Uniform Biodegradable Hydrogel Microspheres Fabricated by a Surfactant‐Free Electric‐Field‐Assisted Method

Choy, Young Bin; Choi, Hyungsoo; Kim, Kyekyoon Kevin

doi:10.1002/mabi.200700020

Cited by 18 publications

(6 citation statements)

References 47 publications

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“…Gelatin (225 g bloom, BioReagent) nanoparticles were fabricated by an E-PPF method [18] and were cross-linked using 200 mL of 0.125, 0.375, 0.625, and 0.875% w/v glutaraldehyde (GA) (25% aqueous solution, Sigma-Aldrich) solutions at 4 °C for 24 hours, followed by the addition of glycine (Sigma-Aldrich) at room temperature to deactivate the remaining GA [17]. The resulting nanoparticles were washed with DI water and lyophilized.…”

Section: Methodsmentioning

confidence: 99%

“…1. The nanoparticle core was fabricated by the Electric Field Assisted Precision Particle Fabrication (E-PPF) method using acidic gelatin, loaded with DOX [17], [18]. The resulting nanospheres were coated with a high-density peptide layer, the hydrolysis of which is catalyzed by Cathepsin D, a specific biomarker protease hypersecreted by breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro and In Vivo Imaging of Peptide-Encapsulated Polymer Nanoparticles for Cancer Biomarker Activated Drug Delivery

Kulsharova

Lee

Cheng

et al. 2013

IEEE Trans.on Nanobioscience

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Gelatin nanoparticles coated with Cathepsin D-specific peptides were developed as a vehicle for the targeted delivery of the cancer drug doxorubicin (DOX) to treat breast malignancy. Cathepsin D, a breast cancer cell secretion enzyme, triggered the release of DOX by digesting the protective peptide-coating layer of nanoparticles. Fabricated nanoparticles were successfully detected with ultrasound imaging in both in vitro conditions and in vivo mouse cancer models. Cell viability experiments were conducted to determine the efficacy of biomarker activation specific to breast cancer cell lines. These experimental results were compared with the outcome of a viability experiment conducted on noncancerous cells. Viability decreased in human MCF7 mammary adenocarcinoma and mouse 4T1 mammary carcinoma cells, while that of noncancerous 3T3 fibroblast cells remained unaffected. Next, a real-time video of nanoparticle flow in mouse models was obtained using in vivo ultrasound imaging. The fluorescent profile of DOX was used as a means to examine nanoparticle localization in vivo. Results show the distribution of nanoparticles concentrated primarily within bladder and tumor sites of subject mice bodies. These findings support the use of biomarker coated nanoparticles in target specific therapy for breast cancer treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Imaging of Peptide-Encapsulated Polymer Nanoparticles for Cancer Biomarker Activated Drug Delivery

Kulsharova

Lee

Cheng

et al. 2013

IEEE Trans.on Nanobioscience

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“…The electrostatic atomization is a technique for the atomization of liquid with an electrohydrodynamic (EHD) force, and many studies are reported in recent years . The electrostatic atomization could be classified into three systems as follows: (1) a liquid dispersion into a gas (L/G) system, (2) gas dispersion into a liquid (G/L) system, and (3) a liquid dispersion into a liquid (L/L) system.…”

Section: Introductionmentioning

confidence: 99%

“…According to these reports, chitosan or chitosan‐tripolyphosphate microspheres cross‐linked with glutaraldehyde were prepared with a uniform size of about 5 µm, containing bovine serum albumin or insulin. Finally, Choy et al , reported the chitosan‐based microspheres, which has uniform size of 10–60 µm, could be prepared by using double‐tube nozzle in electric field . Many of recent studies on chitosan‐based nano‐particles for drug delivery were mentioned in a review article …”

Section: Introductionmentioning

confidence: 99%

Chitosan nano‐spheres production by electrostatic emulsification technique

Takahashi

Itobayashi

Shono

et al. 2011

Asia-Pacific J Chem Eng

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Electrostatic emulsification of an acidic chitosan solution into an organic solvent was examined for chitosan sphere production of controlled size ranging from nano to submicron scale. Droplets formation by the electrostatic emulsification was observed with a charge-coupled device camera. The diameters of generated water in oil emulsion droplets were measured by dynamic light scattering. At voltages greater than critical voltage, over which the electrostatic emulsification occurs, the droplet diameter "D" depended on the applied voltage "U", the electrode distance "L", and the flow rate of aqueous phase "F". An empirical formula for the relationship between the "D" and "U", "L" and "F" was obtained as D/U À0.65 L 0.43 F 0.14 . Further, the chitosan spheres were obtained by cross-linking the emulsion droplets with glutaraldehyde, and were observed by using a scanning electron microscope. The chitosan nano-spheres of the size equal to the emulsion droplet were successfully obtained by the cross-linking of the chitosan and controlling a specific gravity difference of dispersed phase and continuous phase. These results show that the electrostatic emulsification is an excellent technique for the preparation of functional particles of controlled size ranging from nano to submicron scale.

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“…Previously, we reported that mucoadhesive microparticles, when delivered by way of a mannitol-based tablet, resided on the preocular surface longer than the other formulations tested (i.e., aqueous suspensions and tablets with non-mucoadhesive microparticles). 20,21 The prolonged residence time of microparticles was ascribed to the fact that, unlike suspensions, a rapidly dissolving tablet would not expedite tear drainage and, thus, facilitate initial contact between mucoadhesive microparticles and the preocular mucus surface.…”

mentioning

confidence: 99%

Mucoadhesive Microparticles in a Rapidly Dissolving Tablet for Sustained Drug Delivery to the Eye

Choy

Patel

Park

et al. 2011

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To test the hypothesis that mucoadhesive microparticles formulated in a rapidly dissolving tablet can achieve sustained drug delivery to the eye. METHODS. Mucoadhesive microparticles, smaller than 5 m were fabricated with poly(lactic-co-glycolic acid) and poly(ethylene glycol) as a core material and mucoadhesion promoter, respectively, and encapsulated pilocarpine as a model drug. These microparticles were embedded in a poly(vinyl alcohol) matrix to form a dry tablet designed to reduce rapid clearance of the microparticles on initial application to the eye. RESULTS. This in vitro drug release study exhibited that for all formulations, approximately 90% of pilocarpine was released during the first 10 minutes, and the remaining 10% was released slowly for 3 hours. In vivo mucoadhesion test on the rabbit eye indicated that mucoadhesive microparticles adhered significantly better to the preocular surface than other formulations. To assess the pharmacodynamics, the most prolonged pilocarpine-induced pupil constriction was observed in rabbit eyes in vivo using a tablet with mucoadhesive microparticles; it lasted up to 330 minutes. CONCLUSIONS. The authors conclude that mucoadhesive microparticles formulated into a dry dosage form is a promising system for sustained drug delivery to the eye. (Invest Ophthalmol Vis Sci.

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Uniform Biodegradable Hydrogel Microspheres Fabricated by a Surfactant‐Free Electric‐Field‐Assisted Method

Cited by 18 publications

References 47 publications

In Vitro and In Vivo Imaging of Peptide-Encapsulated Polymer Nanoparticles for Cancer Biomarker Activated Drug Delivery

In Vitro and In Vivo Imaging of Peptide-Encapsulated Polymer Nanoparticles for Cancer Biomarker Activated Drug Delivery

Chitosan nano‐spheres production by electrostatic emulsification technique

Mucoadhesive Microparticles in a Rapidly Dissolving Tablet for Sustained Drug Delivery to the Eye

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