Unravelling the Phytochemistry of Ethnomedicinal Tree Pajanelia longifolia (Willd.) K. Schum.

Smitha, C. K.; Udayan, P. S.; Vaishnavi, M.

doi:10.1007/978-1-0716-2683-2_9

Cited by 2 publications

(2 citation statements)

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“…The red-to-green fluorescence ratio was highest for cells treated with camptothecin (mean value of 2.58), followed by GgLAE (mean value of 4.76), and untreated cells (mean value of 34.06). Overall, the results suggest that the GgLAE affects the red-to-green fluorescence ratio and mitochondrial membrane potential and induces mitochondrial dysfunction, which may have therapeutic applications in cancer treatment …”

Section: Resultsmentioning

confidence: 83%

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Multifaceted Anticancer Potential of Gnidia glauca (Fresen.) Gilg Leaf Alkaloids: Impact on Multiple Cellular Targets

Valleti,

Kumar,

Ramayanam

et al. 2024

ACS Omega

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Gnidia glauca (Fresen.) Gilg has demonstrated significant anticancer potential through multiple mechanisms, including apoptosis induction, as shown by the TUNEL assay against MCF-7 cells, modulation of tubulin polymerization, preservation of mitochondrial function indicated by the JC-1 assay, and inhibition of DNA polymerase α and β activities. Rationale for the present study is to investigate the potential anticancer properties of G. glauca leaf alkaloid extract. Fresh and healthy G. glauca leaves were cleaned, shade-dried, and the powder was defatted, extracted with 10% acetic acid in ethanol, and subjected for alkaloid extraction. The partially purified G. glauca leaf alkaloid extract was evaluated for its effects on tubulin polymerization, DNA polymerase activity, mitochondrial membrane potential, and apoptosis studies using human breast cancer (MCF-7) cells by flow cytometry. The extract was found to affect microtubule assembly in a concentration-dependent manner (15.125−250 μg/mL), indicating presence of alkaloids that function as spindle poison agents. Leaf alkaloid extract of G. glauca was also found to affect the mitochondrial membrane potential with IC 50 value 144.51 μg/mL, and inhibited DNA polymerase α and β activities dose dependently, thus potentially interfering with DNA replication and repair processes. Leaf alkaloid extract also showed the potential to induce DNA damage of 53.6%, albeit somewhat less than the standard drug camptothecin (64.94%) as confirmed by the TUNEL assay. Additionally, the GgLAE (IC 50 144.51 μg/mL) showed significant inhibition of MCF-7 cells proliferation after 24 h, revealing phase arrests in sub G0/G1, S, and G2/M. These findings suggest that G. glauca leaf alkaloid extract contains alkaloids that possess anticancer properties with multiple targets, making the plant a natural source for a promising phytochemical drug candidates for further evaluation in pre-clinical and clinical studies. Further investigations are warranted to determine the efficacy, safety, identification and characterization of the alkaloids, and evaluate and determine their potential applications in cancer therapy.

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Section: Resultsmentioning

confidence: 83%

“…Solasodine is a steroidal alkaloid with known anticancer and anti-inflammatory properties . Delphinine is a diterpene alkaloid that has been reported to possess antitumor activity. , …”

Section: Resultsmentioning

confidence: 99%