Unique action of sodium tanshinone II-A sulfonate (DS-201) on the Ca2+ dependent BKCa activation in mouse cerebral arterial smooth muscle cells

Tan, Xiaoqiu; Yang, Yan; Cheng, Jun; Li, Pengyun; Inoue, Ituro; Zeng, Xiaorong

doi:10.1016/j.ejphar.2011.01.028

Cited by 23 publications

(25 citation statements)

References 19 publications

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“…A typical recording of whole-cell macroscopic BK Ca currents is shown in Figure 1A Figure 1E). These properties of BK Ca are consistent with those previously reported [16,30] .…”

Section: Resultssupporting

confidence: 82%

Propofol increases the Ca2+ sensitivity of BKCa in the cerebral arterial smooth muscle cells of mice

et al. 2011

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Aim: Propofol has the side effect of hypotension especially in the elderly and patients with hypertension. Previous studies suggest propofol-caused hypotension results from activation of large conductance Ca 2+ -sensitive K channels (BKCa). In this study, the effects of propofol on the Ca 2+ sensitivity of BKCa were investigated in mice cerebral arterial smooth muscle cells. Methods: Single smooth muscle cells were prepared from the cerebral arteries of mice. Perforated whole-cell recoding was conducted to investigate the whole-cell BKCa current and spontaneous transient outward K + current (STOC). Inside-out patch confi guration was used to record the single channel current and to study the Ca 2+ -and voltage-dependence of BKCa.

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“…A typical recording of whole-cell macroscopic BK Ca currents is shown in Figure 1A Figure 1E). These properties of BK Ca are consistent with those previously reported [16,30] .…”

Section: Resultssupporting

confidence: 82%

Propofol increases the Ca2+ sensitivity of BKCa in the cerebral arterial smooth muscle cells of mice

et al. 2011

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“…Whole-cell current, STOCs, single-channel current, and macromembrane current of BK Ca channels were recorded in human VSMCs with the techniques as described previously 15,16 and in the online-only Data Supplement Methods.…”

Section: Electrophysiologymentioning

confidence: 99%

Function of BK _Ca Channels Is Reduced in Human Vascular Smooth Muscle Cells From Han Chinese Patients With Hypertension

Yang

Cheng

et al. 2013

Hypertension

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It is well known that chronic hypertension is associated with increased morbidity and mortality from stroke, coronary artery disease, congestive heart failure, renal disease, and so on.1,2 Arterial tone is persistently increased as a result of malfunction of vessel relaxation in chronic hypertension.2 It is well recognized that arterial tone is regulated by functional balance of the ion channels responsible for cellular depolarization and hyperpolarization. The increased arterial tone is mainly related to depolarization of smooth muscle, which may have resulted from dysfunction of ion channels responsible for cell membrane hyperpolarization. 3 The membrane depolarization activates voltage-dependent l-type Ca 2+ channels, induces an increase in Ca 2+ influx and global intracellular Ca 2+ level, and causes vessel constriction. 4,5 It is believed that large-conductance Ca 2+ -activated K + (BK Ca ) channels play an important role in hyperpolarization of vascular smooth muscle cells (VSMCs).6 BK Ca channels are activated by intracellular local Ca 2+ release events through ryanodine receptors (Ca 2+ sparks) from the sarcoplasmic reticulum and subsequently induce a hyperpolarization that opposes vasoconstriction. 6-8The Ca 2+ sparks are highly localized and short-lived Ca 2+ transients, which is a local Ca 2+ signaling to induce spontaneous transient outward currents (STOCs) in VSMCs and neurons, and mediate different physiological functions. 8,9 In VSMCs, functional coupling of sparks to STOCs hyperpolarizes the membrane potential, which in turn closes the voltage-dependent l-type Ca 2+ channels, decreases global [Ca 2+ ] i , and induces vascular relaxation. 7 Studies from animal models demonstrated that a reduced activity of Ca 2+ sparks and STOCs is involved in an increase of vascular tone in hypertension. [10][11][12] However, little information is available in literature regarding BK Ca channel activity in human arterial cells/tissue from patients with hypertension. The present study was therefore to investigate whether BK Ca activity is altered in VSMCs isolated from mesentery arterial tissues of Han Chinese patients with hypertension using approaches of electrophysiology and molecular biology.Abstract-Chronic hypertension is associated with an impaired vascular relaxation caused by an increased vascular tone; however, the underlying mechanisms are not fully understood in human patients. The present study was to investigate whether large-conductance Ca 2+-and voltage-activated K + (BK Ca ) channels are involved in dysfunctional relaxation of artery in Han Chinese patients with hypertension using the perforated patch clamp, inside-out single-channel, and macromembrane patch recording techniques to determine whole-cell current, spontaneous transient outward current, open probability, and Ca 2+ sensitivity and the reverse transcription polymerase chain reaction and Western blot analysis to examine the gene and protein expression of α-subunit (KCa1.1) and β1-subunit (KCNMB1) of BK Ca channels in isolated human ...

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“…The terpenoid tanshinone IIA from Salvia miltiorrhiza, which was reported to exhibit anticonvulsant effects [139], was also shown to enhance BK Ca channels [140,141], KCNQ1/KCNE1 K + channels [142], and hyperpolarization-activated cyclic nucleotide-gated (HCN) channels [143], and to inhibit glutamate release from rat cortical synaptosomes via the inhibition of presynaptic Ca 2+ entry and inhibition of the mitogen-activated protein kinase (MEK) [144]. Ginsenosides have been shown to interact with a variety of ion channels, including the GABA A receptor (Rg3) [145], KCNQ1 K + channels (Rg3) [146], Na + channels (Rg3) [147], and L-type, N-type, P/Q-type, and T-type Ca 2+ channels [148].…”

Section: Other Ion Channelsmentioning

confidence: 98%

A pharmacological basis of herbal medicines for epilepsy

Sucher¹,

Carles²

2015

Epilepsy & Behavior

125

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Unique action of sodium tanshinone II-A sulfonate (DS-201) on the Ca2+ dependent BKCa activation in mouse cerebral arterial smooth muscle cells

Cited by 23 publications

References 19 publications

Propofol increases the Ca2+ sensitivity of BKCa in the cerebral arterial smooth muscle cells of mice

Propofol increases the Ca2+ sensitivity of BKCa in the cerebral arterial smooth muscle cells of mice

Function of BK _Ca Channels Is Reduced in Human Vascular Smooth Muscle Cells From Han Chinese Patients With Hypertension

A pharmacological basis of herbal medicines for epilepsy

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Unique action of sodium tanshinone II-A sulfonate (DS-201) on the Ca2+ dependent BKCa activation in mouse cerebral arterial smooth muscle cells

Cited by 23 publications

References 19 publications

Propofol increases the Ca2+ sensitivity of BKCa in the cerebral arterial smooth muscle cells of mice

Propofol increases the Ca2+ sensitivity of BKCa in the cerebral arterial smooth muscle cells of mice

Function of BK Ca Channels Is Reduced in Human Vascular Smooth Muscle Cells From Han Chinese Patients With Hypertension

A pharmacological basis of herbal medicines for epilepsy

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Function of BK _Ca Channels Is Reduced in Human Vascular Smooth Muscle Cells From Han Chinese Patients With Hypertension