Unique Regulation of Immediate Early Gene and Tyrosine Hydroxylase Expression in the Odor-deprived Mouse Olfactory Bulb

Liu, Nian; Cigola, Elena; Tinti, Cristina; Jin, Byung Kwan; Conti, Bruno; Volpe, Bruce T.; Baker, Harriet

doi:10.1074/jbc.274.5.3042

Cited by 47 publications

(55 citation statements)

References 55 publications

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“…For instance, the transcription of the tyrosine hydroxylase gene occurs in SVZ newly generated cells before they attain their final periglomerular position (Baker et al, 2001;Saino-Saito et al, 2004). Interestingly, it has been shown that CREB participates in the Cre DNA-protein complex of the tyrosine hydroxylase promoter in the mouse OB (Liu et al, 1999). These results are consistent with a role for CREB in regulating the transcription of genes involved in the differentiation of SVZ neuroblasts.…”

supporting

confidence: 78%

“…In the OB, several studies indicated an activity-dependent modulation of CREB phosphorylation in different cell types. Indeed, CREB is activated in mitral cells by odor-preference learning (McLean et al, 1999), and a trend to an increase in CREB phosporylation in external OB layers has been observed after unilateral naris closure (Liu et al, 1999). In this study, we demonstrate that CREB phosphorylation is upregulated in rostrally migrating neuroblasts when they reach the OB but sharply decreases after olfactory peripheral afferent denervation (Fig.…”

Section: Discussionsupporting

confidence: 49%

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cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb

Giachino¹,

Marchis²,

Giampietro³

et al. 2005

J. Neurosci.

142

133

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The transcription factor cAMP response element-binding protein (CREB) is involved in multiple aspects of neuronal development and plasticity. Here, we demonstrate that CREB regulates specific phases of adult neurogenesis in the subventricular zone/olfactory bulb (SVZ/OB) system. Combining immunohistochemistry with bromodeoxyuridine treatments, cell tracer injections, cell transplants, and quantitative analyses, we show that although CREB is expressed by the SVZ neuroblasts throughout the neurogenic process, its phosphorylation is transient and parallels neuronal differentiation, increasing during the late phase of tangential migration and decreasing after dendrite elongation and spine formation. In vitro, inhibition of CREB function impairs morphological differentiation of SVZderived neuroblasts. Transgenic mice lacking CREB, in a null CREM genetic background, show reduced survival of newborn neurons in the OB. This finding is further supported by peripheral afferent denervation experiments resulting in downregulation of CREB phosphorylation in neuroblasts, the survival of which appears heavily impaired. Together, these findings provide evidence that CREB regulates differentiation and survival of newborn neurons in the OB.

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supporting

confidence: 78%

Section: Discussionsupporting

confidence: 49%

cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb

Giachino¹,

Marchis²,

Giampietro³

et al. 2005

J. Neurosci.

142

133

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“…Neurotransmission between pre-and postsynaptic fibers in the GL of the OB is extensively modulated by periglomerular interneurons that exclusively express tyrosine TH, CB, or calretinin (CR) (19). Deprivation of presynaptic input from the ONL in rodents, either by treatment with zinc sulfate or by naris occlusion, was previously shown to reduce both TH expression and dopamine levels in the GL of the OB (20,21), whereas naris reopening resulted in the generation of new TH + interneurons (22). We therefore expected that the reduced thickness of the ONL in the OB of Gnao −/− mice would be associated with a reduction in TH expression or a reduced number of TH + periglomerular cells.…”

Section: Resultsmentioning

confidence: 99%

Development of the main olfactory system and main olfactory epithelium-dependent male mating behavior are altered in G _o -deficient mice

Choi

Kim

Choi

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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In mammals, initial detection of olfactory stimuli is mediated by sensory neurons in the main olfactory epithelium (MOE) and the vomeronasal organ (VNO). The heterotrimeric GTP-binding protein G o is widely expressed in the MOE and VNO of mice. Early studies indicated that G o expression in VNO sensory neurons is critical for directing social and sexual behaviors in female mice [Oboti L, et al. (2014) BMC Biol 12:31]. However, the physiological functions of G o in the MOE have remained poorly defined. Here, we examined the role of G o in the MOE using mice lacking the α subunit of G o . Development of the olfactory bulb (OB) was perturbed in mutant mice as a result of reduced neurogenesis and increased cell death. The balance between cell types of OB interneurons was altered in mutant mice, with an increase in the number of tyrosine hydroxylase-positive interneurons at the expense of calbindin-positive interneurons. Sexual behavior toward female mice and preference for female urine odors by olfactory sensory neurons in the MOE were abolished in mutant male mice. Our data suggest that G o signaling is essential for the structural and functional integrity of the MOE and for specification of OB interneurons, which in turn are required for the transmission of pheromone signals and the initiation of mating behavior with the opposite sex.heterotrimeric G o protein | olfactory mucosa | olfactory bulb interneuron | tyrosine monooxygenase | sexual behavior P heromones evoke diverse social and sexual behaviors in animals of the same species. In mammals, initial detection of olfactory stimuli is mediated by sensory neurons in the main olfactory epithelium (MOE) and vomeronasal organ (VNO). Early studies indicated that the VNO is largely responsible for the detection of pheromones and the consequent direction of social and sexual behaviors (1). VNO sensory neurons (VSNs) express distinct types of vomeronasal receptors: type 1 (V1Rs) in the apical region and type 2 (V2Rs) in the basal region. These receptors couple, respectively, to G i and G o members of the heterotrimeric GTPbinding protein family. It has been suggested that, following ligand binding to V1Rs and V2Rs, the Gβγ subunits released from G i and G o activate phospholipase C and increase the intracellular concentration of Ca 2+ . Ca 2+ ions induce opening of small Ca 2+ -activated K + (SK3) channels, subsequently activating Ca 2+ -activated Cl − channels (CACCs). In the VNO, Gβγ can also directly induce the opening of G protein-activated inward rectifying K + (GIRK) channels independently of transient receptor potential cation channel C2 (TRPC2) (2). Because of the VNO's high luminal K + concentration (2), opening of SK3 and GIRK channels leads to depolarization of VSNs and transmission of pheromone signals. Targeted deletion of the TRPC2 gene abrogates gender identification and intermale aggression without affecting male-to-female mating behavior in male mice (3, 4). Targeted deletion of the GIRK1 gene partially attenuates mating behavior of the male toward fem...

