Unique Pakistani gut microbiota highlights population-specific microbiota signatures of type 2 diabetes mellitus

Abstract

Biogeographic variations in the gut microbiota reflect host and environmental factors delineating human populations, and are pivotal to understand global patterns of host-microbiota interactions in health and prevalent lifestyle-related diseases, such as type 2 diabetes mellitus (T2D). Pakistani adults, having an exceptionally high prevalence of T2D, are one of the most understudied populations in microbiota research to date. The aim of the present study is to examine the gut microbiota across individuals from Pakistan and other populations of non-industrialized and industrialized lifestyles with a focus on T2D. The fecal samples from 94 urban-dwelling Pakistani adults with and without T2D were profiled by 16S ribosomal RNA gene amplicon sequencing and qPCR, and plasma samples quantified for circulating levels of lipopolysaccharide binding protein (LBP) and the activation ability of Toll-like receptor (TLR)-signaling. Publicly available datasets generated with comparable molecular methods were retrieved for comparative analysis. Overall, urbanized Pakistanis’ gut microbiota was similar to that of transitional or non-industrialized populations, depleted in Akkermansiaceae and enriched in Prevotellaceae (dominated by the non-Westernized clades of Prevotella copri). The relatively high proportion of Atopobiaceae appeared to be a unique characteristic of the Pakistani gut microbiota. The Pakistanis with T2D had elevated levels of LBP and TLR-signaling in circulation as well as gut microbial signatures atypical of other populations e.g., increased relative abundance of Libanicoccus/Parolsenella, limiting the inter-population extrapolation of gut microbiota-based classifiers for T2D. Taken together, our findings call for more global representation of understudied populations to extend the applicability of microbiota-based diagnostics and therapeutics.

Effect of metformin and metformin/linagliptin on gut microbiota in patients with prediabetes

“…4 B). The effect of M at 12 months suggests cumulative impact, restructuring GM composition and favoring increased SCFA-producing genera abundance 22 .

Figure 3 Schematic diagram of the proposed procedure for the data's clinical and GM analysis.

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Gut microbiota in subjects with prediabetes has a low mediating effect on the hypoglycemic outcome of linagliptin/metformin and metformin.

“…4B). The effect of M at 12 months suggests cumulative impact, restructuring GM composition and favoring increased SCFA-producing genera abundance 22 .…”