Unravelling the Biological Activities of the Byttneria pilosa Leaves Using Experimental and Computational Approaches

Abstract

Byttneria pilosa is locally known as Harijora, and used by the native hill-tract people of Bangladesh for the treatment of rheumatalgia, snake bite, syphilis, fractured bones, elephantiasis and an antidote for poisoning. The present study was carried out to determine the possible anti-inflammatory, analgesic, neuropharmacological and anti-diarrhoeal activity of the methanol extract of B. pilosa leaves (MEBPL) through in vitro, in vivo and in silico approaches. In the anti-inflammatory study, evaluated by membrane stabilizing and protein denaturation methods, MEBPL showed a significant and dose dependent inhibition. The analgesic effect of MEBPL tested by inducing acetic acid and formalin revealed significant inhibition of pain in both tests. During the anxiolytic evaluation, the extract exhibited a significant and dose-dependent reduction of anxiety-like behaviour in mice. Similarly, mice treated with MEBPL demonstrated dose-dependent reduction in locomotion effect in the open field test and increased sedative effect in the thiopental sodium induced sleeping test. MEBPL also showed good anti-diarrheal activity in both castor oil induced diarrheal and intestinal motility tests. Besides, a previously isolated compound (beta-sitosterol) exhibited good binding affinity in docking and drug-likeliness properties in ADME/T studies. Overall, B. pilosa is a biologically active plant and could be a potential source of drug leads, which warrants further advanced study.

Antioxidant-Rich Woodfordia fruticosa Leaf Extract Alleviates Depressive-Like Behaviors and Impede Hyperglycemia

“…In addition to pharmacokinetic profiles, all compounds satisfied most of the absorption (excluding GI absorption) and metabolism parameters. They were also evaluated as non-substrates of P-gp., P-gp tends to have a greater effect on preventing the cellular absorption of drugs from the intestinal lumen to epithelial cells [ 160 ]. Therefore, the oral availability of the compounds tested would not be impaired by P-gp.…”

Scrutinizing pharmacological efficiency for Acacia auriculiformis by experimental and computational approach

“…In the living body, HRBC lysis (human red blood cell) and protein denaturation are often caused by inflammation, which initiates by the excess synthesis of COX [24]. During these conditions, proteins secondary and tertiary structures gone misplaced due to heat, strong acid or base, injurious agent, stress or organic solvent [10,39]. In the current study, at both of protein denaturation model (egg albumin, BSA) MEAA exhibited a significant percentage of inhibition (p < 0.001), which was parallel to diclofenac sodium (standard drug).…”

Ethnomedicinal Value of Antidiabetic Plants in Bangladesh: A Comprehensive Review

“…Examples of such studies are: Akter, Mahabub-Uz-Zaman, and Rahman, 2013; Al-Amin, Uddin, Rizwan, and Islam, 2013; Ali et al, 1993; Amran, Sultan, Rahman, and Rashid, 2013; Bhuyan, Rokeya, Masum, Hossain, and Mahmud, 2010; Borhanuddin, Shamsuzzoha, and Hussain, 1994b; A. Chowdhury and Biswas, 2012; A. R. Das, Mostofa, Hoque, Das, and Sarkar, 2010; Habib and Gafur, 2003; J. M. A. Hannan et al, 2003; E. Haque, Saha, Islam, and Islam, 2012; M. A. Hossain et al, 2012; Md Alamgir Hossain, Roy, Ahmed, Chowdhury, and Rashid, 2007b; M. Z. Hossain, Shibib, and Rahman, 1992; Islam et al, 2009; M. A. Islam et al, 2011; I. A. Jahan et al, 2009; Mostofa et al, 2007b; Mowl, Alauddin, Rahman, and Ahmed, 2009; Rafiq, Sherajee, Nishiyama, Sufiun, and Mostofa, 2009; Md Masudur Rahman, Hossain, Siddique, Biplab, and Uddin, 2012b; Md Mahfuzur Rahman, Sayeed, Haque, Hassan, and Islam, 2012; M. W. Rahman et al, 2005; Rokeya, Bhowmik, Khan, and Khter, 2009; M. G. Roy et al, 2010; Shahreen et al, 2012; Shibib, Khan, and Rahman, 1993; Sikder, Kaisar, Rahman, Hussain, and Rashid, 2011; Talukder, Khan, Uddin, Jahan, and Alam, 2012; Urmi et al, 2012; Zulfiker et al, 2011 [ 222 , 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 , 231 , 232 , 233 , 234 , 235 , 236 , 237 , 238 , 239 , 240 , 241 , 242 , 243 , 244 , 245 , 246 , 247 , 248 , 249 , 250 , 251 , 252 ]. These scientific studies emphasized the correlation among traditional use and the pharmacological properties of antidiabetic plants.…”