Unsterile production of a polyhydroxyalkanoate copolymer by Halomonas cupida J9

Liu, Yujie; Zhao, Wanwan; Wang, Siqi; Huo, Kaiyue; Chen, Yaping; Guo, Hongfu; Wang, Shufang; Liu, Ruihua; Yang, Chao

doi:10.1016/j.ijbiomac.2022.10.275

Cited by 8 publications

(9 citation statements)

References 38 publications

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“…H. cupida J9 can produce the 3HB‐CoA and 3HDD‐CoA precursors via PHB synthesis pathway and de novo fatty acid synthesis pathway, respectively, and the two precursors are copolymerized to form SCL‐ co ‐MCL PHA by a low‐substrate‐specificity PhaC J9 (Figure 6). The pathway for SCL‐ co ‐MCL PHA biosynthesis in H. cupida J9 has been elucidated by Liu, Zhao et al, (2022).…”

Section: Resultsmentioning

confidence: 99%

“…The obtained hydrolysate was used as the carbon source for unsterile PHA production. The fermentation medium (pH 9.0) was prepared by adding all components of 80MM (Liu, Zhao, et al, 2022) to corn straw hydrolysate. Strain J9UΔ xylD ‐P8 xylA or J9U was inoculated into a flask containing 100 mL of fermentation medium and then fermented in a shaker at 37°C and 180 rpm for 72 h. Fermented cells were harvested for determining the PHA content and CDW (Liu Chen, et al, 2022; Liu, Zhao, et al, 2022; Liu et al, 2023; Zhang et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a halophile Halomonas cupida J9, which was isolated by our lab from high‐salt pharmaceutical manufacturing wastewater, was demonstrated to have an ability to synthesize SCL‐ co ‐MCL PHA from glucose or glycerol. Moreover, we proposed the potential pathways for PHA biosynthesis in H. cupida J9 and discovered a low‐specificity PHA synthase (PhaC) from H. cupida J9 (Liu, Zhao, et al, 2022). In this study, a scarless genome‐editing system with the upp gene as a counter‐selectable marker was established for H. cupida J9.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9

Wang,

Liu,

Guo

et al. 2024

Biotech & Bioengineering

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Microbial production of polyhydroxyalkanoate (PHA) is greatly restricted by high production cost arising from high‐temperature sterilization and expensive carbon sources. In this study, a low‐cost PHA production platform was established from Halomonas cupida J9. First, a marker‐less genome‐editing system was developed in H. cupida J9. Subsequently, H. cupida J9 was engineered to efficiently utilize xylose for PHA biosynthesis by introducing a new xylose metabolism module and blocking xylonate production. The engineered strain J9UΔxylD‐P8xylA has the highest PHA yield (2.81 g/L) obtained by Halomonas with xylose as the sole carbon source so far. This is the first report on the production of short‐ and medium‐chain‐length (SCL‐co‐MCL) PHA from xylose by Halomonas. Interestingly, J9UΔxylD‐P8xylA was capable of efficiently utilizing glucose and xylose as co‐carbon sources for PHA production. Furthermore, fed‐batch fermentation of J9UΔxylD‐P8xylA coupled to a glucose/xylose co‐feeding strategy reached up to 12.57 g/L PHA in a 5‐L bioreactor under open and unsterile condition. Utilization of corn straw hydrolysate as the carbon source by J9UΔxylD‐P8xylA reached 7.0 g/L cell dry weight (CDW) and 2.45 g/L PHA in an open fermentation. In summary, unsterile production in combination with inexpensive feedstock highlights the potential of the engineered strain for the low‐cost production of PHA from lignocellulose‐rich agriculture waste.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9

Wang,

Liu,

Guo

et al. 2024

Biotech & Bioengineering

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“…Moreover, our results showed that the highest PHA concentration (0.27 g/L) and PHA productivity (5.63 mg/(L·h)) was reached in the cultivation with 50 g/L of crude glycerol in 48 h. We also observed that the further increase of the waste substrate concentration (above 50 g/L) resulted in a decrease of PHAs productivity at all measured time‐points (Figure 2B ). However, Liu et al ( 2022 ) suggested that NaCl concentration can play a role in PHA production. The authors showed that pathways involved in salt tolerance can be blocked or weakened in H. cupida J9 cells when using glycerol as a feedstock.…”

Section: Resultsmentioning

confidence: 99%

Halomonas alkaliantarctica as a platform for poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) production from biodiesel‐derived glycerol

Możejko‐Ciesielska,

Moraczewski,

Czaplicki

2023

Environ Microbiol Rep

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Polyhydroxyalkanoates (PHAs) are biodegradable polyesters produced by a wide range of microorganisms, including extremophiles. These unique microorganisms have gained interest in PHA production due to their ability to utilise low‐cost carbon sources under extreme conditions. In this study, Halomonas alkaliantarctica was examined with regards to its potential to produce PHAs using crude glycerol from biodiesel industry as the only carbon source. We found that cell dry mass concentration was not dependent on the applying substrate concentration. Furthermore, our data confirmed that the analysed halophile was capable of metabolising crude glycerol into poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) copolymer within 24 h of the cultivation without addition of any precursors. Moreover, crude glycerol concentration affects the repeat units content in the purified PHAs copolymers and their thermal properties. Nevertheless, a differential scanning calorimetric and thermogravimetric analysis showed that the analysed biopolyesters have properties suitable for various applications. Overall, this study described a promising approach for the valorisation of crude glycerol as a future strategy of industrial waste management to produce high value microbial biopolymers.

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“…In Fig. 2, the authors described the metabolic pathways of PHA in Halomonas cupida J9 using various feedstocks such as glycerol, glucose, and fatty acids [163].…”

Section: Pha Production From Waste Oilsmentioning

confidence: 99%

Recent Development in Urban Polyhydroxyalkanoates Biorefineries

et al. 2023

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The widespread use of plastic items and their improper disposal harms not only physical environments but also human health globally. Therefore, a bioplastic or polymer that can completely vanish from the environment is the foremost priority. Bioplastic can be manufactured using raw materials from plants, animals, and microbes. Polyhydroxyalkaonates (PHAs) is a type of bioplastic which is naturally produced by microbes and importantly biodegradable as well as biocompostable. PHA production utilizing commercial sugars is an economically cost‐effective process. Due to urbanization and industrialization a huge amount of waste is generated around the world and its management is a major environmental issue. Urban wastes produced by wastewater treatment plant, agro/food‐processing industries, dairy industries, and even from household uses can be an economically sustainable feedstock for the synthesis of PHAs. The current status of bioplastic around the globe is summarized, and the urban waste potential as a feedstock for advances in PHA production technology is reviewed.

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Unsterile production of a polyhydroxyalkanoate copolymer by Halomonas cupida J9

Cited by 8 publications

References 38 publications

Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9

Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J9

Halomonas alkaliantarctica as a platform for poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) production from biodiesel‐derived glycerol

Recent Development in Urban Polyhydroxyalkanoates Biorefineries

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