2007
DOI: 10.1103/physrevlett.98.158101
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Unfolding of Proteins and Long Transient Conformations Detected by Single Nanopore Recording

Abstract: We study the electrophoretic blockades due to entries of partially unfolded proteins into a nanopore as a function of the concentration of the denaturing agent. Short and long pore blockades are observed by electrical detection. Short blockades are due to the passage of completely unfolded proteins, their frequency increases as the concentration of the denaturing agent increases, following a sigmoidal denaturation curve. Long blockades reveal partially folded conformations. Their duration increases as the prot… Show more

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Cited by 272 publications
(385 citation statements)
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“…3,11,21,23,[28][29][30] Several biophysical parameters have been determined such as threading frequency, [31][32][33][34] orientation of strands, [35][36][37][38][39] velocity of DNA transport, 40 influence of pore geometry, 41 and interactions with the pore wall. 39 In addition to nucleic acids, translocation has also been investigated for peptides, [42][43][44][45] proteins, [46][47][48] and peptide-oligonucleotide conjugates, 49 which, unlike nucleic acid strands, frequently feature inhomogeneous charge distributions and vary considerably in diameter along the polymer sequence.…”
Section: Introductionmentioning
confidence: 99%
“…3,11,21,23,[28][29][30] Several biophysical parameters have been determined such as threading frequency, [31][32][33][34] orientation of strands, [35][36][37][38][39] velocity of DNA transport, 40 influence of pore geometry, 41 and interactions with the pore wall. 39 In addition to nucleic acids, translocation has also been investigated for peptides, [42][43][44][45] proteins, [46][47][48] and peptide-oligonucleotide conjugates, 49 which, unlike nucleic acid strands, frequently feature inhomogeneous charge distributions and vary considerably in diameter along the polymer sequence.…”
Section: Introductionmentioning
confidence: 99%
“…38 Thus the electronic signal at the instant that the protein interacts with the pore is very sensitive to the protein's structure and conformation. [39][40][41] A complete profile for wild-type bovine prion protein bPrP, could be accumulated in about 3 h (Fig. 1A).…”
Section: Resultsmentioning
confidence: 99%
“…La détection du passage de ces molécules est réalisée par la mesure du courant ionique traversant le pore : un pore vide correspond à un courant élevé ; La présence d'une macromolécule, telle que l'ADN, réduit le flux d'ions au travers du pore, et permet de détecter le passage d'une molécule individuelle (Figure 1, encart haut). Cette méthode a, dès lors, été appliquée à la détection d'autres macromolécules (le polyéthylène glycol [PEG] [7] ou des protéines [8,9]), à la caractérisation de la distribution de masse d'un polymère [7], mais aussi à l'étude de la conformation de macromolécules (structures secondaires et tertiaires [8,10], noeuds [11], association spécifique de protéines [12]). Pour pouvoir identifier chacune des paires de base de la molécule transportée dans le nanopore, il a fallu résoudre de nombreuses difficultés techniques et améliorer fortement les performances du système.…”
Section: Séquençage De L'adn Par Nanopores Résultats Et Perspectivesunclassified