Unique opportunities for future research on the alternative oxidase of plants

McDonald, Allison E.

doi:10.1093/plphys/kiac555

Cited by 7 publications

(3 citation statements)

References 47 publications

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“…When a yeast strain is rendered respiration deficient by a mutation, growth is supported on fermentable carbon sources, such as glucose or galactose [8]. As in humans, Saccharomyces cerevisiae relies solely on COX for oxidative respiration, lacking the alternative ubiquinol oxidase that is found in some species of yeasts and other eukaryotic organisms [9,10]. Furthermore, S. cerevisiae is a well-suited model due to the high degree of similarity between the COX assembly processes in mammalian and yeast cells [11].…”

Section: Yeast As a Model System For Human Cell Biologymentioning

confidence: 99%

More than Just Bread and Wine: Using Yeast to Understand Inherited Cytochrome Oxidase Deficiencies in Humans

Caron-Godon,

Collington,

Wolf

et al. 2024

IJMS

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Inherited defects in cytochrome c oxidase (COX) are associated with a substantial subset of diseases adversely affecting the structure and function of the mitochondrial respiratory chain. This multi-subunit enzyme consists of 14 subunits and numerous cofactors, and it requires the function of some 30 proteins to assemble. COX assembly was first shown to be the primary defect in the majority of COX deficiencies 36 years ago. Over the last three decades, most COX assembly genes have been identified in the yeast Saccharomyces cerevisiae, and studies in yeast have proven instrumental in testing the impact of mutations identified in patients with a specific COX deficiency. The advent of accessible genome-wide sequencing capabilities has led to more patient mutations being identified, with the subsequent identification of several new COX assembly factors. However, the lack of genotype–phenotype correlations and the large number of genes involved in generating a functional COX mean that functional studies must be undertaken to assign a genetic variant as being causal. In this review, we provide a brief overview of the use of yeast as a model system and briefly compare the COX assembly process in yeast and humans. We focus primarily on the studies in yeast that have allowed us to both identify new COX assembly factors and to demonstrate the pathogenicity of a subset of the mutations that have been identified in patients with inherited defects in COX. We conclude with an overview of the areas in which studies in yeast are likely to continue to contribute to progress in understanding disease arising from inherited COX deficiencies.

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Section: Yeast As a Model System For Human Cell Biologymentioning

confidence: 99%

More than Just Bread and Wine: Using Yeast to Understand Inherited Cytochrome Oxidase Deficiencies in Humans

Caron-Godon,

Collington,

Wolf

et al. 2024

IJMS

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“…There are two pathways involved in respiration: the cytochrome pathway, which is found in nearly all organisms, and the alternate respiration pathway. The alternate respiration pathway is activated when the cytochrome pathway experiences stress conditions like low temperature, drought, ozone, and high salinity soils [31]. The impact of CO 2 on mitochondrial respiration is of great significance in the investigation of plant respiratory metabolism.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Proteomic Responses of the Tetraploid Robinia pseudoacacia L. to High CO2 Levels

Li,

Zhang,

Lei

et al. 2024

IJMS

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The increase in atmospheric CO2 concentration is a significant factor in triggering global warming. CO2 is essential for plant photosynthesis, but excessive CO2 can negatively impact photosynthesis and its associated physiological and biochemical processes. The tetraploid Robinia pseudoacacia L., a superior and improved variety, exhibits high tolerance to abiotic stress. In this study, we investigated the physiological and proteomic response mechanisms of the tetraploid R. pseudoacacia under high CO2 treatment. The results of our physiological and biochemical analyses revealed that a 5% high concentration of CO2 hindered the growth and development of the tetraploid R. pseudoacacia and caused severe damage to the leaves. Additionally, it significantly reduced photosynthetic parameters such as Pn, Gs, Tr, and Ci, as well as respiration. The levels of chlorophyll (Chl a and b) and the fluorescent parameters of chlorophyll (Fm, Fv/Fm, qP, and ETR) also significantly decreased. Conversely, the levels of ROS (H2O2 and O2·−) were significantly increased, while the activities of antioxidant enzymes (SOD, CAT, GR, and APX) were significantly decreased. Furthermore, high CO2 induced stomatal closure by promoting the accumulation of ROS and NO in guard cells. Through a proteomic analysis, we identified a total of 1652 DAPs after high CO2 treatment. GO functional annotation revealed that these DAPs were mainly associated with redox activity, catalytic activity, and ion binding. KEGG analysis showed an enrichment of DAPs in metabolic pathways, secondary metabolite biosynthesis, amino acid biosynthesis, and photosynthetic pathways. Overall, our study provides valuable insights into the adaptation mechanisms of the tetraploid R. pseudoacacia to high CO2.

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“…At the level of the whole respiratory chain, Ghifari et al (2023) discuss advancements in understanding the makeup, biogenesis, and turnover of plant oxidative phosphorylation complexes and mechanisms that regulate their biogenesis and activity. At the individual enzyme level, McDonald (2023 ) covers advancements in defining the distribution and physiological functions of the alternative oxidase of the plant mitochondrial electron transport chain and anticipates the progress that genome editing tools and new oxygen sensing technologies now make possible. Finally, at the metabolite level, Le and Millar (2023 ) cover the diverse respiratory substrates that mitochondria import and use in normal and stress conditions, highlighting evidence for metabolic channeling in supply of substrates to respiration.…”

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confidence: 99%