Untargeted Metabolomics to Explore the Bacteria Exo-Metabolome Related to Plant Biostimulants

Alacid, Verónica; Tomás-Barberán, Francisco A.; Garcı́a, Carlos; Palazón, P

doi:10.3390/agronomy12081926

Cited by 11 publications

(5 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A comparison of the functions of the identified metabolites revealed that endophytic PGPB was associated with diverse metabolic activities in sugarcane tissues, mainly carbohydrates and amino acids. The metabolic response of plants to inoculation with bacteria has been increasingly investigated in recent years, and few of those studies have analyzed the role of PGPB in shaping the tissue metabolome in carbon and N metabolism ( Garcia et al, 2022 ; Mhlongo et al, 2022 ). Amino acid metabolism is closely linked to carbohydrate and ammonium N (absorbed and synthesized from nitrate) metabolisms, which are the need for protein synthesis and secondary metabolism.…”

Section: Discussionmentioning

confidence: 99%

Endophytic nitrogen-fixing bacteria DX120E inoculation altered the carbon and nitrogen metabolism in sugarcane

Qin

Xie²,

Khan³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Endophytic nitrogen-fixing bacteria are versatile and widely distributed in plants. Numerous strains of endophytic nitrogen-fixing bacteria are used as biofertilizers to minimize the utilization of chemical fertilizers, improve nutrient use efficiency, increase crop productivity, and reduce environmental pollution. However, the mechanism underlying the interaction between nitrogen-fixing bacteria and plants is still unclear. So, the present study was planned to assess the effects of endophytic nitrogen-fixing bacteria on sugarcane by analyzing the changes in physiological and biochemical activities. In the current study, Klebsiella variicola DX120E, an endophytic nitrogen-fixing bacterium, was inoculated on sugarcane varieties B8 and ROC22 to evaluate the effects on nitrogen and carbon metabolism-related enzymatic activity and biomass. Results showed that DX120E inoculation improved the enzymatic activities related to gluconeogenesis and nitrogen metabolism increased the sugarcane plant’s height, cane juice Brix, biomass, chlorophyll, and soluble sugar content in sugarcane. Metabolomics analysis revealed that the metabolome modules were highly enriched in carbon and nitrogen metabolic pathways of strain-affected sugarcane than uninoculated control. The identified carbohydrates were associated with the glycolysis or gluconeogenesis and tricarboxylic acid (TCA) cycle in plants. Metabolomic profiling in the present investigation showed that carbohydrate metabolism is coordinated with nitrogen metabolism to provide carbon skeletons and energy to amino acid synthesis, and amino acid degradation results in several metabolites used by the citric acid cycle as an energy source. Moreover, differentially expressed metabolites of non-proteinogenic amino acids have a further complementary role to the action of endophytic nitrogen-fixing bacteria. Meanwhile, a significant difference in metabolites and metabolic pathways present in stems and leaves of B8 and ROC22 varieties was found. This study discovered the potential benefits of DX120E in sugarcane and suggested candidate regulatory elements to enhance interactions between nitrogen-fixing microbes and sugarcane.

show abstract

Section: Discussionmentioning

confidence: 99%

Endophytic nitrogen-fixing bacteria DX120E inoculation altered the carbon and nitrogen metabolism in sugarcane

Qin

Xie²,

Khan³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…A metatranscriptomic study was conducted to investigate the contributions of different nitrogen-fixing bacteria present in the maize inoculated liquid ( Gomez-Godinez et al, 2018 ). Metabolomics was applied to explore the exo-metabolome of three PGPB ( Pseudomonas putida IDE-01, Azospirillum brasilense IDE-06, and Bacilus megaterium IDE-14) in interaction with maize and rice ( Garcia et al, 2022 ). No twodimentional study was found on gene expression and metabolome of salinity stress-alleviating PGPB in interaction with plants.…”

Section: Omics Methods For Assaying Molecular Mechanisms Of Pgpbmentioning

confidence: 99%

“…In bacteria, enzymes related to SA biosynthesis are encoded by the NRPS gene cluster. In first step which is common in both bacteria and plants, chorismate is converted to isochorismate by isochorismate synthase, and then SA is produced from isochorismate using isochorismate pyruvate lyase ( Chen et al, 2019 ; Mishra and Baek, 2021 ).The metabolites related to the auxins and zeatins biosynthesis pathways were explored from three PGPB ( Pseudomonas putida IDE-01, Azospirillum brasilense IDE-06, and Bacilus megaterium IDE-14) in interaction with maize and rice ( Garcia et al, 2022 ).…”

