Untersuchungen mit J131-markiertem Albumin �ber Austauschvorg�nge zwischen Plasma und Liquor cerebrospinalis

Frick, E.; Scheid-Seydel, L.

doi:10.1007/bf01486246

Cited by 61 publications

(10 citation statements)

References 13 publications

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“…Dynamic studies using intravenously injected radio-labeled albumin have demonstrated that serum albumin is the precursor of CSF albumin and strongly support the use of CSF/serum albumin quotient (Q Alb ) to assess the blood-CSF barrier function (4). This protein is synthesized only by hepatocytes and is not catabolized within the central nervous system (CNS).…”

Section: Introductionmentioning

confidence: 93%

The Intrathecal Humoral Immune Response: Laboratory Analysis and Clinical Relevance

Sindic¹,

Antwerpen²,

Goffette³

2001

Clinical Chemistry and Laboratory Medicine

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In normal conditions, albumin and immunoglobulin (Ig)G in the cerebrospinal fluid (CSF) originate from the blood, and there is no antibody production within the central nervous system. Up to 20% of CSF proteins are intrathecally synthesized, but the major fraction is blood-derived. The CSF/serum albumin quotient (QAlb) is the best marker of the blood-CSF barrier function. The corresponding immunoglobulin quotients (QIGG, QIGA, QIGM) are not linearly related to QAlb and their correlations are defined by an hyperbolic equation. This equation is used to discriminate between a blood-derived and a locally produced fraction of immunoglobulins in case of an intrathecal humoral immune response. The detection of CSF-specific oligoclonal IgG is more sensitive than the quantitative comparison between QIGG and QAlb. A further step is the determination of antibody indices and the detection of specific oligoclonal antibodies by antigen-driven immunoblots. CSF analysis remains a cornerstone for the diagnosis of various neurological disorders, including multiple sclerosis and infectious diseases of the central nervous system.

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Section: Introductionmentioning

confidence: 93%

The Intrathecal Humoral Immune Response: Laboratory Analysis and Clinical Relevance

Sindic¹,

Antwerpen²,

Goffette³

2001

Clinical Chemistry and Laboratory Medicine

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“…has been shown to be present in serum when agar is employed as a supporting medium (Grabar & Williams 1953). Frick & Scheid-Seydel (1958) concluded from their studies with y-globulin tagged with 131I that y-globulin traverses the choroid plexus more slowly than the albumin, a dynamic equilibrium between serum and C.S.F. being achieved in about one hundred hours.…”

Section: Section Of Neurologymentioning

confidence: 99%

Immunity Mechanisms in Neurological Disease

Wh¹

1961

Proceedings of the Royal Society of Medicine

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“…The proteins present in the CSF are derived primarily from serum (5)(6)(7)(8). The passage of these proteins across the blood-CSF barrier is inversely related to size, resulting in relatively greater amounts of small proteins than large proteins in the CSF compared with amounts present in the serum (9).…”

Section: Introductionmentioning

confidence: 99%

Study of protein characteristics that influence entry into the cerebrospinal fluid of normal mice and mice with encephalitis.

Griffin

Giffels

1982

J. Clin. Invest.

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A B S T R A C T Entry of proteins into the cerebrospinal fluid (CSF) from the blood is partially determined by the size of the protein. To determine whether other characteristics of proteins influence CSF entry, proteins or protein fragments were iodinated, inoculated intravenously, and serum and CSF were sampled at later times. The Fc fragment of immunoglobulin G (IgG) did not enter the CSF significantly better than the Fab fragment suggesting that choroidal Fc receptors are not of importance for selective immunoglobulin entry. To determine the role of protein charge on entry, bovine serum albumin [isoelectric point (pI) = 3.9] was chemically altered to provide an albumin with an average pI of 6 (A-6) and another with a pl of 8.5 (A-8). All albumins were of the same size on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A-8 entered the CSF -10-fold better than the native albumin. A-6 was intermediate, entering approximately twofold better. At the time of increased CSF protein concentration during an acute viral encephalitis these differences were narrowed but not eliminated. It is concluded that charge is an important determinant of protein entry into the CSF.

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Untersuchungen mit J131-markiertem Albumin �ber Austauschvorg�nge zwischen Plasma und Liquor cerebrospinalis

Cited by 61 publications

References 13 publications

The Intrathecal Humoral Immune Response: Laboratory Analysis and Clinical Relevance

The Intrathecal Humoral Immune Response: Laboratory Analysis and Clinical Relevance

Immunity Mechanisms in Neurological Disease

Study of protein characteristics that influence entry into the cerebrospinal fluid of normal mice and mice with encephalitis.

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