Unraveling proteome changes of Holstein beef M. semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry

Yu, Qunli; Wu, Wei; Tian, Xiaojing; Hou, Man; Dai, Ruitong; Li, Xingmin

doi:10.1016/j.jprot.2016.12.012

Cited by 80 publications

(48 citation statements)

References 38 publications

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“…Although the low-temperature autolysis of V. volvacea has been studied extensively, the specific molecular mechanism is still unclear 13 . The observed changes in quality characteristics, such as texture and color, might be caused by the complex biochemical and physiological changes in these compounds during storage 14 . Based on these reasons, selecting an appropriate method is important for studying the autolysis of V. volvacea .…”

Section: Introductionmentioning

confidence: 99%

Differential proteomics study of postharvest Volvariella volvacea during storage at 4 °C

Chen

et al. 2020

Sci Rep

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The postharvest storage of Volvariella volvacea is an important factor limiting the industry development. Low-temperature storage is the traditional storage method used for most edible fungi, but V. volvacea undergoes autolysis at low temperature. To understand the molecular mechanism underlying the low-temperature autolysis of V. volvacea after harvesting, fruiting bodies of V. volvacea strain V23 were stored at 4 °C. Based on our previous study, in which the changes of morphological and physiological indexes during storage for 0, 6, 12, 24, 30, 36, 48 and 60 h were measured; four time points, namely, 0, 12, 24 and 60 h, were selected for this differential proteomics study. The proteomic changes in the postharvest storage samples were studied by isobaric tags for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS). A total of 2,063 proteins were identified, and 192 differentially expressed proteins (DEPs), including 24 up-regulated proteins and 168 down-regulated proteins, were detected after 12 h of storage. After 24 h of storage, 234 DEPs, including 48 up-regulated and 186 down-regulated proteins, were observed, and after 60 h, 415 DEPs, including 65 up-regulated proteins and 350 down-regulated proteins, were observed. An in-depth data analysis showed that the DEPs participated in various cellular processes, particularly metabolic processes. In this study, we combined Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses, and the results focused on oxidative phosphorylation and ubiquitin mediated proteolysis pathways. In addition, sdh2, uba1 and ubc1 was confirmed by quantitative real-time polymerase chain reaction, and the results showed that the expression of these genes were consistent with their protein level. Based on the literature and our results, it is speculated that the identified DEPs, such as ATP1, SDH2, COR1, UBA1, COX4, UBC1 and SKP1 play a key role in the low-temperature autolysis of V. volvacea. Volvariella volvacea is a commercially important edible fungus cultivated in tropical and subtropical regions, particularly in Southeast Asian countries 1,2. In recent years, the market demand for V. volvacea has rapidly increased due to its unique flavor 3 and high nutritional value 4. V. volvacea also has pharmaceutical value because it contains antitumor polysaccharides and immunomodulatory lectins 5-7. V. volvacea is delicious and mainly sold fresh. In addition, this fungus exhibits rapid growth and development, has high respiratory intensity, and is easy-to-open umbrella at 28-35 °C, and these features are responsible for the loss of its edible value over a short period of time 8. In addition, V. volvacea is native to tropical and subtropical regions and is thus sensitive to low temperature. The fungus is usually autolysed at 0-5 °C 9,10 , and this autolysis accelerates its decay and causes softening and liquefaction. Therefore, the postharvest storage problem of V. volvacea has become a bottleneck that restr...

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Section: Introductionmentioning

confidence: 99%

Differential proteomics study of postharvest Volvariella volvacea during storage at 4 °C

Chen

et al. 2020

Sci Rep

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“…The use of proteomic tools (two-dimensional electrophoresis and mass spectrometry) to study the muscle-specificity in beef color stability is recent and was first applied by (Joseph, Suman, Rentfrow, Li, & Beach, 2012). Later on, several studies on cattle meat were reported using high-throughput technologies, such as Dot-Blot Gagaoua, Couvreur, Le Bec, Aminot, & Picard, 2017) and label free mass spectrometry strategies (Yu et al, 2017) to identify proteins that would be linked with meat color parameters. These studies highlighted that several biological pathways contribute to the conversion of muscle into meat processes including meat color development.…”

Section: Introductionmentioning

confidence: 99%

Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed

et al. 2018

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Reverse Phase Protein Arrays (RPPA) were applied for the quantification and validation of protein biomarkers of beef qualities on M. longissimus thoracis sampled early post-mortem from young Charolais bulls. pHu was related to six proteins, three of which are glycolytic enzymes (ENO1, ENO3 and TPI1), while others belong to structural (TTN and α-actin) and proteolytic (μ-calpain) pathways. For color traits, several correlations were found, interestingly with structural proteins. The relationships were in some cases trait-dependent. To understand the mechanisms and explore animal variability, color data were categorized into three classes. α-actin and TTN allowed efficient separation of the classes and were strongly related with all color traits. Biomarkers belonging to heat stress and metabolism pathways were also involved. Two identified proteins, namely Four and a half LIM domains 1 (FHL1) and Tripartite motif-containing 72 (TRIM72), were for the first time related to beef color. Overall, these relationships could be used to develop muscle-specific processing strategies to improve beef color stability.

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“…e expression of CD206, TGF-β, and IL-10 is highly expressed in M2 macrophages [33,34]. IL-4 can promote the expression of CD206 in macrophages [35,36]. We found that TCM treatment promoted the expression of TNF-α and IL-6 in IL-4-induced RAW264.7 cells, while TCM treatment downregulated the expression of IL-10 and TGF-β in IL-4-induced RAW264.7 cells.…”

Section: Discussionmentioning

confidence: 65%

Mechanism of Xu Li’s Experiential Prescription for the Treatment of EGFR‐Positive NSCLC

Zhang

Wang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The non-small-cell lung cancer (NSCLC) is the most common lung cancer which seriously threatens the human health. Xu Li’s experiential prescription (XLEP) can treat the NSCLC. However, whether XLEP can regulate the autophagy in the EGFR-positive NSCLC still remains unknown. We found that the cellular activity of drug-resistant cells and sensitive cells were all decreased in the TCM group and TCM + Gef group. The expression of autophagy-associated proteins (mTOR and Beclin1-Vps34) in drug-resistant cells was decreased in the TCM group, while the expression of autophagy-associated proteins in sensitive cells was all decreased in the TCM + Gef group. The ratio of M1/M2 macrophages was increased when IL-4-induced RAW264.7 was treated with TCM. TCM treatment promoted the expression of CCL2 and CCL3 while it downregulated the CCL22 level among A549, H1975, and PC9 cells. The expression of TNF-α and IL-6 was increased, and the expression of IL-10 and TGF-β was decreased in IL-4-induced RAW264.7 cells treated with TCM. And, TCM treatment also decreased the expression of Fizz1 and TGM2. In conclusion, this study indicated that XLEP could suppress the proliferation of EGFR-TKI-resistant cancer cells and increase the ratio of M1/M2 macrophages by inhibiting autophagy to treat the drug-resistant EGFR-positive NSCLC.

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Unraveling proteome changes of Holstein beef M. semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry

Cited by 80 publications

References 38 publications

Differential proteomics study of postharvest Volvariella volvacea during storage at 4 °C

Differential proteomics study of postharvest Volvariella volvacea during storage at 4 °C

Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed

Mechanism of Xu Li’s Experiential Prescription for the Treatment of EGFR‐Positive NSCLC

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