Unsaturated fatty acids promote proliferation via ERK1/2 and Akt pathway in bovine mammary epithelial cells

Yonezawa, Tomo; Haga, Satoshi; Kobayashi, Yasuhiko; Katoh, K.; Obara, Yoshiaki

doi:10.1016/j.bbrc.2007.12.190

Cited by 69 publications

(50 citation statements)

References 17 publications

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“…Additionally, concurring with another group we and another group demonstrated that GPR40 functions in cell proliferation and survival through ERK 1/2 and PI3K/Akt signaling pathway [1,2,9,10] and its coupling manner to G protein is mainly dependent on Gi/o, which is Pertussis Toxin (PTX)-sensitive, and to lesser extent Gq/11 [1,7] (Figure 1 right portion). It is striking that unsaturated LCFA, but not saturated, possesses strong proliferative and survival effects on mammary epithelial cells, including bovine mammary epithelial cells [1,7].…”

Section: Unsaturated Fatty Acid Enhances Proliferation and Survival Isupporting

confidence: 88%

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Supplementation of Long-Chain Fatty Acids with lactating Mammary Glands and Beef Cow has Potential Benefits

Yonezawa¹

2015

APAR

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Section: Unsaturated Fatty Acid Enhances Proliferation and Survival Isupporting

confidence: 88%

“…We've examined the effects of fatty acids on mammary epithelial cells and adipocytes including cow [1][2][3]. The ligands of two G Protein-Coupled Receptors (GPCRs), were recently identified as Long-Chain Fatty Acids (LCFAs) in the blood [4][5][6].…”

Section: Fatty Acid Receptorsmentioning

confidence: 99%

Supplementation of Long-Chain Fatty Acids with lactating Mammary Glands and Beef Cow has Potential Benefits

Yonezawa¹

2015

APAR

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“…The sequence of the primers used for GAPDH amplification was synthesized as described previously (Yonezawa et al 2008). Quantitative PCR was carried out using SYBR Premix Ex Taq (Takara Bio, Inc., Tokyo, Japan) on a DNA engine Opticon 2 Continuous Fluorescence Detector (MJ Research, Inc., Waltham, MA, USA).…”

Section: Rna Isolation and Quantitative Rt-pcrmentioning

confidence: 99%

“…cDNA was synthesized from 500 ng of total RNA as described previously (Yonezawa et al 2008). The primers used for PCR are as follows: GHRL: forward 5 0 -CAG-…”

Section: Rna Isolation and Quantitative Rt-pcrmentioning

confidence: 99%

Increased plasma ghrelin suppresses insulin release in wethers fed with a high-protein diet

Takahashi

Sato

Kato

et al. 2014

Journal of Endocrinology

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Ghrelin is a multifunctional peptide that promotes an increase of food intake and stimulates GH secretion. Ghrelin secretion is regulated by nutritional status and nutrients. Although a high-protein (HP) diet increases plasma ghrelin secretion in mammals, the mechanisms and the roles of the elevated ghrelin concentrations due to a HP diet have not been fully established. To clarify the roles of elevated acylated ghrelin upon intake of a HP diet, we investigated the regulation of ghrelin concentrations in plasma and tissues in wethers fed with either the HP diet or the control (CNT) diet for 14 days, and examined the action of the elevated plasma ghrelin by using a ghrelin-receptor antagonist. The HP diet gradually increased the plasma acylated-ghrelin concentrations, but the CNT diet did not. Although the GH concentrations did not vary significantly across the groups, an injection of ghrelinreceptor antagonist enhanced insulin levels in circulation in the HP diet group. In the fundus region of the stomach, the ghrelin levels did not differ between the HP and CNT diet groups, whereas ghrelin O-acyltransferase mRNA levels were higher in the group fed with HP diet than those of the CNT diet group were. These results indicate that the HP diet elevated the plasma ghrelin levels by increasing its synthesis; this elevation strongly suppresses the appearance of insulin in the circulation of wethers, but it is not involved in GH secretion. Overall, our findings indicate a role of endogenous ghrelin action in secretion of insulin, which acts as a regulator after the consumption of a HP diet.

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“…The composition of fatty acids that are stored in the fat depots reflects the previous action of SCD on substrates such as stearic acid and palmitic acid (Kim and Ntambi, 1999). MUFAs have been implicated as mediators in signal transduction and cellular differentiation, including neuronal differentiation (Bradley et al, 2008;Yonezawa et al, 2008). Recently, oleate has been shown to regulate food intake in the brain (Obici et al, 2002), and MUFAs may also influence apoptosis and mutagenesis in some tumors (Hardy et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells

Jiang

Liu

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 10 6 cells per dish). The test was divided into 6 groups with 6 replications per group. The 6 groups were treated with the following volumes of LPS (0.1 mg/mL): 0, 1, 1.5, 2, 4, and 8 µL. The cells were cultured for 24 h, and the total RNA was extracted from samples. Reverse transcription polymerase chain reaction was used to analyze SCD mRNA levels. With increasing LPS amounts, the SCD mRNA expression first decreased and then increased slightly; the expression was the lowest in the 2-µL LPS condition. The SCD mRNA levels from the 4-and 8-µL LPS conditions were slightly higher than that from the 2-µL LPS condition, but the difference was not significant (P > 0.05). The SCD mRNA level from the 2-µL LPS condition was obviously 2549 Stearoyl-coenzyme A desaturase mRNA ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 2548-2554 (2014) lower than that from the 0-, 1-, and 1.5-µL LPS condition, and the differences were significant (P < 0.05), and the SCD mRNA levels from the 0-, 1-, and 1.5-µL LPS conditions were not significantly different (P > 0.05). The SCD mRNA levels from the 4-and 8-µL LPS conditions were obviously lower than those from the 0-and 1-µL LPS conditions, and the differences were significant (P < 0.05).

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Unsaturated fatty acids promote proliferation via ERK1/2 and Akt pathway in bovine mammary epithelial cells

Cited by 69 publications

References 17 publications

Supplementation of Long-Chain Fatty Acids with lactating Mammary Glands and Beef Cow has Potential Benefits

Supplementation of Long-Chain Fatty Acids with lactating Mammary Glands and Beef Cow has Potential Benefits

Increased plasma ghrelin suppresses insulin release in wethers fed with a high-protein diet

Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells

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