Section: Biochemical and Genetic Mechanisms Of Salinity Stress-allevi...mentioning

confidence: 99%

Salinity stress endurance of the plants with the aid of bacterial genes

et al. 2023

View full text Add to dashboard Cite

The application of plant growth-promoting bacteria (PGPB) is vital for sustainable agriculture with continuous world population growth and an increase in soil salinity. Salinity is one of the severe abiotic stresses which lessens the productivity of agricultural lands. Plant growth-promoting bacteria are key players in solving this problem and can mitigate salinity stress. The highest of reported halotolerant Plant growth-promoting bacteria belonged to Firmicutes (approximately 50%), Proteobacteria (40%), and Actinobacteria (10%), respectively. The most dominant genera of halotolerant plant growth-promoting bacteria are Bacillus and Pseudomonas. Currently, the identification of new plant growth-promoting bacteria with special beneficial properties is increasingly needed. Moreover, for the effective use of plant growth-promoting bacteria in agriculture, the unknown molecular aspects of their function and interaction with plants must be defined. Omics and meta-omics studies can unreveal these unknown genes and pathways. However, more accurate omics studies need a detailed understanding of so far known molecular mechanisms of plant stress protection by plant growth-promoting bacteria. In this review, the molecular basis of salinity stress mitigation by plant growth-promoting bacteria is presented, the identified genes in the genomes of 20 halotolerant plant growth-promoting bacteria are assessed, and the prevalence of their involved genes is highlighted. The genes related to the synthesis of indole acetic acid (IAA) (70%), siderophores (60%), osmoprotectants (80%), chaperons (40%), 1-aminocyclopropane-1-carboxylate (ACC) deaminase (50%), and antioxidants (50%), phosphate solubilization (60%), and ion homeostasis (80%) were the most common detected genes in the genomes of evaluated halotolerant plant growth-promoting and salinity stress-alleviating bacteria. The most prevalent genes can be applied as candidates for designing molecular markers for screening of new halotolerant plant growth-promoting bacteria.

show abstract

“…In potato, the transcriptional analysis of Burkholderia phytofirmans PsJN examined stress-activated genes that had an impact on transcriptional regulation, reactive oxygen reduction, and homeostasis [ 35 ]. In corn and rice individuals, metabolomics was used to reveal the exo-metabolome of strains functioning as PGPB ( Pseudomonas putida IDE-01, Azospirillum brasilense IDE-06 and Bacillus megaterium IDE-14) [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of the Plant–Soil Relationship in Agricultural Production—With Particular Regard to PGPB Application and Phytoremediation

et al. 2023

View full text Add to dashboard Cite

Plant growth-promoting bacteria (PGPB) and other living organisms can help with the challenges of modern agriculture. PGPB offer ever-expanding possibilities for science and commerce, and the scientific results have been very advanced in recent years. In our current work, we collected the scientific results of recent years and the opinions of experts on the subject. Opinions and results on soil–plant relations, as well as the importance of PGPB and the latest related experiences, are important topics of our review work, which highlights the scientific results of the last 3–4 years. Overall, it can be concluded from all these observations that the bacteria that promote plant development are becoming more and more important in agriculture almost all over the world, thus, promoting more sustainable and environmentally conscious agricultural production and avoiding the use of artificial fertilizers and chemicals. Since many mechanisms of action, namely biochemical and operational processes, are still under investigation, a new emerging scientific direction is expected in the coming years with regard to PGPB, microbial, and other plant growth-stimulating substances, in which omics and microbial modulation also play a leading role.

show abstract

Untargeted Metabolomics to Explore the Bacteria Exo-Metabolome Related to Plant Biostimulants

Cited by 11 publications

References 52 publications

Endophytic nitrogen-fixing bacteria DX120E inoculation altered the carbon and nitrogen metabolism in sugarcane

Endophytic nitrogen-fixing bacteria DX120E inoculation altered the carbon and nitrogen metabolism in sugarcane

Salinity stress endurance of the plants with the aid of bacterial genes

The Role of the Plant–Soil Relationship in Agricultural Production—With Particular Regard to PGPB Application and Phytoremediation

Contact Info

Product

Resources

About