Unique lesions and viral-like particles found in growth retarded black tiger shrimp Penaeus monodon from East Africa

Anantasomboon, Gun; Sriurairatana, Siriporn; Flegel, Timothy W.; Withyachumnarnkul, Boonsirm

doi:10.1016/j.aquaculture.2005.08.021

Cited by 15 publications

(22 citation statements)

References 24 publications

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“…[10]. Subsequently, a similar condition was also reported in P. monodon from East Africa also [2]. Emergence of MSGS started following large-scale importations of Pacific white shrimp (L. vannamei).…”

Section: Monodon Slow Growth Syndrome (Msgs)supporting

confidence: 57%

“…The LO is suggested to be a major site for viral predilection as indicated by formation of LO spheroids as reported in a number of viral diseases of penaeid species [2,3,44,45]. In LSNV-infected P. monodon, viral-like particles could be observed by TEM only in the LO tubules which were ISH positive [55].…”

Section: Predisposing Factorsmentioning

confidence: 79%

“…It was reported that the mean body weight of the shrimp at the sixth month in affected ponds was 19 ± 4 g, which was 30 % less than the expected size of normal shrimp grown within the same period. However, the phenomenon of MSGS occurring in this region and the growth retardation reported in farmed shrimp from East Africa is the same, remains unknown [2].…”

Section: Geographical Distribution Of Msgs and Prevalence Of Lsnvmentioning

confidence: 93%

“…Incidence of MSGS has been reported to be low in farms adopting GMP, GAqP or using SPF PL [56]. It would be prudent to limit the potential spread of MSGS by initiating quarantine safeguards in the movement of living shrimp stocks for aquaculture and to exercise due caution to prevent its spread by live broodstock or PL for aquaculture [2,15]. Further, the National authorities should increase surveillance for slow growth syndrome in P. monodon.…”

Section: Strategies For the Control Of Lsnvmentioning

confidence: 99%

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Laem-Singh Virus: A Probable Etiological Agent Associated with Monodon Slow Growth Syndrome in Farmed Black Tiger Shrimp (Penaeus monodon)

et al. 2012

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Among the emerging diseases in shrimp aquaculture, monodon slow growth syndrome (MSGS) is a major concern in South and Southeast Asia. Shrimp farming in Thailand was severely affected during 2000-2002 due to MSGS, which caused an economic loss, of about US$ 300 million. MSGS is characterized by abnormally slow growth with coefficients of size variation of [35 %, that has impacted P. monodon production in Thailand. A new shrimp virus, Laem-Singh virus (LSNV) was identified to be associated in MSGS affected shrimp. LSNV a RNA virus of about 25 nm diameter is phylogenetically related to the insect-borne viruses in the families Barnaviridae, Tymoviridae and Sobemoviridae an important histopathological observation is exclusively noticed in growth-retarded shrimp. The LSNV infections have been confirmed in various organs of infected shrimp such as lymphoid organ, gills and nervous tissues by various diagnostic techniques such as reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization, quantitative real-time RT-PCR and reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick (RT-LAMP-LFD) and these tools are available for the diagnosis of LSNV. Recently, an integrase containing element has been identified in absolute association with LSNV in stunted growth shrimp. The transmission of LSNV through horizontal and vertical routes has been experimentally demonstrated. The known natural host-range of LSNV includes P. monodon and other penaeid shrimp. The putative RdRp gene involved in replication of LSNV was targeted for dsRNA-mediated gene silencing and appeared to be effective in a dose-dependent manner. Since the discovery of LSNV in 2006 in Thailand, it has been added to the list of viruses to be excluded from domesticated specific pathogenfree stocks of P. monodon and it has been recommended that shrimp farmers avoid stocking post larvae positive for LSNV to prevent MSGS in their farms.

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Section: Monodon Slow Growth Syndrome (Msgs)supporting

confidence: 57%

Section: Predisposing Factorsmentioning

confidence: 79%

Section: Geographical Distribution Of Msgs and Prevalence Of Lsnvmentioning

confidence: 93%

Section: Strategies For the Control Of Lsnvmentioning

confidence: 99%

See 2 more Smart Citations

Laem-Singh Virus: A Probable Etiological Agent Associated with Monodon Slow Growth Syndrome in Farmed Black Tiger Shrimp (Penaeus monodon)

et al. 2012

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“…We confirmed virulence of the stocks by challenging healthy P. monodon juvenile shrimp (approximately 7 -10 g) as previously described (Anantasomboon et al, 2006;Rijiravanich et al, 2008). High mortality resulted, beginning 2 days post challenge, and moribund shrimp were positive for YHV by RT-PCR assay or WSSV by PCR assay using appropriate commercial kits from Farming Intelligene (Taipei, Taiwan).…”

Section: Sources Of Viral Inoculummentioning

confidence: 81%

Persistent Expression of Shrimp-virus Antigens in Two Insect Cell Lines Challenged with Two Shrimp Viruses

et al. 2009

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ABSTRACT-Experiments with crustacean viruses are hampered by lack of susceptible continuous cell lines. To overcome this problem, immortal mosquito and lepidopteran cell lines were both separately challenged with a shrimp DNA virus (white spot syndrome virus: WSSV, = PRDV) and RNA virus (yellow head virus: YHV) followed by serial, split-passage with immunohistochemical monitoring by confocal laser microscopy using labeled monoclonal antibodies to shrimp viral antigens. Stable, immortal cultures with 100% of the cells expressing shrimp-virus antigens were obtained, although the infected cells appeared grossly normal by phase contrast microscopy. Nor did they show any ultrastructural modifications characteristic of the challenge viruses. These persistently-expressing insect cell cultures were stable and could be continuously passaged, stored and revived as required. Since disparate viruses and insect cells were used, this appears to be a generic process that may be applicable to other shrimp viruses as well.

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Viruses Affecting Crustaceans

Bateman

2021

Studies in Viral Ecology

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Unique lesions and viral-like particles found in growth retarded black tiger shrimp Penaeus monodon from East Africa

Cited by 15 publications

References 24 publications

Laem-Singh Virus: A Probable Etiological Agent Associated with Monodon Slow Growth Syndrome in Farmed Black Tiger Shrimp (Penaeus monodon)

Laem-Singh Virus: A Probable Etiological Agent Associated with Monodon Slow Growth Syndrome in Farmed Black Tiger Shrimp (Penaeus monodon)

Persistent Expression of Shrimp-virus Antigens in Two Insect Cell Lines Challenged with Two Shrimp Viruses

Viruses Affecting Crustaceans

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