Understanding the molecular pathways associated with seed vigor

Ventura, Lorenzo; Donà, Mattia; Macovei, Anca; Carbonera, Daniela; Buttafava, A.; Mondoni, Andrea; Rossi, Graziano; Balestrazzi, Alma

doi:10.1016/j.plaphy.2012.07.031

Cited by 152 publications

(115 citation statements)

References 83 publications

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“…The maturation phase is classically associated with a progressive decline in the base metabolism, a decrease in the hydration level of the seed, and acquisition of new competencies such as desiccation tolerance (Holdsworth et al, 2008). Seed maturation involves regulating events that integrate genetic attributes, and metabolic and hormonal signs in order to induce dormancy, a state of rest, that allows the seeds to survive in an environment (Ventura et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity

Santos¹,

Caixeta²,

Clemente³

et al. 2014

Genet. Mol. Res.

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ABSTRACT.Seeds collected at different maturation stages vary in physiological quality and patterns of protective antioxidant systems against deterioration. In this study we investigated the expression of genes that codify catalase (CAT), dismutase superoxide (SOD), and polyphenol oxidase (PPO) during the pre-and post-physiological maturation phases in whole seeds and in endosperms and embryos extracted from the seeds. Coffea arabica L. berries were collected at the green, yellowish-green, cherry, over-ripe, and dry stages, and the seeds were examined physiologically. The transcription levels of the genes were quantified by quantitative real-time polymerase chain reaction using coffee-specific primers. The highest level of SOD expression was observed in the endosperm at the cherry and over-ripe stages; in addition, these seeds presented the greatest physiological quality (assessed via germination test). The highest CAT3 transcript expression was observed at the green stage in whole seeds, and at the green and over-ripe stages in the embryos and endosperms. High expression of the PPO transcript was observed at the green and yellowish-green stages in whole seeds. In embryos and endosperms, peak expression of the PPO transcript was observed at the green stage; subsequently, peaks at the cherry and over-ripe stages were observed. We concluded that the expression patterns of the SOD and CAT3 transcripts were similar at the more advanced maturation stages, which corresponded to enhanced physiological seed quality. High expression of the PPO transcript at the over-ripe stage, also observed in the embryos and endosperms at the cherry stage, coincided with the highest physiological seed quality.

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Section: Discussionmentioning

confidence: 99%

Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity

Santos¹,

Caixeta²,

Clemente³

et al. 2014

Genet. Mol. Res.

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“…It has been identified by numerous inclusive reviews: reactive oxygen species, lipid peroxidation mediated by free radicals, imbalances in growth-regulating enzymes, enzyme activation or protein degradation, posttranslational modification of proteins, impairment of metabolic transition, damage to genetic integrity, and commotion of cellular membranes as main factors of seed aging (Priestly, 1986;Smith and Berjak, 1995;Walters, 1998;McDonald, 1999;Ventura et al, 2012;Rajjou et al, 2012; Table 1). …”

Section: Aim Of This Reviewmentioning

confidence: 99%

“…Following double fertilization, both the embryo and storage tissue begin to grow and continue to grow until a specific time, which depends on the particular plant species. When the development of the embryo is repressed on the mother plant and storage products are collected, by the aid of compound regulatory complexes that incorporate genetic programs, metabolic signals and pathways for hormonal signaling, seed maturation starts to acquire tolerance against desiccation (Ventura et al, 2012). However, not all seeds can withstand desiccation, and only orthodox seeds can be stored ex situ in their dehydrated state for long periods, which is regulated by complex networks of molecular and biochemical processes.…”

Section: Aim Of This Reviewmentioning

confidence: 99%

“…One important regulatory mechanism is the posttranslational modifications of histones, which ensures the appropriate chromatin structure (Ventura et al, 2012). In Arabidopsis thaliana, the HUB1 (Histone Monoubiquitination) and HUB2 genes encode E3 ligases, which are necessary for the monoubiquitination of histone H2B.…”

Section: Associated Changes Effects Referencesmentioning

confidence: 99%

“…Thus, a variety of cellular, molecular, and physiological processes contribute to seeds' ability to withstand desiccation and to resume germination under favorable conditions. describe the progress in the field of research (for detail see Bove et al, 2001;Chaudhury et al, 2001;Weber et al, 2005;Le et al, 2007;Linkies et al, 2010;Nonogaki et al, 2010;Rajjou et al, 2012;Ventura et al, 2012;Sreenivasulu and Wobus, 2013).…”

Section: Associated Changes Effects Referencesmentioning

confidence: 99%

See 2 more Smart Citations

Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

Ahmad¹,

Shah²,

Rehman³

et al. 2017

Current Issues in Molecular Biology

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Abbreviations:AFLP, amplified fragment length polymorphism AGO, argonaute miRNA, micro RNA NGS, next generation sequencing PCD, programmed cell death PIMT, protein L-isoaspartyl methyltransferase PRC2, Polycomb repressive complex 2 RAPD, random amplification of polymorphic DNA ROS, reactive oxygen species SSR, simple sequence repeat TUNEL, terminal deoxynucleotide transferasemediated dUTP nick-end labeling qRT-PCR, quantitative reverse transcriptional polymerase chain reaction Abstract For human food security, the preservation of 7.4 million ex-situ germplasm is a global priority. However, ex situ-conserved seeds are subject to aging, which reduces their viability and ultimately results in the loss of valuable genetic material over long periods. Recent progress in seed biology and genomics has revealed new opportunities to improve the long-term storage of ex-situ seed germplasm. This review summarizes the recent improvements in seed physiology and genomics, with the intention of developing genomic tools for evaluating seed aging. Several lines of seed biology research have shown promise in retrieving viability signal from various stages of seed germination. We conclude that seed aging is associated with mitochondrial alteration and programmed cell death, DNA and enzyme repair, anti-oxidative genes, telomere length, and epigenetic regulation. Clearly, opportunities exist for observing seed aging for developing genomic tools to increment the traditional germination test for effective conservation of ex-situ germplasm.

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Medicago truncatula, an informative model to investigate theDNAdamage response during seed germination

Macovei

Pagano

Forti

et al. 2019

The Model Legume Medicago Truncatula

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Understanding the molecular pathways associated with seed vigor

Cited by 152 publications

References 83 publications

Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity

Gene expression of antioxidant enzymes and coffee seed quality during pre- and post-physiological maturity

Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

Medicago truncatula, an informative model to investigate theDNAdamage response during seed germination

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