Unravelling HP1 functions: post-transcriptional regulation of stem cell fate

Casale, Assunta Maria; Cappucci, Ugo; Piacentini, Lucia

doi:10.1007/s00412-021-00760-1

Cited by 7 publications

(4 citation statements)

References 113 publications

(141 reference statements)

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“…To further investigate the hypothesis and examine the putative GSC cluster, we plotted the GSC-related gene expression in pseudotime. Similar expression profiles of functional GSC differentiation genes (bam, bgcn, out, twin and Set1) (43)(44)(45)(46) and GSC maintenance genes (aret, Mei-P26, egg, Myc and Hrb98DE) (47)(48)(49)(50)(51) were showed in trajectory (Fig. 2D).…”

Section: Identification Of Gsc Cluster and 2 Distinct Undifferentiate...mentioning

confidence: 54%

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

Sun

Nystul

Zhong

2022

Preprint

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Drosophila ovarian germline stem cells (GSCs) are powerful model for stem cell research. However, due to the scarcity of GSCs in ovarian tissue, it is difficult to obtain the transcriptional profile of GSCs and identify novel GSC markers. In this study, we took advantage of single cell RNA sequencing (scRNA-seq) to profile the germline cells and somatic cells in wild type Drosophila ovary. We then performed an in vivo RNAi screen and network analysis to identify genes that are involved in the early stages of germ cell differentiation. We identified 33 genes with limited expression during early germ cell development and identified 19 genes that potentially regulate germ cell differentiation. Among these, an uncharacterized gene, which we named eggplant (eggpl), is highly expressed in GSC and downregulated in early daughter cells. Upon RNAi knockdown of eggpl, we observed an increase in germ cell proliferation, an accumulation of cysts in the early mitotic (2- and 4-cell) stages and an increase in overall ovary size compared to control when flies were maintained on a standard diet. In flies fed a rich yeast diet, the expression of eggpl was significantly lower and the effects of eggpl RNAi were suppressed, suggesting that downregulation of eggpl may link nutritional status to germ cell proliferation and differentiation. We also found that the matrix metalloproteases, Mmp1 and Mmp2 as well as the tissue inhibitor of metalloproteases (Timp) are additional regulators of eggpl. Collectively, this study provides new insight into the signaling networks that regulate early germ cell development and identifies eggpl as a key player in this process.

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Section: Identification Of Gsc Cluster and 2 Distinct Undifferentiate...mentioning

confidence: 54%

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

Sun

Nystul

Zhong

2022

Preprint

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“…Large-scale mapping of in vivo binding sites of chromatin proteins using tethered dam methyltransferase [96,97] showed that the clustered Bari1 elements in the h39 region of the 2nd chromosome are targets of HP1, a chromatin protein mainly involved in heterochromatin formation [98]. It has been reported that Bari1 also contains Polycomb responsive elements that could collaborate in recruiting additional heterochromatin components [91].…”

Section: Possible Role In Chromatin Assemblymentioning

confidence: 99%

What Have We Learned in 30 Years of Investigations on Bari Transposons?

Palazzo

Caizzi

Moschetti

et al. 2022

Cells

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Transposable elements (TEs) have been historically depicted as detrimental genetic entities that selfishly aim at perpetuating themselves, invading genomes, and destroying genes. Scientists often co-opt “special” TEs to develop new and powerful genetic tools, that will hopefully aid in changing the future of the human being. However, many TEs are gentle, rarely unleash themselves to harm the genome, and bashfully contribute to generating diversity and novelty in the genomes they have colonized, yet they offer the opportunity to develop new molecular tools. In this review we summarize 30 years of research focused on the Bari transposons. Bari is a “normal” transposon family that has colonized the genomes of several Drosophila species and introduced genomic novelties in the melanogaster species. We discuss how these results have contributed to advance the field of TE research and what future studies can still add to the current knowledge.

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“…To further investigate this hypothesis and examine the putative GSC cluster, we plotted the GSC‐related gene expression in pseudotime. Similar expression profiles of functional GSC differentiation genes ( bam , bgcn , out , twin , and Set1 ; Steinhauer & Kalfayan, 1992; Ohlstein et al , 2000; Fu et al , 2015; Vidaurre & Chen, 2021) and GSC maintenance genes ( aret , Mei‐P26 , egg , Myc , and Hrb98DE ; Rhiner et al , 2009; Ji & Tulin, 2012; Blatt et al , 2020; Rastegari et al , 2020; Casale et al , 2021) were revealed in the trajectory (Fig 2E). Consistent with our expectations, we observed low expression of genes associated with differentiation and high expression of genes associated with self‐renewal, while their expression patterns along the trajectory were gradually changed over time.…”

Section: Resultsmentioning

confidence: 99%

Single‐cell RNA sequencing identifies eggplant as a regulator of germ cell development in Drosophila

Sun,

Nystul,

Zhong

2023

EMBO Reports

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Drosophila ovarian germline stem cells (GSCs) are a powerful model for stem cell research. In this study, we use single‐cell RNA sequencing (scRNA‐seq), an RNAi screen and bioinformatic analysis, to identify genes involved in germ cell differentiation, including 34 genes with upregulated expression during early germ cell development and 19 genes that may regulate germ cell differentiation. Among these, a gene we have named eggplant (eggpl) is highly expressed in GSCs and downregulated in early daughter cells. RNAi knockdown of eggpl causes germ cell proliferation and differentiation defects. In flies fed a rich yeast diet, the expression of eggpl is significantly lower and knockdown or knockout of eggpl phenocopies a rich diet. In addition, eggpl knockdown suppresses the reduction in germ cell proliferation caused by inhibition of the insulin effector PI3K. These findings suggest that downregulation of eggpl links nutritional status to germ cell proliferation and differentiation. Collectively, this study provides new insights into the signaling networks that regulate early germ cell development and identifies eggpl as a key player in this process.

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Unravelling HP1 functions: post-transcriptional regulation of stem cell fate

Cited by 7 publications

References 113 publications

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

What Have We Learned in 30 Years of Investigations on Bari Transposons?

Single‐cell RNA sequencing identifies eggplant as a regulator of germ cell development in Drosophila

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