Untersuchung der molekularen Erkennung von Aminosäuren durch Cyclopeptide mit reflektometrischer Interferenzspektroskopie

Abstract

Bei der Suche nach selektiven Rezeptoren besteht ein vielversprechender Ansatz in der Immobilisierung von Cyclopeptiden auf Sensoroberflächen (siehe Bild) und anschließender Detektion niedermolekularer Analyte durch die reflektometrische Interferenzspektroskopie (RIfS). Diese innovative Methode bietet in Verbindung mit der kombinatorischen Chemie ein großes Potential für Anwendungen in der Sensorik.

Sensing A Paradigm Shift in the Field of Molecular Recognition: From Selective to Differential Receptors

“…Libraries of cyclic hexapeptides have been formed from which selective receptors for amino acids have been isolated 73. From this library of just under 6000 compounds, three cyclohexapeptides were identified (Scheme ) for their selectivity in binding L ‐arginine over L ‐lysine in neutral unbuffered aqueous solution.…”

Optically Active Cyclic Hexapeptides with Covalently Attached Pyrene Probes: Selective Alkaline Earth Metal Ion Recognition Using Excimer Emission

“…Cyclic peptides are important building blocks in life science and molecular material chemistry, operating in diverse areas such as physiological processes, supramolecular chemistry, or nanoscale materials. They are useful for the study of cell-adhesion processes, the construction of transmembrane nanotube channels in order to transport molecules and ions, and for molecular recognition of amino acids, anions, and cations . The labeling of biomolecules such as peptides with dye units enables the probing of intrinsic properties using emission and absorption spectroscopy.…”

Authors' reply: Randomized clinical trial of laparoscopic total (Nissen) versus posterior partial (Toupet) fundoplication for gastro-oesophageal reflux disease based on preoperative oesophageal manometry (Br J Surg 2008; 95: 57–63)

“…Epoxy‐terminated SAMs can react with molecules bearing amino groups. In this way it was possible to bind cyclopeptides to the glass surface for use as a molecular receptor190 and to determine binding constants between an immobilized tripeptide and vancomycin using confocal fluorescence detection 191. Hydrolysis of an epoxide‐functionalized SAM yields a diol‐functionalized SAM, which was used to develop a biosensor of immobilized nucleic acid aptamers to detect nonlabeled targets such as proteins 192…”