Uneven drying of zygotic embryos and embryonic axes of recalcitrant seeds: Challenges and considerations for cryopreservation

Ballesteros, Daniel; Sershen,; Varghese, Boby; Berjak, Patricia; Pammenter, N. W.

doi:10.1016/j.cryobiol.2014.05.010

Cited by 29 publications

(40 citation statements)

References 27 publications

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“…; Ballesteros et al . ). Additionally, a further consideration when designing this study was that the effect of a stress in desiccation‐sensitive tissues, particularly a mild stress, is unlikely to be instantaneous.…”

Section: Methodsmentioning

confidence: 97%

“…Given the fact that recalcitrant seeds of different species can be shed at significantly different embryo WCs (Ballesteros et al . ), the embryo WCs after flash drying were normalised by calculating percentage water loss according to the following formula:

% Water loss = \frac{Shedding WC - WC after drying}{Shedding WC} \times 100 .

…”

Section: Methodsmentioning

confidence: 99%

“…In this regard, some success has been achieved using excised zygotic embryos, embryonic axes (Ballesteros et al . ) and plumules (apical meristems isolated from shoot axis; Chmielarz et al . ) for cryopreservation, provided that, prior to cooling, they are dried to WCs that minimise lethal intracellular ice crystal formation during cooling (Normah et al .…”

Section: Introductionmentioning

confidence: 99%

“…; Ballesteros et al . ) since desiccation damage in recalcitrant seeds appears to be a function of two interrelated parameters: the degree and the duration of dehydration (Pammenter et al . , ; Varghese et al .…”

Section: Introductionmentioning

confidence: 99%

“…; Ballesteros et al . ), and/or accompanied by a high incidence of ‘abnormal’ growth ( i.e., no roots, no shoots or only callus; e.g ., Sershen et al . ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The use of plant stress biomarkers in assessing the effects of desiccation in zygotic embryos from recalcitrant seeds: challenges and considerations

et al. 2016

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Zygotic embryos from recalcitrant seeds are sensitive to desiccation. In spite of their sensitivity, rapid partial dehydration is necessary for their successful cryopreservation. However, dehydration to water contents (WCs) that preclude lethal ice crystal formation during cooling and rewarming generally leads to desiccation damage. This study investigated the effects of rapid dehydration on selected stress biomarkers (electrolyte leakage, respiratory competence, rate of protein synthesis, superoxide production, lipid peroxidation, antioxidant activity and degree of cellular vacuolation) in zygotic embryos of four recalcitrant-seeded species. Most biomarkers indicated differences in the levels of stress/damage incurred by embryos dried to WCs < and >0.4 g·g(-1) , within species; however, these changes were often unrelated to viability and percentage water loss when data for the four species were pooled for regression analyses. Dehydration-induced electrolyte leakage was, however, positively related with percentage water loss, while biomarkers of cellular vacuolation were positively related with both percentage water loss and viability. This suggests that electrolyte leakage and degree of cellular vacuolation can be used to quantify dehydration-induced stress/damage. Biomarkers such as superoxide production, whilst useful in establishing the nature of the dehydration stress incurred may not be able to distinguish the effects of different WCs/drying times. Irrespective of which biomarker is used, the data suggest that understanding differences in desiccation sensitivity across recalcitrant-seeded species will remain a challenge unless these biomarkers are related to a generic desiccation stress index that integrates the effects of percentage water loss and drying time.

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Section: Methodsmentioning

confidence: 97%

% Water loss = \frac{Shedding WC - WC after drying}{Shedding WC} \times 100 .

…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…; Ballesteros et al . ), and/or accompanied by a high incidence of ‘abnormal’ growth ( i.e., no roots, no shoots or only callus; e.g ., Sershen et al . ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The use of plant stress biomarkers in assessing the effects of desiccation in zygotic embryos from recalcitrant seeds: challenges and considerations

et al. 2016

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Conservation of Hardwood Forest Species

Corredoira

Martínez

San-José

et al. 2017

Sustainable Development and Biodiversity

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Impact of drying and cooling rate on the survival of the desiccation-sensitive wheat pollen

2022

Self Cite

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Key message Fast-drying and cooling induce fast intracellular water loss and reduced ice-crystal formation, which may promote the formation of intracellular glasses that might improve the likelihood of wheat pollen survival. Abstract Long-term storage of pollen is important for the fertilization of spatially or temporally isolated female parents, especially in hybrid breeding. Wheat pollen is dehydration-sensitive and rapidly loses viability after shedding. To preserve wheat pollen, we hypothesized that fast-drying and cooling rates would increase the rate of intracellular water content (WC) removal, decrease intracellular ice-crystal formation, and increase viability after exposure to ultra-low temperatures. Therefore, we compared slow air-drying with fast-drying (dry air flow) and found significant correlations between pollen WC and viability (r = 0.92, P < 0.001); significant differences in WCs after specific drying times; and comparable viabilities after drying to specific WCs. Fast-drying to WCs at which ice melting events were not detected (ΔH = 0 J mg−1 DW, < 0.28 mg H2O mg−1 DW) reduced pollen viability to 1.2 ± 1.0%, but when drying to 0.39 mg H2O mg−1 DW, some viable pollen was detected (39.4 ± 17.9%). Fast cooling (150 °C min−1) of fast-dried pollen to 0.91 ± 0.11 mg H2O mg−1 DW induced less and a delay of ice-crystal formation during cryomicroscopic-video-recordings compared to slow cooling (1 °C min−1), but viability was low (4.5–6.1%) and comparable between cooling rates. Our data support that the combination of fast-drying and cooling rates may enable the survival of wheat pollen likely due to (1) a reduction of the time pollen would be exposed to drying-related deleterious biochemical changes and (2) an inhibition of intracellular ice-crystal formation, but additional research is needed to obtain higher pollen survival after cooling.

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Uneven drying of zygotic embryos and embryonic axes of recalcitrant seeds: Challenges and considerations for cryopreservation

Cited by 29 publications

References 27 publications

The use of plant stress biomarkers in assessing the effects of desiccation in zygotic embryos from recalcitrant seeds: challenges and considerations

The use of plant stress biomarkers in assessing the effects of desiccation in zygotic embryos from recalcitrant seeds: challenges and considerations

Conservation of Hardwood Forest Species

Impact of drying and cooling rate on the survival of the desiccation-sensitive wheat pollen

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