Untargeted Metabolomics Reveals Alterations in the Primary Metabolites and Potential Pathways in the Vegetative Growth of Morchella sextelata

Deng, Kejun; Lan, Xiuhua; Fang, Qing; Li, Mengke; Xie, Guangzhong; Xie, Lu

doi:10.3389/fmolb.2021.632341

Cited by 15 publications

(10 citation statements)

References 19 publications

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“…A total of 394 metabolites (150 and 244 detected in the negative-ion and positive-ion mode, respectively) were detected by HPLC-MS/ MS. PCA analysis showed that the first (PC1) and second (PC2) principal components accounted for 35.60 and 18.37% of the total variance, respectively. The samples from the three developmental stages of M. sextelata were divided into three distinct regions based on the transcriptional characteristics (Figure 2A), consistent with findings from a previous metabolome-based research (Deng et al, 2021). This indicated that the changes of metabolites in the different developmental stages of M. sextelata were very notable, consistent with the results of the transcriptome analyses, suggesting that metabolome data can be used to study the developmental phenotypic changes of M. sextelata.…”

Section: Pca Analysis Of Metabolic Samples From M Sextelatasupporting

confidence: 86%

“…In the Up-Up model, the levels of L-citrulline and fumaric acid, which serve as auxiliary molecules associated with arginine metabolism, gradually increased with the growth of M. sextelata. This pattern has also been found in previous studies (Deng et al, 2021), and arginine is an essential metabolite in many cells and developmental processes (Winter et al, 2015). Moreover, fumaric acid is an essential component of the tricarboxylic acid cycle (TCA), implying the energy enhancement during the PMP.…”

Section: Identification Of Time-dependent Metabolite Modules Of M Sex...supporting

confidence: 82%

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Integration of Transcriptomics and Metabolomics for Understanding the Different Vegetative Growth in Morchella Sextelata

Deng¹,

Lan²,

Chen³

et al. 2022

Front. Genet.

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Morchella sextelata is an edible and medicinal fungus with high nutritional, medicinal, and economic value. Recently, M. sextelata has been produced through artificial cultivation in China, but its stable production remains problematic because the details of its growth and development process are limitedly understood. Herein, to investigate the dynamic process of M. sextelata development, we integrated the transcriptomics and metabolomics data of M. sextelata from three developmental stages: the young mushroom period (YMP), marketable mature period (MMP), and physiological maturity period (PMP). The results showed that the transcriptome changed dynamically at different stages and demonstrated the significant enrichment of pathways that regulate plant growth and development, such as N-glycan biosynthesis and carbon and purine metabolism. Similarly, small-molecule metabolites, such as D-fructose-1,6-biphosphate, which was upregulated during the YMP, dihydromyricetin, which was upregulated during the MMP, and L-citrulline, which was upregulated during the PMP, also showed phase-dependent characteristics. Then, combined analysis of the transcriptome data and metabolome traits revealed that the transcriptome may affect metabolic molecules during different growth stages of M. sextelata via specific enzymes, such as α-glucosidase and glucanase, which were included in two opposite transcriptome modules. In summary, this integration of transcriptomics and metabolomics data for understanding the vegetative growth of M. sextelata during different developmental stages implicated several key genes, metabolites, and pathways involved in the vegetative growth. We believe that these findings will provide comprehensive insights into the dynamic process of growth and development in M. sextelata and new clues for optimizing the methods for its cultivation application.

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Section: Pca Analysis Of Metabolic Samples From M Sextelatasupporting

confidence: 86%

Section: Identification Of Time-dependent Metabolite Modules Of M Sex...supporting

confidence: 82%

Integration of Transcriptomics and Metabolomics for Understanding the Different Vegetative Growth in Morchella Sextelata

Deng¹,

Lan²,

Chen³

et al. 2022

Front. Genet.

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“…The Morchella species currently cultivated in China include M. importuna , M. sextelata , and M. septimelata ( Liu et al, 2018 ). Among them, M. sextelata comprises >90% of the total area of Morchella cultivation ( Deng et al, 2021 ). However, the white mold disease (WMD) caused by Paecilomyces penicillatus (Ascomycetes) seriously harms Morchella ( He et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Dual RNA-Seq Analysis of the Interaction Between Edible Fungus Morchella sextelata and Its Pathogenic Fungus Paecilomyces penicillatus Uncovers the Candidate Defense and Pathogenic Factors

Tan

Liu

et al. 2021

Front. Microbiol.

