Untersuchungen über einen obligat parasitischen Mikroorganismus mit lytischer Aktivität für Pseudomonas-Bakterien

Stolp, H.; Petzold, H

doi:10.1111/j.1439-0434.1962.tb02050.x

Cited by 119 publications

(61 citation statements)

References 8 publications

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“…Dominants are vibrio-formed but a few are ovoid or 5-formed. Such variations in form are in agreement with those of Bdellovibrio which have been isolated from soil and sewage [3,12,[14][15][16].…”

Section: Discussionsupporting

confidence: 74%

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Lethal Effect of Fresh Sea Water on Vibrio parahaemolyticus and Isolation of Bdellovibrio Parasitic against the Organism

Miyamoto¹,

Kuroda²

1975

Japanese Journal of Microbiology

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Halophilic Bdellovibrio, which is parasitic and lytic to Vibrio parahaemolyticus, was isolated from fresh sea water in the winter. It had a lethal effect on V. parahaemolyticus. The optimum temperature for multiplication ranged from 25 C to 30 C and growth was not observed at 35 C. Plaque numbers of the isolate reached a maximum in 17 hr under conditions of shaking at 25 C in autoclaved sea water supplemented with V. parahaemolyticus cells, and were as high as ten times the number of host cells.With respect to the host-suspended medium, the isolate multiplied in natural sea water ten times more than in Herbst's artificial sea water but did not grow in saline.V. parahaemolyticus, Vibrio alginolyticus and several species in the Vibrio genus were susceptible to the parasite on the basis of plaque formation but Escherichia coli and Staphylococcus aureus were not.In the summer Vibrio parahaemolyticus is widely detected in marine environments and food poisoning outbreaks due to the organisms are restricted to the summer months. In the winter, however, it has only rarely been isolated from sea water, sediments, plankton, fish or shellfish [1,7]. Many ecological data on V. parahaemolyticus have been accumulated but the question of their disappearance from sea environments in the winter does not seem to be answered in full.Microbial populations in natural environments can be affected not only by physical and chemical factors but also by biological ones. As one such biological factor, a unique parasitic bacterium, Bdellovibrio,which was found by Stolp and Petzold [15] and named by Stolp and Starr [16], is considered to play a significant role affecting the microbial flora of soil, water and sewage [11,13,16].In this pespect, Bdellovibrio may play a role in the ecology of V. parahaemolyticus in sea water in the winter. To elucidate this possibility, the authors have studied the distribution of Bdellovibrio, isolated two strains of "marine" Bdellovibrio from sea water in the winter and studied some of their characters. The present paper deals with the results of these studies. MATERIALS AND METHODSOrganisms. The host organisms used in this work were Vibrio parahaemolyticus A55 (Vp A55). The organisms used in studying the activity spectra of Bd. 5501 were V. parahaemolyticus, Vibrio alginolyticus 73-59-la, Vibrio costicola NCMB 701, Vibrio anguillarum NCMB 829, Vibrio ichthyodermis NCMB 1291, Escherichia coli UKT and Staphylococcus aureus

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Section: Discussionsupporting

confidence: 74%

“…As one such biological factor, a unique parasitic bacterium, Bdellovibrio,which was found by Stolp and Petzold [15] and named by Stolp and Starr [16], is considered to play a significant role affecting the microbial flora of soil, water and sewage [11,13,16].…”

mentioning

confidence: 99%

Lethal Effect of Fresh Sea Water on Vibrio parahaemolyticus and Isolation of Bdellovibrio Parasitic against the Organism

Miyamoto¹,

Kuroda²

1975

Japanese Journal of Microbiology

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“…The gram-negative bacterium Bdellovibrio bacteriovorus, an obligate endoparasite of gram-negative bacteria (Stolp and Petzold, 1962) is now extensively studied (Shilo, , 1973a(Shilo, , 1973b; Starr and Seidler, 1971;Starr, 1975;Starr and Stolp, 1976; Thomashow, 1979) as a possible simple model for both molecular and evolutionary aspects of parasitism.…”

Section: Introductionmentioning

confidence: 99%

Molecular parasitism in the Escherichia coli-Bdellovibrio bacteriovorus system: translocation of the matrix protein from the host to the parasite outer membrane.

Guerrini¹,

Romano²,

Valenzi³

et al. 1982

The EMBO Journal

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During the intracellular maturation in Escherichia coli of the parasite Bdellovibrio bacteriovorus the outer membrane, major protein I of E. coli (i.e., the matrix protein) becomes associated with the outer membrane of the emerging parasite cells. The binding properties of this protein with the outer membrane of the host and of the parasite are identical. An analogous phenomenon also occurs during Bdellovibrio parasitism on Klebsiella pneumoniae and on Salmonella typhimurium. Possible roles for this scavenging action of Bdellovibrio, and similar phenomena in other parasitic systems, are discussed.

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“…The description by Stolp & Petzold (1962) and by Stolp & Starr (1963) of an obligately ectoparasitic group of vibrios, Bdellovibrio bacteriovorus, highly specific in their ability to attach to and cause lysis of various Gram-negative bacteria and to form plaques on lawns of these host organisms, provided an intriguing new system for the study of mechanisms of bacterial lysis. Because of the ubiquitous occurrence of these bdellovibrios in soil and sewage, these organisms may play an important role in the ecological equilibrium of microbial populations in nature.…”

Section: Introductionmentioning

confidence: 99%

Lysis of Gram-Negative Bacteria by Host-Independent Ectoparasitic Bdellovibrio bacteriovorus Isolates

Shilo

Bruff

1965

Journal of General Microbiology

127

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SUMMARYMethods of isolation and nutritional requirements of Bdellovibrio bacteriovorusstrains capable of growth on host-free media are described. Such strains retained their parasitic capacities for various Gram-negative bacteria after many transfers in host-free media. In parasite +host suspensions the spectrum of host specificity of various bdellovibrio strains was considerably wider than that obtained by examination for plaques on host lawns. The growth conditions therefore affect the capacity of the bdellovibrios to attach to and lyse host organisms. A proteolytic exoenzyme formed by certain bdellovibrio strains digested heat-killed, acid-treated or EDTA-treated host organisms, but did not affect intact host organisms. High phosphate concentrations inhibited exoenzyme activity. The morphological sequence of lysis induced by exoenzyme-forming parasitic bdellovibrio strains suggested that this lysis was a two-stage process: the first stage was specific attachment of bdellovibrios with damage to the cell wall of the host organisms ; the second stage was non-specific digestion of cellular components by exoenzyme produced by the bdellovibrios.

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Untersuchungen über einen obligat parasitischen Mikroorganismus mit lytischer Aktivität für Pseudomonas-Bakterien

Cited by 119 publications

References 8 publications

Lethal Effect of Fresh Sea Water on Vibrio parahaemolyticus and Isolation of Bdellovibrio Parasitic against the Organism

Lethal Effect of Fresh Sea Water on Vibrio parahaemolyticus and Isolation of Bdellovibrio Parasitic against the Organism

Molecular parasitism in the Escherichia coli-Bdellovibrio bacteriovorus system: translocation of the matrix protein from the host to the parasite outer membrane.

Lysis of Gram-Negative Bacteria by Host-Independent Ectoparasitic Bdellovibrio bacteriovorus Isolates

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