Unilateral intrauterine insemination with prostatic fluid-sensitized frozen caudal epididymal sperm in beagle dogs

Hori, Tatsuya; Hagiuda, Kumi; Kawakami, Eiichi; Tsutsui, Toshihiko

doi:10.1016/j.theriogenology.2004.05.027

Cited by 44 publications

(66 citation statements)

References 19 publications

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“…Similar to the study by Hori et al [28], the current study also shows that the addition of prostatic fluid prior to freezing decreases the prevalence of retained cytoplasmic droplets after thawing. In contrast to the study by Hori et al, where prostatic fluid decreased the average prevalence of retained cytoplasmic droplets from 61% to 4%, the current study resulted in a much smaller reduction from 65% to 54%.…”

Section: The Effect Of Prostatic Fluidsupporting

confidence: 90%

“…In contrast, the current study shows that the suspension of epididymal spermatozoa in a mixture of BilEq and prostatic fluid improves their longevity. Similar to the current study, Hori et al [28] showed that exposing epididymal spermatozoa to prostatic fluid before extending and freezing them in an extender similar to BilEq also increased their longevity after thawing.…”

Section: The Effect Of Prostatic Fluidsupporting

confidence: 87%

“…The current study as well as that of Hori et al [28] shows that prostatic fluid improves the motility, longevity and morphology of frozen-thawed dog epididymal spermatozoa. Hori et al also showed that prostatic fluid improves the fertility of frozen-thawed dog epididymal spermatozoa inseminated into the uterus.…”

Section: The Effect Of Prostatic Fluidsupporting

confidence: 84%

“…Assuming, as suggested in an earlier paragraph, that spermatozoa from the sperm-rich fraction of the ejaculate result in better motility after thawing than epididymal spermatozoa, the fact that Hori et al [28] exposed epididymal sperm to pure prostatic fluid may have resembled the situation in the sperm-rich fraction of the ejaculate more closely than in the current study, where the epididymal spermatozoa were exposed to a fluid containing only 20% (v/v) of prostatic fluid. The reason why the addition of prostatic fluid to epididymal spermatozoa prior to freezing improved Prog in the study by Hori et al, but not in the current study may be that the concentration of prostatic fluid in the current study was too low.…”

Section: The Effect Of Prostatic Fluidsupporting

confidence: 54%

“…If, instead of directly extending and freezing epididymal spermatozoa in an extender containing Tris, egg yolk and Equex STM paste, the spermatozoa are temporarily exposed to prostatic fluid before such extension and freezing, Hori et al found that Prog after thawing more than doubles to 32.0 ± 1.4 [28]. This motility seems comparable to that obtained with frozen-thawed ejaculated spermatozoa [21], suggesting that an important, if not the only reason for the poor freezability of dog epididymal sperm may be a lack of exposure to prostatic fluid.…”

Section: The Effect Of Prostatic Fluidmentioning

confidence: 99%

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The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

et al. 2007

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Although dog prostatic fluid decreases the longevity of ejaculated dog spermatozoa, it also increases their rate of motility and their fertility after vaginal insemination, as well as the fertility of epididymal spermatozoa after uterine insemination. These findings indicate a need to further characterize the effects of prostatic fluid on dog spermatozoa. This study was done to determine the effects (P < 0.05) of homologous prostatic fluid added prior to cooling, after thawing, or at both times to epididymal spermatozoa from 21 dogs. The

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Section: The Effect Of Prostatic Fluidsupporting

confidence: 90%

Section: The Effect Of Prostatic Fluidsupporting

confidence: 87%

Section: The Effect Of Prostatic Fluidsupporting

confidence: 84%

Section: The Effect Of Prostatic Fluidsupporting

confidence: 54%

Section: The Effect Of Prostatic Fluidmentioning

confidence: 99%

See 3 more Smart Citations

The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

et al. 2007

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Influence of seminal plasma on fertility of fresh and frozen-thawed stallion epididymal spermatozoa

Heise

Kähn

Volkmann

et al. 2010

Animal Reproduction Science

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Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa

Heise¹,

Thompson²,

Gerber³

2011

Animal Reproduction Science

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Fresh and post-thaw parameters (motility, morphology and viability) of stallion epididymal spermatozoa that have been and have not been exposed to seminal plasma were evaluated, and directly compared to fresh and post-thaw parameters of ejaculated spermatozoa.Six sperm categories of each stallion (n= 4) were evaluated for motility, morphology and viability. These categories were fresh ejaculated spermatozoa (Fr-E), fresh epididymal spermatozoa that had been exposed to seminal plasma (Fr-SP+), fresh epididymal spermatozoa that had never been exposed to seminal plasma (Fr-SP-), frozen-thawed ejaculated spermatozoa (Cr-E), frozen-thawed epididymal spermatozoa that had been exposed to seminal plasma prior to freezing (Cr-SP+) and frozen-thawed epididymal spermatozoa that had never been exposed to seminal plasma (Cr-SP-).Results show that seminal plasma stimulates initial motility of fresh epididymal stallion spermatozoa while this difference in progressive motility is no longer present post-thaw;and that progressive motility of fresh or frozen-thawed ejaculated stallion spermatozoa is not always a good indicator for post-thaw progressive motility of epididymal spermatozoa.This study shows that seminal plasma has a positive influence on the incidence of overall sperm defects, midpiece reflexes and distal cytoplasmic droplets in frozen-thawed stallion epididymal spermatozoa while the occurance of midpiece reflexes is likely to be linked to distal cytoplasmic droplets. Furthermore, seminal plasma does not have an influence on viability of fresh and frozen-thawed morphologically normal epididymal spermatozoa.We recommend the retrograde flushing technique using seminal plasma as flushing medium to harvest and freeze stallion epididymal spermatozoa.

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Unilateral intrauterine insemination with prostatic fluid-sensitized frozen caudal epididymal sperm in beagle dogs

Cited by 44 publications

References 19 publications

The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

The effect of homologous prostatic fluid on motility and morphology of dog epididymal spermatozoa extended and frozen in Biladyl with Equex STM paste or Andromed

Influence of seminal plasma on fertility of fresh and frozen-thawed stallion epididymal spermatozoa

Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa

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