Uniform RNA Patterns of Beet Necrotic Yellow Vein Virus in Sugarbeet Roots, but Not in Leaves from Several Plant Species

Koenig, R.; Burgermeister, W.; Weich, Herbert A.; Sebald, Walter; Kothe, Caroline Isabel

doi:10.1099/0022-1317-67-9-2043

Cited by 54 publications

(39 citation statements)

References 8 publications

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“…BNYVV is transmitted in the field by the soil-borne fungus Polymyxa betae and is found associated with roots of sugar beet displaying symptoms of rhizomania disease (Tamada & Baba, 1973). Under these conditions all four genome components are invariably present (Koenig et al, 1986;Bouzoubaa et al, 1988;Tamada et al, 1989). Isolates of BNYVV maintained in the laboratory, on the other hand, are customarily propagated by mechanical inoculation onto leaves of Chenopodium quinoa or Tetragonia expansa and in these circumstances neither of the two smallest genomic RNAs, RNA-3 [approximately 1850 nucleotides (nO including the 3' poly(A) tail] and RNA-4 (approximately 1550 nt), is required for infection (Koenig et aL, 1986; t Present address: Max-Planck Institut ffir Biochemie, Martinsried, 8033 Miinchen, Germany.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Kuszala et al, 1986;Burgermeister et al, 1986;Quillet et al, 1989). These observations are consistent with the idea that the two longest RNAs, RNA-1 and -2, code for basic functions required in all hosts while RNA-3 and -4 are functional in the natural infection process, that is, fungus-mediated infection of roots and proliferation within root tissue (Koenig et al, 1986;Lemaire et al, 1988;Tamada & Abe, 1989). Recent findings indicate that RNA-4 is essential for efficient fungal transmission (Tamada & Abe, 1989) while RNA-3 may facilitate viral multiplication and/or movement in roots (Koenig & Burgermeister, 1989;Tamada & Abe, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Shortened forms of beet necrotic yellow vein virus RNA-3 and -4: internal deletions and a subgenomic RNA

Bouzoubaa

Niesbach-Klösgen

Jupin

et al. 1991

Journal of General Virology

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Beet necrotic yellow vein virus RNA-3 and RNA-4, produced as full-length biologically active transcripts in vitro, can undergo spontaneous internal deletions when inoculated onto Chenopodium quinoa leaves along with RNA-1 and -2. The deletion process is specific, giving rise to only a few major species, and can be rapid; deleted forms appear after only one or two passages in leaves. In one of the shortened forms of RNA-4, the deletion precisely eliminated one copy of a 15 nucleotide (nt) direct sequence repeat from the fulllength prototype sequence, suggesting that 'copychoice' switching of the replicase-template complex from one repeat to the other during RNA replication was responsible for the generation of this deletion. The deletion found in a major shortened form of RNA-3, on the other hand, did not occur near sequence repeats but began with GU and ended with AG like a nuclear intron sequence. Thus it is possible that the deleted sequence has been removed by splicing. However, two other deletions that were characterized were not associated with either of these types of sequence feature. An approximately 600 nt 5'-terminally truncated nonencapsidated form of RNA-3 was also detected in infected plant tissue. The evidence suggests that it is a subgenomic RNA derived from RNA-3.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Shortened forms of beet necrotic yellow vein virus RNA-3 and -4: internal deletions and a subgenomic RNA

Bouzoubaa

Niesbach-Klösgen

Jupin

et al. 1991

Journal of General Virology

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“…These alterations in the deletion mutations of smaller RNAs were associated with changes in symptoms expression of BNYVV, molecular differences between the strain of BNYVV or low virus concentration in the isolates (Henry et al, 1986;Koenig et al, 1986). The yield low amount of dsRNA in infected plants is related specifically to the time of infection and incubation temperature (Valverde et al, 1990).…”

Section: Resultsmentioning

confidence: 99%

Detection of Beet Necrotic Yellow Vein Virus by Double Stranded RNA Analysis

Kiliç

Yardımcı²,

Ürgen³

2013

IJB

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Beet necrotic yellow vein virus (BNYVV), the type member of the Benyvirus genus, has a multipartite, positive-sense single-stranded RNA genome, which consists generally of four, or in some isolates five, distinct RNA species. In this study, 108 BNYVV infected soil samples were collected from Isparta province, Turkey. Sugar beet plants cv Kasandra were grown in these soil samples using bait plant techniques and root samples were then analyzed by dsRNA analysis. The RNA was purified by CF-11 cellulose chromatography and gel electrophoresis. In 108 samples tested, dsRNA profiles were observed in 53 samples. No dsRNA bands were observed in negative control used in the analysis.

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“…The absence of one or both of the smaller RNAs of BNYVV has been observed frequently (3,5,20,21,29,30,40). Although naturally occurring variation in length of BNYVV RNAs 3 and 4 has been reported (2,29), the small internal deletions or loss of entire RNA species usually is attributed to multiple passage through C. quinoa (5,20,30). The nature of tissue selected for purification i.e., root vs. leaf tissue, has also been cited as a possible source of variation in smaller BNYVV RNAs (20).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Beet Soilborne Mosaic Virus, a Furo-like Virus Infecting Sugar Beet

et al. 1997

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Beet soilborne mosaic virus (BSBMV) is a rigid rod-shaped virus transmitted by Polymyxa betae. Particles were 19 nm wide and ranged from 50 to over 400 nm, but no consistent modal lengths could be determined. Nucleic acids extracted from virions were polyadenylated and typically separated into three or four discrete bands of variable size by agarose-formaldehyde gel electrophoresis. RNA 1 and 2, the largest of the RNAs, consistently averaged 6.7 and 4.6 kb, respectively. The sizes and number of smaller RNA species were variable. The molecular mass of the capsid protein of BSBMV was estimated to be 22.5 kDa. In Northern blots, probes specific to the 3′ end of individual beet necrotic yellow vein virus (BNYVV) RNAs 1–4 hybridized strongly with the corresponding BNYVV RNA species and weakly with BSBMV RNAs 1, 2, and 4. Probes specific to the 5′ end of BNYVV RNAs 1–4 hybridized with BNYVV but not with BSBMV. No cross-reaction between BNYVV and BSBMV was detected in Western blots. In greenhouse studies, root weights of BSBMV-infected plants were significantly lower than mock-inoculated controls but greater than root weights from plants infected with BNYVV. Results of serological, hybridization, and virulence experiments indicate that BSBMV is distinct from BNYVV. However, host range, capsid size, and the number, size, and polyadenylation of its RNAs indicate that BSBMV more closely resembles BNYVV than it does other members of the genus Furovirus.

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Uniform RNA Patterns of Beet Necrotic Yellow Vein Virus in Sugarbeet Roots, but Not in Leaves from Several Plant Species

Cited by 54 publications

References 8 publications

Shortened forms of beet necrotic yellow vein virus RNA-3 and -4: internal deletions and a subgenomic RNA

Shortened forms of beet necrotic yellow vein virus RNA-3 and -4: internal deletions and a subgenomic RNA

Detection of Beet Necrotic Yellow Vein Virus by Double Stranded RNA Analysis

Characteristics of Beet Soilborne Mosaic Virus, a Furo-like Virus Infecting Sugar Beet

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