence: 99%

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Adomaviciute

Velička

Šablinskas

2020

J Raman Spectroscopy

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Aspirin being freely available as an over the counter drug has a high overdose risk and can cause severe health symptoms. The methods used in clinics for the detection of such drugs (pharmaceuticals) in bodily fluids are mostly based on liquid chromatography and mass spectroscopy. These methods are known to be precise; however, they both require long and laborious sample preparation; thus, it takes time to acquire the required information. Because in the case of an overdose, the time has high importance, and faster methods would be beneficial. This work presents an application of a Surface‐enhanced Raman scattering (SERS) spectroscopic method for the detection of salicylic acid as a metabolite of aspirin in the blood serum of the patient under examination. In this research, the various colloidal solutions were employed for the preparation of SERS active substrates. The choice of the most efficient colloidal solution and the challenges of collecting SERS spectra of whole blood or its components are discussed. The spectra of both the model and the real‐life blood samples containing metabolite of aspirin were collected with a Fourier transform Raman spectrometer. The analysis of the collected spectra revealed that label‐free SERS can be used for the detection of salicylic acid with concentration down to 3 mM in blood serum corresponding to consumption of at least eight standard pills of aspirin (equivalent to mild toxicity). The proposed diagnostic method could be applied faster than the standard methods and could allow sensitive and fast diagnosis of aspirin consumption in the human blood.

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Section: Methodsmentioning

confidence: 99%

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Adomaviciute

Velička

Šablinskas

2020

J Raman Spectroscopy

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“…There are sufficient reports indicating the safety profile of AuNPs in terms of negligible to very low cytotoxicity to various host cells/cell lines and hemolysis 43–45 . A recent study has reported that coating of amino acids on the surface of AuNPs minimizes the chance of coming in contact with RBCs, hence results in reduced hemolysis 49 thus further supporting non-toxic and biocompatible nature of nanoformulations of Pep-H.…”

Section: Discussionmentioning

confidence: 81%

Exploiting chitosan and gold nanoparticles for antimycobacterial activity of in silico identified antimicrobial motif of human neutrophil peptide-1

Sharma

Raghav

Kumari

et al. 2019

Sci Rep

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The upsurge of drug resistant tuberculosis is major health threat globally. To counteract, antimicrobial peptides are being explored as possible alternatives. However, certain limitations of peptide-based drugs such as potential toxicity, high cost and relatively low stability need to be addressed to enhance their clinical applicability. Use of computer predicted short active motifs of AMPs along with nanotechnology could not only overcome the limitations of AMPs but also potentiate their antimicrobial activity. Therefore, present study was proposed to in silico identify short antimicrobial motif (Pep-H) of human neutrophil peptide-1 (HNP-1) and explore its antimycobacterial activity in free form and using nanoparticles-based delivery systems. Based on colony forming unit analysis, motif Pep-H led to killing of more than 90% M. tb in vitro at 10 μg/ml, whereas, similar activity against intracellularly growing M. tb was observed at 5 μg/ml only. Thereafter, chitosan (244 nm) and gold nanoparticles (20 nm) were prepared for Pep-H with both the formulations showing minimal effects on the viability of human monocyte derived macrophages (MDMs) and RBC integrity. The antimycobacterial activity of Pep-H against intracellular mycobacteria was enhanced in both the nanoformulations as evident by significant reduction in CFU (>90%) at 5–10 times lower concentrations than that observed for free Pep-H. Thus, Pep-H is an effective antimycobacterial motif of HNP-1 and its activity is further enhanced by chitosan and gold nanoformulations.

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“…Gold nanoparticles (AuNPs) have gained considerable popularity as one of the most stable metal nanoparticles with unique electrical, optical and surface chemical properties making them applicable in fields such as sensing, drug delivery, cancer therapy and tissue engineering [1][2][3][4] . It is well known that AuNPs can be synthesized using different chemical reduction methods; out of which the citrate method introduced by Turkevich is the most widely used for the preparation of AuNPs 5,6 The biocompatible nature of citrate ion and its ability to act as a cross-linker between various ligands along with its labile nature with respect to displacement by various ligands on the surface of AuNPs render Turkevich method as one of the most popular for AuNPs synthesis 7,8 . However, in spite of the popularity and extensive usage of citrate based methods for synthesis of AuNPs 9,10 , there has been limited introspection in literature into the probable interaction between citrate and gold ions along with the structural details of citrate adsorption.…”

Section: Quantification Of Adsorbed and Dangling Citrate Ions On Goldmentioning

confidence: 99%

Quantification of adsorbed and dangling citrate ions on gold nanoparticle surface using thermogravimetric analysis

Bajaj

Wangoo

Jain

et al. 2020

Sci Rep

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A novel approach involving thermo-gravimetricanalysis (TGA) for the quantification of citrate ions present on the surface of gold nanoparticles has been reported. TGA study was carried out on AuNPs in response to parameters such as concentration of tri-sodium citrate and pH of gold nanoparticles depicting that the number of citrate ion present on gold nanoparticles is highly pH dependent. In general, the citrate ions were observed to be higher in alkaline conditions contradicting earlier beliefs. These results also underline the significance of TGA as a novel tool for quantification of citrate molecules present on gold nanoparticle surface. Thus, the present approach not only provides with an insight into mechanistic details of gold nanoparticle synthesis but also opens the usage of TGA for understanding the nano range association of molecules.

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Understanding the mechanism of replacement of citrate from the surface of gold nanoparticles by amino acids: a theoretical and experimental investigation and their biological application

Abstract: The present study explores the physicochemical aspects needed for the appropriate in vitro synthesis and surface modification behavior of gold nanoparticles (AuNPs) in the presence of amino acids (AA).

Cited by 36 publications

References 48 publications

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Detection of aspirin traces in blood by means of surface‐enhanced Raman scattering spectroscopy

Exploiting chitosan and gold nanoparticles for antimycobacterial activity of in silico identified antimicrobial motif of human neutrophil peptide-1

Quantification of adsorbed and dangling citrate ions on gold nanoparticle surface using thermogravimetric analysis

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