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Morels (Morchella spp.) are economically important mushrooms cultivated in many countries. However, their production and quality are hindered by white mold disease because of Paecilomyces penicillatus infection. In this study, we aimed to understand the genetic mechanisms of interactions between P. penicillatus and Morchella. M. sextelata, the most prevalent species of Morchella in China, was inoculated with P. penicillatus; then, the expression profiles of both fungi were determined simultaneously at 3 and 6 days post-inoculation (dpi) using a dual RNA-Seq approach. A total of 460 and 313 differentially expressed genes (DEGs) were identified in P. penicillatus and M. sextelata, respectively. The CAZymes of β-glucanases and mannanases, as well as subtilase family, were upregulated in P. penicillatus, which might be involved in the degradation of M. sextelata cell walls. Chitin recognition protein, caffeine-induced death protein, and putative apoptosis-inducing protein were upregulated, while cyclin was downregulated in infected M. sextelata. This indicates that P. penicillatus could trigger programmed cell death in M. sextelata after infection. Laccase-2, tyrosinases, and cytochrome P450s were also upregulated in M. sextelata. The increased expression levels of these genes suggest that M. sextelata could detoxify the P. penicillatus toxins and also form a melanin barrier against P. penicillatus invasion. The potential pathogenic mechanisms of P. penicillatus on M. sextelata and the defense mechanisms of M. sextelata against P. penicillatus were well described.

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“…Accordingly, the mobilization of arginine from different cellular compartments allows its conversion into citrulline forming NO, which then regulates nitrogen metabolism, conidiation, and pathogenesis (Franco-Cano et al, 2021). This discovery is supported by the role of arginine as a stock of organic nitrogen (Deng et al, 2021). Therefore, arginine accumulation in A. niger might contribute to balancing nitrogen and carbon metabolism, or even by having a signaling function through NO.…”

Section: Lactosementioning

confidence: 99%

Assessing the intracellular primary metabolic profile of Trichoderma reesei and Aspergillus niger grown on different carbon sources

Borin¹,

Oliveira²

2022

Front. Fungal Biol.

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Trichoderma reesei and Aspergillus niger are efficient biological platforms for the production of various industrial products, including cellulases and organic acids. Nevertheless, despite the extensive research on these fungi, integrated analyses of omics-driven approaches are still missing. In this study, the intracellular metabolic profile of T. reesei RUT-C30 and A. niger N402 strains grown on glucose, lactose, carboxymethylcellulose (CMC), and steam-exploded sugarcane bagasse (SEB) as carbon sources for 48 h was analysed by proton nuclear magnetic resonance. The aim was to verify the changes in the primary metabolism triggered by these substrates and use transcriptomics data from the literature to better understand the dynamics of the observed alterations. Glucose and CMC induced higher fungal growth whereas fungi grown on lactose showed the lowest dry weight. Metabolic profile analysis revealed that mannitol, trehalose, glutamate, glutamine, and alanine were the most abundant metabolites in both fungi regardless of the carbon source. These metabolites are of particular interest for the mobilization of carbon and nitrogen, and stress tolerance inside the cell. Their concomitant presence indicates conserved mechanisms adopted by both fungi to assimilate carbon sources of different levels of recalcitrance. Moreover, the higher levels of galactose intermediates in T. reesei suggest its better adaptation in lactose, whereas glycolate and malate in CMC might indicate activation of the glyoxylate shunt. Glycerol and 4-aminobutyrate accumulated in A. niger grown on CMC and lactose, suggesting their relevant role in these carbon sources. In SEB, a lower quantity and diversity of metabolites were identified compared to the other carbon sources, and the metabolic changes and higher xylanase and pNPGase activities indicated a better utilization of bagasse by A. niger. Transcriptomic analysis supported the observed metabolic changes and pathways identified in this work. Taken together, we have advanced the knowledge about how fungal primary metabolism is affected by different carbon sources, and have drawn attention to metabolites still unexplored. These findings might ultimately be considered for developing more robust and efficient microbial factories.

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Untargeted Metabolomics Reveals Alterations in the Primary Metabolites and Potential Pathways in the Vegetative Growth of Morchella sextelata

Cited by 15 publications

References 19 publications

Integration of Transcriptomics and Metabolomics for Understanding the Different Vegetative Growth in Morchella Sextelata

Integration of Transcriptomics and Metabolomics for Understanding the Different Vegetative Growth in Morchella Sextelata

Dual RNA-Seq Analysis of the Interaction Between Edible Fungus Morchella sextelata and Its Pathogenic Fungus Paecilomyces penicillatus Uncovers the Candidate Defense and Pathogenic Factors

Assessing the intracellular primary metabolic profile of Trichoderma reesei and Aspergillus niger grown on different carbon sources

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