Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish

Wu, Yao Ju; Chen, Yu Lan; Tang, Tso Hao; Ng, Ming Chong; Amstislavskaya, Tamara G.; Tikhonova, Maria A.; Yang, Yi; Lu, Kwok Tung

doi:10.1038/s41598-017-08093-9

Cited by 4 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the putative homologues of mammalian hippocampus and amygdala were defined in the zebrafish Tel region (Ganz et al, 2014). Recent studies has shown LTP of synaptic plasticity occurs in the Tel region of zebrafish brain (Nam et al, 2004;Ng et al, 2012;Wu et al, 2017). Since the Tel is a more prominent region with respect to cognitive functions including learning and memory in zebrafish, age-responsive Smurf2 alterations as reported in the current study imply a role for Smurf2 during aging and age-related cognitive alterations.…”

Section: Discussionsupporting

confidence: 61%

“…Moreover, the putative homologues of mammalian hippocampus and amygdala were already defined in the zebrafish Tel region as the ventral division of the lateral zone of area dorsalis (Dlv in medial pallium) and the medial zone of the dorsal telencephalic area (Dm in ventral pallium), respectively (Ganz et al, 2014). Several studies demonstrated that long-term potentiation (LTP), which is the cellular basis of synaptic plasticity underlying learning and memory, occurs in the Tel region of zebrafish brain (Nam et al, 2004;Ng et al, 2012;Wu et al, 2017). More importantly, although the zebrafish brain has 16 distinct zones of neurogenesis, which is not restricted to the telencephalon, but is widespread throughout the entire brain (Grandel et al, 2006;Kizil et al, 2012), neurogenesis decreases with advancing age in the Tel (Edelmann et al, 2013;Arslan-Ergul et al, 2016).…”

Section: Smurf2 Gene Expression Increases In a Regionspecific Manner During Brain Agingmentioning

confidence: 99%

See 1 more Smart Citation

Expression Levels of SMAD Specific E3 Ubiquitin Protein Ligase 2 (Smurf2) and its Interacting Partners Show Region-specific Alterations During Brain Aging

et al. 2020

View full text Add to dashboard Cite

Aging occurs due to a combination of several factors, such as telomere attrition, cellular senescence, and stem cell exhaustion. The telomere attrition-dependent cellular senescence is regulated by increased levels of SMAD specific E3 ubiquitin protein ligase 2 (smurf2). With age smurf2 expression increases and Smurf2 protein interacts with several regulatory proteins including, Smad7, Ep300, Yy1, Sirt1, Mdm2, and Tp53, likely affecting its function related to cellular aging. The current study aimed at analyzing smurf2 expression in the aged brain because of its potential regulatory roles in the cellular aging process. Zebrafish were used because like humans they age gradually and their genome has 70% similarity. In the current study, we demonstrated that smurf2 gene and protein expression levels altered in a region-specific manner during the aging process. Also, in both young and old brains, Smurf2 protein was enriched in the cytosol. These results imply that during aging Smurf2 is regulated by several mechanisms including post-translational modifications (PTMs) and complex formation. Also, the expression levels of its interacting partners defined by the STRING database, tp53, mdm2, ep300a, yy1a, smad7, and sirt1, were analyzed. Multivariate analysis indicated that smurf2, ep300a, and sirt1, whose proteins regulate ubiquitination, acetylation, and deacetylation of target proteins including Smad7 and Tp53, showed age-and brain region-dependent patterns. Our data suggest a likely balance between Smurf2-and Mdm2mediated ubiquitination, and Ep300a-mediated acetylation/Sirt1-mediated deacetylation, which most possibly affects the functionality of other interacting partners in regulating cellular and synaptic aging and ultimately cognitive dysfunction.

show abstract

Section: Discussionsupporting

confidence: 61%

Section: Smurf2 Gene Expression Increases In a Regionspecific Manner During Brain Agingmentioning

confidence: 99%

Expression Levels of SMAD Specific E3 Ubiquitin Protein Ligase 2 (Smurf2) and its Interacting Partners Show Region-specific Alterations During Brain Aging

et al. 2020

View full text Add to dashboard Cite

show abstract

“…At the synapse level, evidence has already been obtained that zebrafish neuronal plasticity functions similarly to what we know about mammalian hippocampal pyramidal neurons, the well-known place cells. For example, high-frequency electric stimulation of the dorsal telencephalon of the zebrafish induces LTP, whereas low-frequency stimulation induces long-term depression (LTD; Wu et al, 2017 ), synaptic strengthening and weakening processes thought to underly the establishment of medium and long term spatial (relational/configural) memory in mammals (for comprehensive account see Sweatt, 2010 ). Furthermore, just like in mammals, long-term associative memory was found to be dependent upon protein synthesis (Hinz et al, 2013 ).…”

Section: Data On Mechanisms Underlying Elemental and Configural Learnmentioning

confidence: 99%

Elemental and Configural Associative Learning in Spatial Tasks: Could Zebrafish be Used to Advance Our Knowledge?

