Untargeted lipidomic analysis of plasma from obese women submitted to combined physical exercise

Abstract

This study aimed to determine the changes of lipidome in obese women undergoing combined physical exercise training. Fourteen adult women with obesity (mean BMI and age, 33 kg/m2 and 34 ± 5 years), were submitted to combined physical training (aerobic and strength exercises, alternately, 55 min at 75–90% of the maximum heart rate, 3 times a week) for 8 weeks. All participants were evaluated before and after the training intervention for lipidome, anthropometric measurements, muscle strength, and maximum oxygen consumption (VO2max). Untargeted liquid chromatography-mass spectrometry analyses allowed the identification of 1252 variables, of which 160 were significant (p < 0.05), and 61 were identified as molecular species of lipids. Volcano plot analysis revealed LPC(16:0p), LPC(18:0p), LPC(20:2), and arachidonic acid upregulated and PC(38:1p), PC(40:4), PC(40:4p) downregulated after combined physical exercise. From the results of the overall Principal component analysis (PCA), the major finding was SM(d18:1/20:0), arachidonic acid, and PC(40:6) species. Other changes included a reduction in waist circumference (Δ = − 2 cm) (p < 0.05), with no weight loss. In conclusion, 8-week of combined exercise training in obese women brought changes in different classes of lipids. This study provides further information to understand the effect of combined physical exercise on lipids related to obesity.

Metabolomics to Understand Alterations Induced by Physical Activity during Pregnancy

“…However, the levels of LPCs, like LPC(16:0) and LPC(18:2), exhibited a reduction after the training [56]. When exploring the plasma lipidome of women with obesity undergoing 8 weeks of supervised PA training, alternating between strength and aerobic exercises, a decline in the quantities of PC(40:6), PE(36:5), and SM(d18:1/20:0) was found [3]. It is well known that obesity increases the risk of insulin resistance; therefore, the increased concentration of UFAs within the phospholipid composition post-physical activity seen in this population may improve insulin resistance.…”

“…A sedentary lifestyle is a global health concern and a significant risk factor for mortality worldwide. In contrast, the recognition of the benefits of regular exercise dates back to Hippocrates and is recommended to reduce the risk of health conditions like diabetes, cancer, and obesity [1][2][3]. Metabolomics, a cutting-edge discipline in the field of systems biology, focuses on comprehensively analyzing the small-molecule metabolites present within biological systems and has gained popularity in characterizing physiological responses to exposures like physical activity (PA).…”

Mass Sportrometry: An annual look back at applications of mass spectrometry in sport and exercise science

“…In 2022, four studies were published using an exercise/sport‐based lipidomic approach, with two publications investigating obese subjects following aerobic and resistance exercise protocols, 103,104 one comparing trained individuals who were either overweight or normal weight, 87 and one involving human immunodeficiency virus‐positive patients 105 . San Martin et al 104 . prescribed combined physical training (aerobic and resistance) to obese females in a protocol including three sessions of alternating modalities each week for eight weeks.…”

“…In 2022, four studies were published using an exercise/sport‐based lipidomic approach, with two publications investigating obese subjects following aerobic and resistance exercise protocols, 103 , 104 one comparing trained individuals who were either overweight or normal weight, 87 and one involving human immunodeficiency virus‐positive patients. 105 San Martin et al.…”

“… 105 San Martin et al. 104 prescribed combined physical training (aerobic and resistance) to obese females in a protocol including three sessions of alternating modalities each week for eight weeks. Non‐targeted lipidomics analyses identified increases in three lysophosphatidylcholines and arachidonic acid, with decreases in three phosphatidylcholines.…”