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“…In the olfactory system, alterations of peripheral sensory input, either by deafferentation (Margolis et al, 1974;Baker et al, 1988;Ferraris et al, 1997; Casabona et al, 1998; Cummings et al, 2000) as well as nares closure (Brunjes et al, 1985;Cho et al, 1996;Jin et al, 1996;Liu et al, 1999;Hamilton and Coppola, 2003), alters gene expression in the olfactory bulb. However, reductions in sensory input may have wide effects by reducing activity not only in the olfactory bulb but also in higher-order olfactory areas such as the piriform cortex.…”

Section: Activity-dependent Transsynaptic Plasticitymentioning

confidence: 99%

“…Peripheral deafferentation and reduced odor-evoked activity causes dramatic morphologic and biochemical changes in the OB (Margolis et al, 1974;Jin et al, 1996;Ferraris et al, 1997;Casabona et al, 1998;Liu et al, 1999;Fiske and Brunjes, 2001;Hamilton and Coppola, 2003), alteration in synaptic physiology (Brunjes, 1994;Wilson, 1995;Philpot et al, 1997;Franks and Isaacson, 2005), as well as significant changes in neurogenesis of olfactory sensory neurons (Farbman et al, 1988;Cummings et al, 2000). Bulbectomy, which removes the first synapse of the olfactory system and the neurons whose axons project to the PC, results in argyrophilic degenerative changes and cell death of pyramidal neurons in adult rat piriform cortex ipsilateral to the lesion (Heimer and Kalil, 1978;Capurso et al, 1997;Leung and Wilson, 2003).…”

Section: Introductionmentioning

confidence: 99%

Odorant Deprivation Reversibly Modulates Transsynaptic Changes in the NR2B-Mediated CREB Pathway in Mouse Piriform Cortex

Kim¹,

Puché²,

Margolis³

2006

J. Neurosci.

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The olfactory system is an outstanding model for understanding activity-dependent neuronal plasticity in mammals. Olfactory sensory neurons (OSNs) in the periphery project onto mitral/tufted cells in the olfactory bulb (OB) and these mitral/tufted cells in turn project to piriform cortex (PC). Numerous studies have examined changes in OB after a permanent OSN ablation, but little is known about "trans-transsynaptic" changes taking place in the PC. Permanent zinc sulfate lesion of the olfactory epithelium resulted in a selective loss of the NMDA receptor NR2B protein and mRNA expression in pyramidal cells in layer IIb of PC after 2-7 d. Regulatory elements affected by NR2B signaling, namely the phosphorylation of CREB, were also downregulated only in layer IIb neurons. These changes could be caused by OSN axon loss in the zinc sulfate lesion, or to a reduced activity. To test this hypothesis, we performed both permanent and reversible naris occlusion, which blocks odorant access to the nasal cavities and OSN activity. The expression of NR2B and phospho-CREB were downregulated 5 d after occlusion and this reduction was fully restored 10 d after reopening of the naris. Subsequently, we identified the subset of pyramidal cells in layer IIb that are especially sensitive to the loss of odor-evoked activity using double retrograde tracers. In summary, the present study provides an initial characterization of the molecular mechanisms associated with odor stimulation on second order neuronal plasticity and phenotype in the olfactory system.

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Unique Regulation of Immediate Early Gene and Tyrosine Hydroxylase Expression in the Odor-deprived Mouse Olfactory Bulb

Cited by 47 publications

References 55 publications

cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb

cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb

Development of the main olfactory system and main olfactory epithelium-dependent male mating behavior are altered in G _o -deficient mice

Odorant Deprivation Reversibly Modulates Transsynaptic Changes in the NR2B-Mediated CREB Pathway in Mouse Piriform Cortex

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Unique Regulation of Immediate Early Gene and Tyrosine Hydroxylase Expression in the Odor-deprived Mouse Olfactory Bulb

Cited by 47 publications

References 55 publications

cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb

cAMP Response Element-Binding Protein Regulates Differentiation and Survival of Newborn Neurons in the Olfactory Bulb

Development of the main olfactory system and main olfactory epithelium-dependent male mating behavior are altered in G o -deficient mice

Odorant Deprivation Reversibly Modulates Transsynaptic Changes in the NR2B-Mediated CREB Pathway in Mouse Piriform Cortex

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Development of the main olfactory system and main olfactory epithelium-dependent male mating behavior are altered in G _o -deficient mice