Buatois

2020

Front. Behav. Neurosci.

View full text Add to dashboard Cite

Spatial learning and memory have been studied for several decades. Analyses of these processes pose fundamental scientific questions but are also relevant from a biomedical perspective. The cellular, synaptic and molecular mechanisms underlying spatial learning have been intensively investigated, yet the behavioral mechanisms/strategies in a spatial task still pose unanswered questions. Spatial learning relies upon configural information about cues in the environment. However, each of these cues can also independently form part of an elemental association with the specific spatial position, and thus spatial tasks may be solved using elemental (single CS and US association) learning. Here, we first briefly review what we know about configural learning from studies with rodents. Subsequently, we discuss the pros and cons of employing a relatively novel laboratory organism, the zebrafish in such studies, providing some examples of methods with which both elemental and configural learning may be explored with this species. Last, we speculate about future research directions focusing on how zebrafish may advance our knowledge. We argue that zebrafish strikes a reasonable compromise between system complexity and practical simplicity and that adding this species to the studies with laboratory rodents will allow us to gain a better understanding of both the evolution of and the mechanisms underlying spatial learning. We conclude that zebrafish research will enhance the translational relevance of our findings.

show abstract

“…Anatomically, it is known that functional equivalents of the mammalian amygdala, striatum and hippocampus are represented in the zebrafish brain by the medial zone of the dorsal telencephalon (Lau et al, 2011), dorsal nucleus of the ventral telencephalon (Lau et al, 2011), and lateral zone of the dorsal telencephalon (Rodríguez-Expósito et al, 2017), respectively. Some cellular neurophysiological processes have been shown to be conserved between mammals and zebrafish: as in rodent hippocampus, high-frequency stimulation-induced long-term potentiation of synaptic strength is N-methyl-D-aspartate (NMDA) receptor-dependent in the zebrafish telencephalon (Nam et al, 2004; Ng et al, 2012; Wu et al, 2017). Consistent with these findings, treatment of adult zebrafish with an NMDA-receptor antagonist prevents acquisition of spatial (Cognato et al, 2012) and emotional (Ng et al, 2012) memories.…”

Section: Introductionmentioning

confidence: 99%

Cholinergic Stimulation of the Adult Zebrafish Brain Induces Phosphorylation of Glycogen Synthase Kinase-3 β and Extracellular Signal-Regulated Kinase in the Telencephalon

Mans

Hinton

Payne

et al. 2019

Front. Mol. Neurosci.

View full text Add to dashboard Cite

The sequencing of the zebrafish genome, the emergence of powerful gene-editing tools, and the development of in vivo imaging techniques have propelled the economical zebrafish into prominence as a biomedical research model. Neurodegenerative disorders with a cholinergic component, such as Alzheimer’s and Parkinson’s diseases, are currently modeled using zebrafish. Still, the utility of zebrafish as a research model will not be fully realized until their neurophysiological properties are thoroughly characterized. In mammals, the coupling of cholinergic receptors to the phosphorylation of glycogen synthase kinase-3 β (GSK3β) and extracellular signal-regulated kinase 1/2 (ERK1/2) is of critical importance to cognitive processes and imparts protection against neuropathogenic events. Similarly, it is known that cholinergic receptors are required for learning and memory in zebrafish and that in vivo activation of cholinergic receptors induces transient changes in evoked synaptic transmission in the telencephalon. However, the intracellular events mediating cholinergic processes in zebrafish have yet to be elucidated. In the current study, an ex vivo drug treatment assay was used to demonstrate that carbachol (CCh)-mediated cholinergic stimulation of the intact adult zebrafish brain induces phosphorylation of GSK3β and ERK1/2 in the zebrafish telencephalon. These findings suggest GSK3β and ERK1/2 may underly cognitive processes in zebrafish.

show abstract

Unilateral stimulation of the lateral division of the dorsal telencephalon induces synaptic plasticity in the bilateral medial division of zebrafish

Cited by 4 publications

References 33 publications

Expression Levels of SMAD Specific E3 Ubiquitin Protein Ligase 2 (Smurf2) and its Interacting Partners Show Region-specific Alterations During Brain Aging

Expression Levels of SMAD Specific E3 Ubiquitin Protein Ligase 2 (Smurf2) and its Interacting Partners Show Region-specific Alterations During Brain Aging

Elemental and Configural Associative Learning in Spatial Tasks: Could Zebrafish be Used to Advance Our Knowledge?

Cholinergic Stimulation of the Adult Zebrafish Brain Induces Phosphorylation of Glycogen Synthase Kinase-3 β and Extracellular Signal-Regulated Kinase in the Telencephalon

Contact Info

Product

Resources

About